Growth-Promoting Properties of Pyruvate, Oxalacetate, and  -Ketoglutarate For Isolated Walker Carcinosarcoma 256 Cells

Abstract

0 50 100 150 200 0 50 100 150 ZM) 0 50 100 150 200 T I M E I N M I N U T E S TUMOR DNA CELLS * NORMAL DNA CELLS UNCOUPLED CELLS I'lG. 2. Effect of DNA-ase upon settling rate of coupled and uncoupled cells in presence of rabbit antiserums and normal rabbit serum.substance to stimulate antibody formation or to be detected by precipitin or agglutination reaction. However, it must be admitted that it is difficult at present to understand why a hapten of the complexity of DNA would not precipitate with antiserum. This observation suggests that there might be an insufficient number of specific reactive sites per DNA molecule or that non-specific factors are involved, as observed by Ingraham( 5 ) with respect to non-agglutination of formalin-treated red cells.Summary. Formalinized erythrocytes exposed to purified DNA under the proper conditions can then be specifically agglutinated with antiserum, which has been prepared against the DNA-protein complex. Inhibition of the reaction by homologous DNA is strong evidence that agglutination is an antigen-antibody reaction and not merely a non-specific agglutination. The removal of this reactivity by desoxyribonuclease lends further support to the belief that DNA is functioning as the hapten. Trypsin does not alter the agglutina-tion reaction.

Understanding ischemic retinopathies: emerging concepts from oxygen-induced retinopathy

“…Among them, the biological effects of Krebs cycle metabolites, such as succinate, have been scarcely evaluated. Some have been reported to show angiogenic properties in vitro [73][74][75]. It has recently been demonstrated that succinate specifically binds and signals through the G protein-coupled receptor GPR91 [76], but its physiological role remained poorly characterized.…”

Sites of Formation of Immune Globulins and of a Component of C'3

“…Each tube received 2 ml of a medium consisting of Hanks' balanced salt solution to which were added glucose (up to 22 m~), in~sitol (2 rag/ liter), and mixtures of vitamins (25), antibiotics, and amino acids (26) from which either isoleucine or lysine had been omitted. Uniformly labeled C14-L-lysine (605 (/zc/mg) and C14-Lisoleucine (675 #c/rag), obtained from the Institut Pasteur, Paris, were added to a concentration of 2/zc/ml.…”

The Cells of Human Colostrum II. Synthesis of IgA and β1c

“…The culture medium [28] consisted of HBSS plus 0.5% dextrose, 0.5% ovalbumin, 1% BME-vitamins (100 X concentrate), penicillin G (100 U/ml), and an amino acid mixture [13] from which L-lysine and L-isoleucine were omitted. The pH was adjusted to 7.4 with sodium bicarbonate.…”