Drug Toxicity Evaluation Based on Organ-on-a-chip Technology: A Review

Ye, Cong; Han, Xiahe; Wang, Youping; Chen, Zongzheng; Lu, Yao; Liu, Tingjiao; Wu, Zhengzhi; Jin, Yu; Luo, Yong; Zhang, Xiuli

doi:10.3390/mi11040381

Cited by 103 publications

(72 citation statements)

References 146 publications

(142 reference statements)

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“…Organ-on-a-chip platforms can recapitulate the complexity of tissues and organs to some extent by combining cellular and extracellular cues in the chip. In addition to promising features such as providing tissue barriers and hydrodynamic forces that organ-on-a-chip devices offer, the inner surface of organ-on-a-chip devices can be coated with extracellular matrix (ECM) components to resemble the native cellular microenvironment and improve cellular adhesion [ 3 , 5 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

et al. 2021

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Polydimethylsiloxane (PDMS) is a silicone-based synthetic material used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and assists the fabrication of complicated geometries at micro- and nano-scales, it does not optimally interact with cells for adherence and proliferation. Various strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate a living tissue microenvironment’s complexity. In organ-on-a-chip platforms, PDMS surfaces are usually coated by extracellular matrix (ECM) proteins, which occur as a result of a physical and weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to optimize coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (approximately three times higher) without showing any discernible difference in cell attachment between these two methods. These results suggested that such a surface modification can help coat extracellular matrix protein onto PDMS-based microfluidic devices.

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Section: Introductionmentioning

confidence: 99%

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

et al. 2021

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“…Moreover, rodents who got higher dosages of TZ (500 mg/kg) for 30 days exhibited rotations in learning and memory and declined cell reinforcement protections 10 . Besides, chronic administration of TZ (7.5 mg/kg) per diet for 90 days led to oxidative pressure and liver injury in grown-up rodents 3 .…”

mentioning

confidence: 98%

Ameliorating Effect of Vitamin E on Liver Damage Caused by Administering Tartrazine in Male Mice

Al-Shaikh

2021

Asian Journal of Pharmaceutical Research and Health Care

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Tartrazine is a common artificial food and pharmaceutical additive and has become a part of daily intake values. However, the actual daily intake is much higher than the recommended value. A higher intake is shown to exhibit carcinogenic, mutagenic, and allergic effects. The present work aimed to research whether antioxidant vitamin E (Vit E) therapy would impact liver damage induced by Tartar-Zine (TZ). Twenty-eight mice were used in this experimental study. Mice were categorized into four groups; group (A) in which mice served as control were orally administered distilled water for 28 days. Group (B) in which mice administered daily 100 mg/kg vitamin E orally for 28 days. Group (C) in which mice administered daily 300 mg/kg tartrazine dissolved in distilled water orally for 28 days. Group (D), mice received orally both 100mg/kg vitamin E and 300mg/kg tartrazine concomitantly for 28 days. Biochemical parameters in serum including liver function enzymes aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP), glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione S-transferase (GST) and catalase (CAT) had been inspected. Histological and immunological studies were done including Cyclooxygenase-2 (COX-2). Results showed that TZ treatment initiates hepatic disorders indicated by elevation of liver enzymes and oxidative stress markers in treated mice. Oral administration of Vit E diminished the activities of ALP, AST, and ALT compared to treated mice. Besides, Vit E effectively reduced oxidative stress as indicated by elevated GSH, SOD, GST, CAT, and decreased MDA. Histologically, TZ+Vit E delineated moderate potential improvement of hepatic tissue architecture. Immunologically revealed that TZ+Vit E treated mice showed reduction in COX-2 immuno-expression in hepatic cords sinusoids and hepatocytes versus TZ group. In conclusion, treatment with Vit E could improve all deviated biochemical, immunological, and histological changed induced by tartrazine consumption.

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“…In the review of Chong and co-workers, it was demonstrated that the microfluidic chip models could be used to detect drug toxicity. Several biomarkers of toxicity were determined by organ-on-a-chip technology [ 81 ].…”

Section: Utilization Of Skin-on-a-chip Systemsmentioning

confidence: 99%

Development of Skin-On-A-Chip Platforms for Different Utilizations: Factors to Be Considered

et al. 2021

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There is increasing interest in miniaturized technologies in diagnostics, therapeutic testing, and biomedicinal fundamental research. The same is true for the dermal studies in topical drug development, dermatological disease pathology testing, and cosmetic science. This review aims to collect the recent scientific literature and knowledge about the application of skin-on-a-chip technology in drug diffusion studies, in pharmacological and toxicological experiments, in wound healing, and in fields of cosmetic science (ageing or repair). The basic mathematical models are also presented in the article to predict physical phenomena, such as fluid movement, drug diffusion, and heat transfer taking place across the dermal layers in the chip using Computational Fluid Dynamics techniques. Soon, it can be envisioned that animal studies might be at least in part replaced with skin-on-a-chip technology leading to more reliable results close to study on humans. The new technology is a cost-effective alternative to traditional methods used in research institutes, university labs, and industry. With this article, the authors would like to call attention to a new investigational family of platforms to refresh the researchers’ theranostics and preclinical, experimental toolbox.

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Drug Toxicity Evaluation Based on Organ-on-a-chip Technology: A Review

Cited by 103 publications

References 146 publications

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

Ameliorating Effect of Vitamin E on Liver Damage Caused by Administering Tartrazine in Male Mice

Development of Skin-On-A-Chip Platforms for Different Utilizations: Factors to Be Considered

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