Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples

Zhang, Xian; He, Ke; Fang, Yun; Cao, Tong; Paudyal, Narayan; Zhang, Xiaofeng; Song, Houhui; Li, Xiaoliang; Fang, Weihuan

doi:10.1631/jzus.b1800085

Cited by 19 publications

(6 citation statements)

References 51 publications

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“…e immunoassay methods based on antigen-antibody reaction have the common characteristics of strong selectivity and high sensitivity and have become a hot topic in ZEN detection research, such as flexible pressure sensor (FPS) [16], self-powered temperature sensor (SPTS) [17], nano-hybrid-mediated photoelectrochemical immunoassay (NMPECIA) [18], and quantum dot-based photoelectrochemical immunoassay (QDPECIA) [19]. In addition, the established immunoassay methods, such as enzyme-linked immunosorbent assay (ELISA) [20], gold immunochromatographic assay (GICA) [21], and the time-resolved fluoroimmunoassay (TRFIA) [22], also have the advantages of fast, low cost, and on-site detection [15], fluorescence polarization immunoassay (FPIA) [23], immunosensor (IS) [24], and antibody microarray (AMA) [25] have the advantage of the highthroughput detection. ese immunoassay techniques play an important role in the rapid detection of ZEN.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Zearalenone Immunogen and Comparative Analysis of Antibody Characteristics

Wang

Zhang

et al. 2021

International Journal of Analytical Chemistry

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Background. This study aimed to explore the zearalenone (ZEN) immunogen synthesis method, immunogenicity, and antibody characteristics and to lay a foundation for the establishment of immunoassay methods for ZEN single residue and ZEN and its analogs total residue. Methods. Based on the molecular structure and active sites of ZEN, oxime active ester (OAE), condensation mixed anhydride (CMA), formaldehyde (FA), and 1,4-butanediol diglycidyl ether method (BDE) were designed and used for immunogen (ZEN-BSA) synthesis. The immunogens were identified by infrared (IR) and ultraviolet (UV) spectra and gel electrophoresis (SDS-PAGE) and were then used to immunize Balb/c mice to prepare ZEN polyclonal antibody (ZEN pAb). The titers and sensitivity of the ZEN pAb were determined by indirect noncompetitive ELISA (inELISA) and indirect competitive ELISA (icELISA), respectively, and its specificity was assessed by the cross-reaction test (CR). Results. ZEN-BSA was successfully synthesized, and the molecular binding ratios of ZEN to BSA were 17.2 : 1 (OAE), 14.6 : 1 (CMA), 9.7 : 1 (FA), and 8.3 : 1 (BDE), respectively. The highest inELISA titers of ZEN pAb of each group were 1 : (6.4 × 103) (OAE), 1 : (3.2 × 103) (CMA), 1 : (1.6 × 103) (FA), and 1 : (1.6 × 103) (BDE), respectively. The 50% inhibition concentrations (IC50) for ZEN by icELISA of each group were 11.67 μg/L (OAE), 16.29 μg/L (CMA), 20.92 μg/L (FA) and 24.36 μg/L (BDE), respectively. ZEN pAb from the mice immunized with ZEN-BSA (OAE) and ZEN-BSA (CMA) had class broad specificity to ZEN and its analogs. The CRs of ZEN pAb with α-ZAL, β-ZAL, α-ZOL, β-ZOL, and ZON were 36.53%, 16.98%, 64.33%, 20.16%, and 10.66%, respectively. ZEN pAb from the mice immunized with ZEN-BSA (FA) and ZEN-BSA (BDE) had high specificity for ZEN. The CRs of ZEN pAb with its analogs were all less than 1.0%. Conclusion. This study demonstrated that the preparation of the class broad-specificity antibodies of ZEN and its analogs can be achieved by immunizing animals with the immunogen ZEN-BSA prepared by the OAE method, while the preparation of highly specific antibodies can be achieved by immunizing animals with the immunogen ZEN-BSA prepared by the FA method. These findings lay the material and technical foundation for immunoassay of ZEN single residue and ZEN and its analogs total residue.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Zearalenone Immunogen and Comparative Analysis of Antibody Characteristics

Wang

Zhang

et al. 2021

International Journal of Analytical Chemistry

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“…Corn, rice, peanuts (Chen et al, 2016) Ochratoxin A, zearalenone 0.32, 0.58 ng/ml Corn, wheat, feedstuff (Zhang et al, 2018) Aflatoxin B1, zearalenone, trichothecene 0.5, 2, 30 ng/ml Maize, cereal (Xu et al, 2018) gentamicin sulfate, neomycin sulfate, kanamycin sulfate 0.737, 8.971, 11.110 ng/ml Milk (Sun et al, 2018) 3-amino-2-oxazolidinone, semicarbazide, 3-amino-5-methylmorpholino-2oxazolidinone, 1-aminohydantoin 0.5, 0.75 ng/ml Fish (Wang, Liu et al, 2018) Parathion, triazophos (Taheri et al, 2016) Fumonisin B1, fumonisin B2, fumonisin B3 11.24 ng/ml Maize (Yao et al, 2017) Aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2 0.21 ng/g Maize (Zhang, Song et al, 2017) Ochratoxin A, ochratoxin B, ochratoxin C 0.005, 0.001, 0.001 ng/ml Millet, maize Ochratoxin A, ochratoxin B, ochratoxin C 0.02, 0.04, 0.03 pg/ml Water (Qileng et al, 2018) Ochratoxin A, ochratoxin B, ochratoxin C 0.1, 0.5, 0.5 ng/ml Water (Qileng et al, 2020) (Continues) , 2019). This test demonstrated higher sensitivity than other reported mycotoxin ICA methods (reached up to 5, 20, and 10 ng/ml for OTA, FB 1 , and ZEN within 10 min).…”

Section: µG/kgmentioning

confidence: 99%

“…The biggest advantage of colored nanoparticles is that they can be simply visualized by naked eyes, offering a convenient qualitative screening tool. Thus, colored nanoparticles like gold nanoparticles (AuNPs) are extensively used in multi‐T lines ICA for synchronous detection of food contaminants, such as mycotoxins (Chen et al., 2016; Hou et al., 2020; Kim et al., 2014; Li et al., 2013; Zhang et al., 2018), antibiotics (Sun et al., 2018; Taranova et al., 2015; Wang, Liu et al., 2018; Zhang et al., 2009), and pesticides (Liu et al., 2018; Xing et al., 2015). However, with the number of test lines increasing, the distance between two adjacent test lines get closer.…”

Section: Strategies and Applications Of Mobas In Food Safety Analysismentioning

confidence: 99%

Multiplex optical bioassays for food safety analysis: Toward on‐site detection

Guan

Jin

et al. 2022

Comp Rev Food Sci Food Safe

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Food safety analysis plays a significant role in controlling food contamination and supervision. In recent years, multiplex optical bioassays (MOBAs) have been widely applied to analyze multiple hazards due to their efficiency and low cost. However, due to the challenges such as multiplexing capacity, poor sensitivity, and bulky instrumentation, the further application of traditional MOBAs in food screening has been limited. In this review, effective strategies regarding food safety MOBAs are summarized, such as spatial‐resolution modes performed in multi‐T lines/dots strips or arrays of strip/microplate/microfluidic chip/SPR chip and signal‐resolution modes employing distinguishable colorimetric/luminescence/fluorescence/surface plasma resonance/surface‐enhanced Raman spectrum as signal tags. Following this, new trends on how to design engineered sensor architecture and exploit distinguishable signal reporters, how to improve both multiplexing capacity and sensitivity, and how to integrate these formats into smartphones so as to be mobile are summarized systematically. Typically, in the case of enhancing multiplexing capacity and detection throughput, microfluidic array chips with multichannel architecture would be a favorable approach to overcome the spatial and physical limitations of immunochromatographic assay (ICA) test strips. Moreover, noble metal nanoparticles and single‐excitation, multiple‐emission luminescence nanomaterials hold great potential in developing ultrasensitive MOBAs. Finally, the exploitation of innovative multiplexing strategy hybridized with powerful and widely available smartphones opens new perspectives to MOBAs. In future, the MOBAs should be more sensitive, have higher multiplexing capacity, and easier instrumentation.

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“…[30] Therefore, the feed samples were pretreated and the pretreatment methods were adopted according to previous studies. [31,32] The feed samples were bought from a local market and confirmed as negative with ELISA. The BACfree feed samples were ground into a powder and screened.…”

Section: Sample Preparation and Analysis Of Feed Samplesmentioning

confidence: 99%

Quantum dot‐based fluorescence immunosorbent assay for the rapid detection of bacitracin zinc in feed samples

et al. 2022

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Bacitracin zinc (BAC), a polypeptide antibiotic, is utilized as a feed additive due to its ability to promote growth in animals. However, the abuse of BAC can lead to a great threat to food safety. Therefore, there is an urgent need to develop a rapid and sensitive detection method. In this study, a monoclonal antibody (mAb) against BAC with excellent sensitivity and specificity was obtained. For the first time, quantum dots (QDs) were conjugated with the prepared mAb against BAC and rabbit anti‐mouse antibody to fabricate a direct and an indirect competitive fluorescence‐linked immunosorbent assay (dc‐FLISA and ic‐FLISA) to detect BAC. The IC50 of dc‐FLISA and ic‐FLISA were 0.28 ng/ml and 0.17 ng/ml, respectively. The limits of detection were 0.0016 ng/ml and 0.001 ng/ml, respectively, and the detection ranges were 0.0016–46.50 ng/ml and 0.001–35.65 ng/ml, respectively. In addition, the recovery rate of the two methods ranged from 93.5% to 112.0%, and the coefficient of variation (CV) was less than 10%. Therefore, the methods developed in this work have the merits of low cost, simple operation, and high sensitivity, which provide an effective analytical tool for BAC residue detection in feed samples.

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Dual flow immunochromatographic assay for rapid and simultaneous quantitative detection of ochratoxin A and zearalenone in corn, wheat, and feed samples

Cited by 19 publications

References 51 publications

Synthesis of Zearalenone Immunogen and Comparative Analysis of Antibody Characteristics

Synthesis of Zearalenone Immunogen and Comparative Analysis of Antibody Characteristics

Multiplex optical bioassays for food safety analysis: Toward on‐site detection

Quantum dot‐based fluorescence immunosorbent assay for the rapid detection of bacitracin zinc in feed samples

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