Dual Ratiometric SERS and Photoacoustic Core–Satellite Nanoprobe for Quantitatively Visualizing Hydrogen Peroxide in Inflammation and Cancer

Abstract

Excessive production of oxidative species alters the normal redox balance and leads to diseases,s uch as chronic inflammation and cancer.O xidative species are short-lived species,w hich makes direct, precise,a nd real-time measurements difficult. Herein, we report an ovel core-satellite gold nanostructure for dual, ratiometric surface-enhanced Raman scattering (SERS) and photoacoustic (PA) imaging to enable the precise detection of inflammation/cancer-related H 2 O 2 .The combination of H 2 O 2-activated second near-infrared (NIR-II) PA imaging and SERS imaging enables the differentiation between the inflamed region and normal tissue with high accuracy.The mesoporous silica shell of the nanoprobe could be used to deliver drugs to the target area to precisely treat disease.T herefore,t his core-satellite nanostructure can not only quantitatively and precisely monitor H 2 O 2 produced in inflammation, tumor,and osteoarthritis in rabbits in real-time, but can also be used to track the progress of the antiinflammatory treatment in real-time.

In Situ Monitoring of Hydrogen Peroxide Released from Living Cells Using a ZIF-8-Based Surface-Enhanced Raman Scattering Sensor

“…H 2 O 2 is one of the most stable and abundant reactive oxygen species (ROS) molecules in living organisms, playing a crucial role in redox signaling processes. , While the normal level of H 2 O 2 is essential for cell survival, excessive production leads to oxidative damage to cells and causes various physical diseases, such as Alzheimer’s disease, cardiovascular dysfunctions, inflammation, and even cancer. − H 2 O 2 can also easily transfer across plasma membranes from living cells, involving in transmembrane redox signaling processes. , Consequently, establishing an approach for sensitive and real-time monitoring of H 2 O 2 secreted from living cells is not only of great importance for early diagnosis but also for understanding the mechanism of membrane diffusion of H 2 O 2 and the behavior among cells. , To date, various analytical techniques were applied, such as fluorescence, electrochemistry, chemiluminescence, and so on, for the detection of H 2 O 2 . − Despite important progress, several factors hamper their widespread use, such as relatively long reaction time, low sensitivity, and self-interference. ,− Therefore, there is still plenty of room to explore efficient and interference-free approaches with high sensitivity for the determination of H 2 O 2 levels to aid in early disease diagnosis.…”

“…4,7 Consequently, establishing an approach for sensitive and real-time monitoring of H 2 O 2 secreted from living cells is not only of great importance for early diagnosis but also for understanding the mechanism of membrane diffusion of H 2 O 2 and the behavior among cells. 7,8 To date, various analytical techniques were applied, such as fluorescence, electrochemistry, chemiluminescence, and so on, for the detection of H 2 O 2 . 9−13 Despite important progress, several factors hamper their widespread use, such as relatively long reaction time, low sensitivity, and self-interference.…”

Enhanced porphyrin-based fluorescence imaging-guided photodynamic/photothermal synergistic cancer therapy by mitochondrial targeting

“…It proved that PTPF-MitP retained highly sensitive recognition and catalysis toward H 2 O 2 , accompanying the burst of O 2 . To simulate tumor conditions where H 2 O 2 was continuously produced within tumor tissues [40,41], the catalytic activity of PTPF-MitP was further investigated by the repetitive addition of H 2 O 2 three times, demonstrating that PTPF-MitP retained its persistent catalytic activity (Fig. 3c).…”

A H2O2-responsive theranostic platform for chemiluminescence detection and synergistic therapy of tumors

“…This feature could be used to track tumors and design tumor-targeted drug delivery. [11][12][13][14] However, how to detect and treat tumors more accurately is still a big challenge.…”