Dual effects of melatonin on barbiturate-induced narcosis in rats

Drago, Filippo; Frisina, Marialaura; Grech, M.; Nicolosi, Anna; Micale, Vincenzo; Nicosia, Angelo; Medico, M.; Foti, Francesco

doi:10.1016/s0304-3940(01)01578-6

Cited by 12 publications

(7 citation statements)

References 15 publications

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“…The melatonin was administered every morning at 08.00 AM. as proposed by Drago et al [14]. The melatonin was administered intraperitoneally in a single dose of 2 mg/kg -1 in order to avoid the neuromodulatory effect of melatonin.…”

Section: Experimental Designmentioning

confidence: 99%

The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation

Djordjević¹,

Kocić²,

Stoimenov³

et al. 2015

Adv Clin Exp Med

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Background. Microwaves from mobile phones are one of the environmental toxicants that are capable of compromising male fertility by inducing oxidative stress and apoptosis in the testes. Melatonin is a lipophilic tryptophan indole amine and a potent antioxidant. Objectives. The aim of the study was to evaluate the effect of melatonin treatment on oxidative stress parameters and DNA fragmentation in the testicular tissue of rats exposed to microwave radiation (4 h/day). Material and Methods. Adult Wistar rats were divided in 4 groups: I -treated with saline; II -treated with melatonin; III -exposed to microwaves; IV -exposed to microwaves and treated with melatonin. The melatonin (2 mg/kg ip) was administered daily. The animals were sacrificed after 20, 40 and 60 days. Results. Melatonin treatment prevented previously registered increases in malondialdehyde after only 20 days. Furthermore, it reversed the effects of microwave exposure on xanthine oxidase (after 40 days) and acid-DNase activity (after 20 days). However, neither protein carbonyl content nor catalase and alkaline Dnase activity were changed due to melatonin treatment. Conclusions. Melatonin exerts potent antioxidant effects in the testes of rats exposed to microwaves by decreasing the intensity of oxidative stress; it also reduces DNA fragmentation (Adv Clin Exp Med 2015, 24, 3, 429-436).

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Section: Experimental Designmentioning

confidence: 99%

The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation

Djordjević¹,

Kocić²,

Stoimenov³

et al. 2015

Adv Clin Exp Med

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“…Melatonin was administered intraperitoneally every morning at 08.00 AM, at a single dose of 2mg/kg body weightas proposed by Drago et al (11). In control rats, isotonic saline solution (equal to the volume of melatonin) was given intraperitoneallyeveryday during the follow up.…”

Section: Experimental Designmentioning

confidence: 99%

The effect of melatonin on the liver of rats exposed to microwave radiation

Djordjević¹,

Sokolović²,

Kocić³

et al. 2015

BLL

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Abstract:Objectives: We aimed to clarify if melatonin treatment (2 mg/kg i.p.) may favorably impact the liver tissue in rats exposed to microwave radiation. The experiment was performed on 84 six-weeks-old Wistar male rats exposed for 4h a day, for 20, 40 and 60 days, respectively, to microwaves (900 MHz, 100-300 microT, 54-160 V/m). Rats were divided in to four groups: I (control) -rats treated with saline, II (Mel) -rats treated with melatonin, III (MWs) -microwave exposed rats, IV (MWs + Mel) -MWs exposed rats treated with melatonin. We evaluated oxidative stress parameters (malondialdehyde and carbonyl group content), catalase, xanthine oxidase, deoxyribonuclease I and II activity. Background: Oxidative stress is the key mechanism of the microwave induced tissue injury. Melatonin, a lipophilic indoleamine primarily synthesized and released from the pineal gland is a powerful antioxidant. Results: Exposure to microwaves caused an increase in malondialdehyde after 40 (p < 0.01), protein carbonyl content after 20 (p < 0.05), catalase (p < 0.05) and xantine oxidase activity (p < 0.05) after 40 days. Increase in deoxyribonuclease I activity was observed after 60 days (p < 0.05), while deoxyribonuclease II activity was unaffected. Melatonin treatment led to malondialdehyde decrease after 40 days (p< 0.05), but surprisingly had no effect on other analyzed parameters. Conclusion: Melatonin exerts certain antioxidant effects in the liver of rats exposed to microwaves, by diminishing the intensity of lipid peroxidation (Fig. 6, Ref. 32).Text in PDF www.elis.sk.

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“…Melatonin was administered intraperitoneally (i.p.) every morning at 08.00 a.m., at a single dose of 2 mg/kg body weight as proposed by Drago et al (2001). In control rats, isotonic saline solution (equal to the volume of melatonin) was given intraperitoneally everyday during the follow up.…”

Section: Experimental Designmentioning

confidence: 99%

Melatonin protects rat thymus against oxidative stress caused by exposure to microwaves and modulates proliferation/apoptosis of thymocytes

Sokolović

Djordjević²,

Kocić³

et al. 2013

gpb

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The aim of the study was to evaluate the effect of melatonin on oxidative stress, DNA fragmentation, apoptsis and proliferation in thymus tissue of rats exposed to microwaves. Wistar rats were divided in four groups: I - treated with saline; II - treated with melatonin; III - microwaves exposed; IV - microwaves exposed and melatonin treated. Melatonin (2 mg/kg i.p.) was administered daily. Animals were sacrificed after 20, 40 and 60 days. A significant increase in malondialdehyde and carbonyl group content, as well as decrease in catalase and increase in xanthine oxidase activity were registered under microwave exposure. Melatonin prevented the increase in malondialdehyde and carbonyl group content, and reversed the effect on catalase and xanthine oxidase activity. Both, alkaline and acid DNase activity were increased due to microwave exposure. Furthermore, microwaves caused increase in apoptosis rate (detected using Annexin V-FITC/PI kit) and reduced proliferative capacity of thymocytes (induced by ConA). However, melatonin caused decrease in alkaline and acid DNase activity, decrease in apoptotic rate and increase in proliferation rate of thymocytes. Melatonin exerts protective effects on rat thymocytes by modulating processes of apoptosis and proliferation, and causes decrease in DNA fragmentation and oxidative stress intensity under exposure to microwaves.

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Dual effects of melatonin on barbiturate-induced narcosis in rats

Cited by 12 publications

References 15 publications

The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation

The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation

The effect of melatonin on the liver of rats exposed to microwave radiation

Melatonin protects rat thymus against oxidative stress caused by exposure to microwaves and modulates proliferation/apoptosis of thymocytes

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Dual effects of melatonin on barbiturate-induced narcosis in rats

Cited by 12 publications

References 15 publications

The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation

The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation

The effect of melatonin on the liver of rats exposed to microwave radiation

Melatonin protects rat thymus against oxidative stress caused by exposure to microwaves and modulates proliferation/apoptosis of thymocytes

Contact Info

Product

Resources

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Melatonin protects rat thymus against oxidative stress caused by exposure to microwaves and modulates proliferation/apoptosis of thymocytes