Dry film microchips for miniaturised separations

Abstract

In this work microfluidic devices were made from the dry film photoresist Ordyl SY330, characterised by optical and electron microscopy and used for electrophoretic separations. A simple and fast microfabrication process was developed for the fabrication of channels that are 50 microm wide and 30 microm in height, requiring only the use of an office laminator, a hot plate, an exposure source and mask and an electric drill to make four microdevices in less than 1 h. The optical properties of the photoresist were studied and the resist showed significant absorbance below 370 nm and 570-630 nm, and had an optical transmission of 80% between 400 and 550 nm. Fluorescence emission over the region of maximum transmission was low allowing these devices to be used for fluorescence detection at 488/512 nm. Electrophoretic separation of APTS and three derivatised sugars was performed in 20 mM phosphate buffer, pH 2.5 with efficiencies of the three sugars of 40,000 plates (2,100,000 plates/m) within 30 s at a field strength of 500 V/cm. The simple fabrication process also allowed microchannels to be easily filled with chromatography particles before sealing, avoiding the challenging task of slurry packing, and the potential of these devices for liquid chromatography was demonstrated by the extraction of fluorescein onto anion exchange particles.

Rapid and sensitive microbial analysis by capillary isotachophoresis with continuous electrokinetic injection under field amplified conditions

“…Microchip electrophoresis was carried out on a standard T cross‐shape as published by Guijt et al . The on‐chip visualization was performed on an inverted fluorescence microscope equipped with 4x objective (plan fluor, ∞‐W.0172, Nikon, Japan), Mercury light source was equipped with a filter cube (excitation/emission 488/525 nm).…”

Manufacturing and application of a fully polymeric electrophoresis chip with integrated polyaniline electrodes

“…The separations were conducted using a programmable 4-channel HV power supply constructed in-house in combination with an LED induced fluorescence detection system described previously. 19,20…”

Dry Film Resist Laminated Microfluidic System for Electrical Impedance Measurements

“…Vulto et al combined four-layer Ordyl DRF lamination for the fabrication of microfluidic channels with microelectrodes electroplated at the bottom substrate [5], whereas Mulloni et al fabricated a microfluidic device with three-level DFR and electrodes at the top and bottom plate [8]. Trantidou et al [9] as well as Guijt et al [10] developed a multilayer lamination process for simple microfluidic channels.…”