Dual-function oleaginous biocatalysts for non-sterile cultivation and solvent-free biolipid bioextraction to reduce biolipid-based biofuel production costs

Hwangbo, Myung; Gill, Jason J.; Young, Ralph H.; Chu, Kung-Hui

doi:10.1016/j.scitotenv.2020.143969

Cited by 2 publications

(2 citation statements)

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“…21 This strategy is suitable for fermentation using extremophilic microorganisms or challenging substrates. 14,21,22 Due to the complex molecular structure of lignin, most natural microbial strains cannot utilize lignin as the sole carbon source. 23 Li et al 24 screened several commonly used microbial strains (e.g., Rhodococcus opacus PD630, Rhodococcus jostii RHA1, and R. jostii RHA1 VanA − ) for their ability to degrade lignin and found that R. jostii RHA1 showed the best performance in terms of lignin bioconversion, by which lignin degradation reached to ∼23%.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In order to save energy and operation time, non-sterilization fermentation represents an emerging energy-efficient strategy, which has been applied in various studies to produce value-added compounds, including polyhydroxyalkanoates (PHA), microbial oil, and 2,3-butanediol . This strategy is suitable for fermentation using extremophilic microorganisms or challenging substrates. ,, Due to the complex molecular structure of lignin, most natural microbial strains cannot utilize lignin as the sole carbon source . Li et al screened several commonly used microbial strains (e.g., Rhodococcus opacus PD630, Rhodococcus jostii RHA1, and R.…”

Section: Introductionmentioning

confidence: 99%

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Enhancing Lignin Dispersion and Bioconversion by Eliminating Thermal Sterilization

Zhao

Meng

Senanayake

et al. 2022

ACS Sustainable Chem. Eng.

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Thermal sterilization is widely applied in fermentation to ensure a pure culture. In this study, a facile and energyefficient strategy by eliminating thermal sterilization (ETS) was unveiled for upgrading lignin bioconversion. Through alkaline solubilization and neutralization, lignin dispersion in aqueous fermentation media was significantly enhanced by ETS. Small-angle X-ray scattering and dynamic light scattering analyses indicated that the lignin colloid size was dramatically reduced. Compared to 20.5 wt % lignin precipitation during the conventional thermal sterilization, precipitated lignin was not observed within the ETS medium. 31 P NMR characterization demonstrated an 11.7% increase of phenolic OH in ETS lignin. Ionization of phenolic OH presented more negatively charged groups, strengthening electrostatic repulsion, resulting in smaller colloidal particles. Interestingly, the pure culture of Rhodococcus opacus PD630 was achieved within the ETS medium due to the lack of lignin degradation ability with most natural microbes. R. opacus PD630 cell amount, lignin degradation, and lipid production by ETS increased by 330, 16.6, and 20.7%, respectively. Overall, an energy-efficient ETS strategy that promoted lignin dispersion and bioconversion significantly is reported in this study.

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