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0 50 100 150 200 0 50 100 150 ZM) 0 50 100 150 200 T I M E I N M I N U T E S TUMOR DNA CELLS * NORMAL DNA CELLS UNCOUPLED CELLS I'lG. 2. Effect of DNA-ase upon settling rate of coupled and uncoupled cells in presence of rabbit antiserums and normal rabbit serum.substance to stimulate antibody formation or to be detected by precipitin or agglutination reaction. However, it must be admitted that it is difficult at present to understand why a hapten of the complexity of DNA would not precipitate with antiserum. This observation suggests that there might be an insufficient number of specific reactive sites per DNA molecule or that non-specific factors are involved, as observed by Ingraham( 5 ) with respect to non-agglutination of formalin-treated red cells.Summary. Formalinized erythrocytes exposed to purified DNA under the proper conditions can then be specifically agglutinated with antiserum, which has been prepared against the DNA-protein complex. Inhibition of the reaction by homologous DNA is strong evidence that agglutination is an antigen-antibody reaction and not merely a non-specific agglutination. The removal of this reactivity by desoxyribonuclease lends further support to the belief that DNA is functioning as the hapten. Trypsin does not alter the agglutina-tion reaction.