Dual Function of PI(4,5)P2 in Insulin-Regulated Exocytic Trafficking of GLUT4 in Adipocytes

Structural Determinants for Light-Dependent Membrane Binding of a Photoswitchable Polybasic Domain

“…Translocation of peripheral membrane proteins to the plasma membrane (PM) is a vital process for many cellular events, including G protein-coupled receptor signaling, vesicular trafficking, , store-operated Ca 2+ entry (SOCE), − and GTPase-related signaling . To spatiotemporally control the membrane binding events of target proteins in cells, we previously designed an optogenetic tool called OptoPB, which consists of a blue light reactive photoswitch LOV2, and Rit-PB, a PM-binding polybasic (PB) peptide derived from Ras-like without CAAX1 (Rit1), a small GTPase (Figure A). , LOV2, the second LOV domain of Avena sativa phototropin1, contains a flavin mononucleotide (FMN)-binding Per-ARNT-Sim (PAS) core and a carboxy-terminal helix (Jα).…”