Dual Requirement of Walker Carcinosarcoma 256
            <i>in vitro</i>
            for Asparagine and Glutamine

Neuman, Robert E.; McCoy, Thomas A.

doi:10.1126/science.124.3212.124

Cited by 95 publications

(20 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Yet, by contrast, ceils of lymphoma 6C3HED newly placed in culture and cells of certain other tumors too, fail to proliferate in tissue culture unless an external source of L-asparagine is provided. Neuman and McCoy (11) found that in addition to other amino acids, L-asparagine was necessary in vitro for the growth of the Walker carcinosarcoma 256, significantly, in view of the present findings, a guinea pig serum sensitive ceil line (12). McCoy and coworkers later showed that 2 other tumors, the Jensen sarcoma and a rat hepatoma induced by methyldimethylaminoazo benzene, included L-asparagine amongst their growth requirements in culture (13,14).…”

Section: Discussionmentioning

confidence: 99%

EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS

Broome

1963

The Journal of Experimental Medicine

155

View full text Add to dashboard Cite

The development of insensitivity to guinea pig serum by variant cell lines of Lymphoma 6C3HED has made it possible to examine metabolic features of the lymphoma cells which determine their response, or lack of response, to treatment by guinea pig serum in vivo.Holmquist and Kidd in previous studies from this laboratory showed that such insensitive variants often appeared after treatment of Lymphoma 6C3HED cells in vivo with quantities of guinea pig serum insufficient to bring about complete suppression of their growth (1).The present experiments show that insensitivity to guinea pig serum develops in populations of 6C3HED cells in yet another way, and under conditions in which guinea pig serum is completely absent. It was found that 6C3HED cells could be grown in tissue culture in Eagle's medium. Mter an initial latent period of 2 or more weeks the cultured cells began to proliferate vigorously, but when these cells were implanted into mice, they were found to produce tumors which were completely and permanently insensitive to guinea pig serum. Eagle's medium contains no L-asparagine. When this amino acid was added to the culture medium not only did it provide a strong growth stimulus for cells freshly placed in culture, but the cells retained their original character of sensitivity to guinea pig serum even after prolonged periods of growth.These findings are presented in relation to the direct evidence, given in the companion paper, which indicates that the L-asparaginase of guinea pig serum brings about the inhibition of Lymphoma 6C3HED cells in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS

Broome

1963

The Journal of Experimental Medicine

155

View full text Add to dashboard Cite

show abstract

“…This discovery ushered in a new concept in cancer chemotherapy. Certain cancers, such as acute lymphoblastic leukemia (ALL), 2 do not express the enzyme asparagine synthetase (3,4). As a result, the Asn present in the blood becomes the sole source of this amino acid for ALL cells.…”

mentioning

confidence: 99%

Identification and Structural Analysis of an l-Asparaginase Enzyme from Guinea Pig with Putative Tumor Cell Killing Properties

Schalk

Nguyen

Rigouin

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Guinea pig serum and liver contain an enzyme with L-asparaginase activity. Results: H0W0T5_CAVPO (gpASNase1) displays a low micromolar K m with Asn. Structures of apo and ASP complex are presented. Conclusion: gpASNase1is the likely identity of a guinea pig L-asparaginase endowed with anticancer properties. Significance: The high sequence identity to the human enzymes and its lack of L-glutaminase activity make gpASNase1 a potential replacement for the bacterial enzymes.

show abstract

“…All other experimental tumors inhibited by the enzyme [e.g., Jensen sarcoma (222), Walker 256 carcinosarcoma (221), leukemia L5178Y (223)] also require asparagine for growth in vitro. In each case, sensitivity to the enzyme is lost together with the requirement for exogenous asparagine in nutritional variants of the tumor.…”

Section: Mode Of Actionmentioning

confidence: 99%

L‐Asparaginase: A Review

Wriston¹,

Yellin²

1973

Advances in Enzymology - And Related Areas of Molecular Biology

144

View full text Add to dashboard Cite

Dual Requirement of Walker Carcinosarcoma 256 in vitro for Asparagine and Glutamine

Cited by 95 publications

References 3 publications

EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS

EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS

Identification and Structural Analysis of an l-Asparaginase Enzyme from Guinea Pig with Putative Tumor Cell Killing Properties

L‐Asparaginase: A Review

Contact Info

Product

Resources

About