Dual colored mesoporous silica nanoparticles with pH activable rhodamine-lactam for ratiometric sensing of lysosomal acidity

Abstract

Alteration of lysosome acidity has been implicated in many biological events ranging from apoptosis to cancer metastasis, etc. Mesoporous silica nanoparticles doped with acid activable rhodamine-lactam and fluorescein isothiocyanate (FITC) were developed for ratiometric sensing of lysosomal pH changes in live cells with flow cytometry.

Localized Intracellular pH Measurement Using a Ratiometric Photoinduced Electron‐Transfer‐Based Nanosensor

“…[14] There have been some reports on the internalization of ratiometric fluorescence pH nanosensors by cells, [15] with localization of the nanoprobes in the cytoplasm [16] or within acidic organelles. [17] Other studies have reported measurements of a change in intracellular pH values using dual ratiometric fluorescence nanoprobes. [18] Recently, a ratiometric polymeric nanoprobe composed of three fluorophores has been reported for intracellular pH measurements.…”

Colloidal and spherical mesoporous silica particles: synthesis and new technologies for delivery applications

“…As expected, the reports are mostly from MCM-41 MSP and there are only few other materials such as HMM [85] and KIT-6 [111]. Almost all the reports are responsive to pH [111][112][113][114][115][116][117][118], light [85] or redox reactions [119,120], while cancer therapy continues to be the major concern. Different targeting molecules may be used such as folic acid (FA) [5,88,116], guanidine [111] or DNA [120].…”

Green synthesis of fluorescent copper nanoclusters for reversible pH-sensors

“…Recently, some groups [45,46] reported that some of the luminance of CuNCs came from aggregation induced emission (AIE). In order to deeply understand the mechanism of the luminescence of CEW@CuNCs, particle size distribution of the CuNCs in BR buffer with different pH (6,8,10,12) was investigated. As a result of this experiment, it almost had no significant change about the particle size of the CEW@CuNCs, which implied that the aggregation-induced emission was not the reason for the fluorescence increasing of the CuNCs at high pH values.…”

“…Up to now, fluorescent pH probes have been applied to numerous fields such as analytical application [6], biosensors [7,8] and bio-imaging [9]. Among these fluorescent pH sensors, they can be roughly divided into three categories: organic dye [10,11], semiconductor quantum dots (QDs) [12,13] and metal nano-materials [14,15]. However, most of the organic matter and QDs have toxicity, which limited its application range.…”