Dual-functional Nanomaterials Polyo-phenylenediamine and Ru–Au Complement Each Other to Construct an Electrochemical and Electrochemiluminescent Dual-Mode Aptamer Sensor for Sensitive Detection of Alternariol

Abstract

To sensitively monitor trace amounts of alternariol (AOH) in fruits, a dual-mode aptamer sensor utilizing the dual-function nanomaterial PoPD/Ru–Au was developed. This sensor provides both electrochemical (EC) and electrochemiluminescence (ECL) signals, which can greatly avoid the potential false positive of the traditional single signal, thus enhancing the accuracy and reliability of detection results. Polyo-phenylenediamine (PoPD), known for its favorable EC response, can also assist in enhancing the ECL behavior of Ru–Au. Furthermore, Ru–Au demonstrates excellent ECL performance and effectively activates K2S2O8 to amplify the EC response of PoPD. The complementary effect of the two can effectively amplify the final detection signal. Additionally, the PoPD/Ru–Au nanomaterial exhibits excellent electrical conductivity, further enhancing the EC and ECL response signals. The experimental results demonstrate that the EC detection range of AOH was 0.01–100 ng/mL, while the ECL detection range was 0.001–100 ng/mL, both exhibiting a satisfactory linear relationship. Therefore, the mutual verification of the detection results can be highly realized, and the purpose of avoiding wrong detection can be achieved.

Enzyme-free electrochemical sensor platforms based on transition metal nanostructures for clinical diagnostics

“…Numerous enzymes or mediator-free amperometric sensor platforms for sensing lactic acid were intensively developed to overcome the challenges with enzyme-based sensors. 60–62…”

“…Numerous enzymes or mediator-free amperometric sensor platforms for sensing lactic acid were intensively developed to overcome the challenges with enzyme-based sensors. [60][61][62] The design of transition metal nanostructures with high surface energy has a strong potential in the eld of biosensing research. [63][64][65] In recent years, metal chalcogenides, metal phosphides, and nanocomposites with noble metal nanoclusters and carbon nanomaterials, nanocomposites were developed for sensing lactic acid under biological uids.…”

Quartz crystal microbalance–based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples

“…These advantages ensure the aptamer ligand is a well-targeted and target-specific interaction molecule in the designed sensor system. Various aptamer ligands have been effectively immobilized on the differently prepared composite magnetic materials and used for the isolation and detection of target bacteria from food samples [ 13 , 21 , 42 – 44 ].…”

Solvent Regulation Induced Cathode Aggregation-Induced Electrochemiluminescence of Tetraphenylethylene Nanoaggregates for Ultrasensitive Zearalenone Analysis

“…Consequently, it is urgently necessary to develop a simple operation, low-cost, and easy-to-control approach for ZEN detection. Prior studies suggest that electrochemiluminescence (ECL) aptasensor has the advantages of a low detection limit, low cost, and high sensitivity in food safety, biological analysis, and other fields. − Moreover, the ECL aptasensor has rarely been reported in the detection of ZEN. Based on this, we hope to build an ECL aptasensor to achieve the ultrasensitive detection of ZEN.…”