Dual Inhibition of Salmonella enterica and Clostridium perfringens by New Probiotic Candidates Isolated from Chicken Intestinal Mucosa

Abstract

The poultry industry is the fastest-growing agricultural sector globally. With poultry meat being economical and in high demand, the end product’s safety is of importance. Globally, governments are coming together to ban the use of antibiotics as prophylaxis and for growth promotion in poultry. Salmonella and Clostridium perfringens are two leading pathogens that cause foodborne illnesses and are linked explicitly to poultry products. Furthermore, numerous outbreaks occur every year. A substitute for antibiotics is required by the industry to maintain the same productivity level and, hence, profits. We aimed to isolate and identify potential probiotic strains from the ceca mucosa of the chicken intestinal tract with bacteriocinogenic properties. We were able to isolate multiple and diverse strains, including a new uncultured bacterium, with inhibitory activity against Salmonella Typhimurium ATCC 14028, Salmonella Abony NCTC 6017, Salmonella Choleraesuis ATCC 10708, Clostridium perfringens ATCC 13124, and Escherichia coli ATCC 25922. The five most potent strains were further characterized for their probiotic potential (i.e., sensitivity to antibiotics and tolerance to gastrointestinal physicochemical conditions). Our analyzed lactobacilli strains exhibited some interesting probiotic features while being inhibitory against targeted pathogens.

Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges

“…Several probiotics have been found effective in improving animal performance and preventing diseases (Bajagai et al 2016). Certain L. salivarius strains have also antimicrobial activity in animals, preventing Salmonella enteritidis colonization in chicken (Pascual et al 1999;Lone et al 2021), and reducing and preventing enteropathogen infections in piglets (Kumar et al 2010;Zhou et al 2020;Moturi et al 2021) and calves (Maldonado et al 2018).…”

Characterization and in vitro assessment of probiotic potential of Lactiplantibacillus plantarum BXM2 from fermented honey passion fruit beverage

“…Strain BXM2 showed resistance against vancomycin, gentamicin, kanamycin and ciprofloxacin, while was intermediary sensitive to oxacillin and ofloxacin, and sensitive to the rest 11 kinds of antibiotics. It has been reported that many Lactobacillus species exhibit intrinsically resistance to several antibiotics, including vancomycin, gentamicin, kanamycin, and ciprofloxacin (Gueimonde et al., 2013; Lone et al., 2021). Therefore, resistance against these antibiotics can be considered as Lactobacillus intrinsic and nontransmissible antibiotic resistance and do not affect the safety of L. plantarum BXM2.…”

Long-term administration of royal jelly regulates age-related disorders and improves gut function in naturally aging mice

“…Ligilactobacillus exhibits promising potential as a probiotic due to its antimicrobial activity, anti-inflammatory properties, and ability to maintain a healthy balance of gut bacteria. 26,27 Ileibacterium, Turicibacter, and Romboutsia were negatively correlated with SOD, GSH-pX, Klotho, and Sirt7. Conversely, these bacteria showed a positive correlation with pro-inflammatory factors TNF-α, IL-6, IL-1β, TG, and GSP.…”