Dual-Selective Magnetic Analysis of Extracellular Vesicle Glycans

Wang, Zhigang; Sun, Xuecheng; Natalia, Auginia; Tang, Carol; Ang, Christopher Beng Ti; Ong, Chin-Ann Johnny; Teo, Melissa Ching Ching; So, Jimmy Bok Yan

doi:10.1016/j.matt.2019.10.018

Cited by 46 publications

(33 citation statements)

References 52 publications

(67 reference statements)

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“…Using STARs functionalized with antibodies, we developed the assay to achieve direct glycan profiling of cancer-associated EVs in patient specimens. Specifically, we prepared STARs with antibodies against CD24 34 , 35 , a known cancer antigen, to enrich and measure putative tumor-derived EVs. Multiplexed glycan profiling showed highly varied glycan signatures among the clinical samples (Fig.…”

Section: Resultsmentioning

confidence: 99%

Surfactant-guided spatial assembly of nano-architectures for molecular profiling of extracellular vesicles

et al. 2021

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The controlled assembly of nanomaterials into desired architectures presents many opportunities; however, current preparations lack spatial precision and versatility in developing complex nano-architectures. Inspired by the amphiphilic nature of surfactants, we develop a facile approach to guide nanomaterial integration – spatial organization and distribution – in metal-organic frameworks (MOFs). Named surfactant tunable spatial architecture (STAR), the technology leverages the varied interactions of surfactants with nanoparticles and MOF constituents, respectively, to direct nanoparticle arrangement while molding the growing framework. By surfactant matching, the approach achieves not only tunable and precise integration of diverse nanomaterials in different MOF structures, but also fast and aqueous synthesis, in solution and on solid substrates. Employing the approach, we develop a dual-probe STAR that comprises peripheral working probes and central reference probes to achieve differential responsiveness to biomarkers. When applied for the direct profiling of clinical ascites, STAR reveals glycosylation signatures of extracellular vesicles and differentiates cancer patient prognosis.

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Section: Resultsmentioning

confidence: 99%

Surfactant-guided spatial assembly of nano-architectures for molecular profiling of extracellular vesicles

et al. 2021

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“…As demonstrated in various cancer types, the discovery of prognostic gene signatures could provide clinicians with tools for better disease management (52)(53)(54). The potential utility of unique signatures on extracellular vesicles and glycans could also be harnessed for patient prognostication (55). Minimally invasive surgery options in the background of recurrent ovarian cancer treated with HIPEC have recently been suggested as a means of reducing surgical risk and post-operative complications in the subgroup of patients indicated for such surgery, providing desirable outcomes with a reported upside of increased peritoneal uptake of HIPEC (56,57).…”

Section: Discussionmentioning

confidence: 99%

Effect of HIPEC on Peritoneal Recurrence in Peritoneal Metastasis Treated With Cytoreductive Surgery: A Systematic Review

et al. 2021

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BackgroundPeritoneal metastasis (PM) is a late-stage manifestation of intra-abdominal malignancies. The current standard of care indicates that cure can only be achieved with cytoreductive surgery (CRS) which is often indicated with concurrent adjuvant hyperthermic intraperitoneal chemotherapy (HIPEC). However, the utility of HIPEC within subsets of PM is not fully understood. We seek to compare the effectiveness of HIPEC in improving peritoneal recurrence rates in PM of different origins.MethodsWe conducted a systematic review of trials on the PubMed, EMBASE, and Cochrane databases, last searched in August 2021. Biases were assessed using the Cochrane Collaboration’s tool for assessing the risk of bias in randomized trials as well as the Methodological Index for Non-Randomized Studies (MINORS) framework.Results7 gastric PM studies, 3 ovarian PM studies, and 3 colorectal PM studies were included. Recurrence-free survival was improved in the HIPEC + CRS cohort in 5 gastric trials but only 1 ovarian trial and none of colorectal origin.DiscussionOur findings indicate decent effectiveness of HIPEC in gastric PM, but limited utility in ovarian and colorectal PM. Limitations in the current literature are attributed to the paucity of data available, a lack of homogeneity and consideration of novel and personalised treatment regimens. We implore for further studies to be conducted with a focus on patient selection and stratification, and suggest a reframing of approach towards modern molecular and targeted therapeutic options in future studies of HIPEC.Systematic Review Registrationhttps://www.researchregistry.com/browse-the-registry#registryofsystematicreviewsmeta-analyses/registryofsystematicreviewsmeta-analysesdetails/60c1ffff0c1b78001e8efbe3/, identifier reviewregistry1166.

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“…Interestingly, a new integrated analytical platform, termed the integrated magnetic analysis of glycans in extracellular vesicles (iMAGE), was developed to directly analyze the EV glycosylation profile in biological samples. This platform aims to facilitate the EV glycome analysis taking advantage of the magnetic nanotechnologies [ 218 ]. The effectiveness of this strategy was evaluated by spiking kidney and brain cancer-EVs into urine and serum EV-depleted samples, respectively, and analyzing the glycan signatures.…”

Section: Cancer Extracellular Vesicles Glycosylationmentioning

confidence: 99%

“…The distinction of these patients was possible by the increased signal of different lectins associated with a poor prognosis, including the Jacalin, ConA, RCA120, PHA-E, STA, LEL, WGA, DSL and LCA lectins. Although further studies are needed to prove the iMAGE platform’s robustness, this new method of profiling glycans may prove to be very useful in the search for novel biomarkers in cancer research [ 218 ]. Although several techniques can be used to analyze glycans, their study faces several technical challenges.…”

Section: Cancer Extracellular Vesicles Glycosylationmentioning

confidence: 99%

Glycosylation of Cancer Extracellular Vesicles: Capture Strategies, Functional Roles and Potential Clinical Applications

Martins

Ramos

Freitas

et al. 2021

Cells

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Glycans are major constituents of extracellular vesicles (EVs). Alterations in the glycosylation pathway are a common feature of cancer cells, which gives rise to de novo or increased synthesis of particular glycans. Therefore, glycans and glycoproteins have been widely used in the clinic as both stratification and prognosis cancer biomarkers. Interestingly, several of the known tumor-associated glycans have already been identified in cancer EVs, highlighting EV glycosylation as a potential source of circulating cancer biomarkers. These particles are crucial vehicles of cell–cell communication, being able to transfer molecular information and to modulate the recipient cell behavior. The presence of particular glycoconjugates has been described to be important for EV protein sorting, uptake and organ-tropism. Furthermore, specific EV glycans or glycoproteins have been described to be able to distinguish tumor EVs from benign EVs. In this review, the application of EV glycosylation in the development of novel EV detection and capture methodologies is discussed. In addition, we highlight the potential of EV glycosylation in the clinical setting for both cancer biomarker discovery and EV therapeutic delivery strategies.

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Dual-Selective Magnetic Analysis of Extracellular Vesicle Glycans

Cited by 46 publications

References 52 publications

Surfactant-guided spatial assembly of nano-architectures for molecular profiling of extracellular vesicles

Surfactant-guided spatial assembly of nano-architectures for molecular profiling of extracellular vesicles

Effect of HIPEC on Peritoneal Recurrence in Peritoneal Metastasis Treated With Cytoreductive Surgery: A Systematic Review

Glycosylation of Cancer Extracellular Vesicles: Capture Strategies, Functional Roles and Potential Clinical Applications

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