Dual sensitive and rapid detection of glycated human serum albumin using a versatile lead/graphene nanocomposite probe as a fluorescence–electrochemical aptasensor

Putnin, Thitirat; Waiwinya, Wassa; Pimalai, Dechnarong; Chawjiraphan, Wireeya; Sathirapongsasuti, Nuankanya; Japrung, Deanpen

doi:10.1039/d1an00556a

Cited by 12 publications

(11 citation statements)

References 34 publications

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“…Additionally, integrating optical and electrical strategies based on aptamer−protein electrostatic interactions to construct dual-mode detection techniques can further improve the accuracy and sensitivity of biomolecular analysis. 398 As illustrated in Figure 9D, Putnin's group fabricated a composite comprising Pb ions adsorbed on graphene oxide (GO-Pb), serving either as a quencher probe for fluorescence detection or as a signal probe for electrochemical detection. 398 Fluorescence−electrochemical dual detection technique for the quantification of GA was subsequently established by modifying this complex with FAM-labeled GA aptamer.…”

Section: Detection Based On Electrostatic Interactionsmentioning

confidence: 99%

“…398 As illustrated in Figure 9D, Putnin's group fabricated a composite comprising Pb ions adsorbed on graphene oxide (GO-Pb), serving either as a quencher probe for fluorescence detection or as a signal probe for electrochemical detection. 398 Fluorescence−electrochemical dual detection technique for the quantification of GA was subsequently established by modifying this complex with FAM-labeled GA aptamer. When the FAM-aptamer binds to the GO-Pb complex, fluorescence quenching of FAM occurred due to FRET, and the oxidation of Pb via electron transfer at the electrode ceases.…”

Section: Detection Based On Electrostatic Interactionsmentioning

confidence: 99%

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Aptamer–Protein Interactions: From Regulation to Biomolecular Detection

Ji,

Wei,

Zhang

et al. 2023

Chem. Rev.

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Serving as the basis of cell life, interactions between nucleic acids and proteins play essential roles in fundamental cellular processes. Aptamers are unique single-stranded oligonucleotides generated by in vitro evolution methods, possessing the ability to interact with proteins specifically. Altering the structure of aptamers will largely modulate their interactions with proteins and further affect related cellular behaviors. Recently, with the in-depth research of aptamer–protein interactions, the analytical assays based on their interactions have been widely developed and become a powerful tool for biomolecular detection. There are some insightful reviews on aptamers applied in protein detection, while few systematic discussions are from the perspective of regulating aptamer–protein interactions. Herein, we comprehensively introduce the methods for regulating aptamer–protein interactions and elaborate on the detection techniques for analyzing aptamer–protein interactions. Additionally, this review provides a broad summary of analytical assays based on the regulation of aptamer–protein interactions for detecting biomolecules. Finally, we present our perspectives regarding the opportunities and challenges of analytical assays for biological analysis, aiming to provide guidance for disease mechanism research and drug discovery.

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Section: Detection Based On Electrostatic Interactionsmentioning

confidence: 99%

Section: Detection Based On Electrostatic Interactionsmentioning

confidence: 99%

Aptamer–Protein Interactions: From Regulation to Biomolecular Detection

Ji,

Wei,

Zhang

et al. 2023

Chem. Rev.

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“…To date, glycated human serum albumin (GHSA) has been suggested as a new promising diabetes biomarker. − In diabetes patients, the GHSA level can be 2–3-fold higher than that of healthy people. , No fasting is required for GHSA measurement. GHSA was reported to provide more relevant and immediate glucose information relative to HbA1c in children, adolescents, and pregnant women. , Therefore, many attempts have been made to search for GHSA detection strategies. − Previously, liquid chromatography, , affinity chromatography, , colorimetry, Raman spectroscopy, and immunochemistry were studied to detect a GHSA level; however, such methods are costly and time-consuming. More convenient and faster techniques such as a lateral flow assay, an electrochemical immunoassay, specific gel electrophoresis, and optical biosensors ,− are introduced.…”

Section: Introductionmentioning

confidence: 99%

“…Nanomaterials such as metal nanoparticles, graphene, − and graphene quantum dots (GQDs) were used as a substrate and/or a luminophore. Among nanomaterials, zero-dimensional nanosized GQDs have come into focus because of their unique photoluminescence properties, high photostability, non-toxicity, and especially more biocompatibility when compared to other nanosized materials. − The capability to be a good substrate for biomolecules and quenching agents also allows the involvement of GQDs in many disease biosensor platforms, including GHSA detection. ,,− GQDs were used in aptasensors as an aptamer linker and/or a fluorescence quencher. ,,, For fluorescent aptasensors, analyte-selective fluorescent aptamers are first adsorbed onto GQDs in a solution, resulting in fluorescence quenching. The presence of analytes such as albumin induces the release of an aptamer to a target, resulting in fluorescence recovery.…”

Section: Introductionmentioning

confidence: 99%

Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

et al. 2023

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Diabetes mellitus is a chronic metabolic disease involving continued elevated blood glucose levels. It is a leading cause of mortality and reduced life expectancy. Glycated human serum albumin (GHSA) has been reported to be a potential diabetes biomarker. A nanomaterial-based aptasensor is one of the effective techniques to detect GHSA. Graphene quantum dots (GQDs) have been widely used in aptasensors as an aptamer fluorescence quencher due to their high biocompatibility and sensitivity. GHSA-selective fluorescent aptamers are first quenched upon binding to GQDs. The presence of albumin targets results in the release of aptamers to albumin and consequently fluorescence recovery. To date, the molecular details on how GQDs interact with GHSA-selective aptamers and albumin remain limited, especially the interactions of an aptamer-bound GQD (GQDA) with an albumin. Thus, in this work, molecular dynamics simulations were used to reveal the binding mechanism of human serum albumin (HSA) and GHSA to GQDA. The results show the rapid and spontaneous assembly of albumin and GQDA. Multiple sites of albumins can accommodate both aptamers and GQDs. This suggests that the saturation of aptamers on GQDs is required for accurate albumin detection. Guanine and thymine are keys for albumin-aptamer clustering. GHSA gets denatured more than HSA. The presence of bound GQDA on GHSA widens the entrance of drug site I, resulting in the release of open-chain glucose. The insight obtained here will serve as a base for accurate GQD-based aptasensor design and development.

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“…However, owing to issues such as the requirement of operational complexity of the instrumentation and cost, miniaturized sensors that can rapidly detect HbA 1c are urgently needed. Compared with the existing technology, aptamer-based electrochemical biosensors exhibit many advantages including (1) extended stability, (2) minimal blood for testing is required, and (3) using an aptamer [ 8 , 9 , 10 , 11 ] means that cast-type screen printing electrode construction of electrochemical sensors [ 12 , 13 , 14 , 15 ] can be used, which is convenient, enables miniaturization, and is low cost. Testing of HbA 1c is commonly recommended by several countries for monitoring the development of diabetes.…”

Section: Introductionmentioning

confidence: 99%

Ferrocene-Labelled Electroactive Aptamer-Based Sensors (Aptasensors) for Glycated Haemoglobin

Feng

Dou

et al. 2021

Molecules

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Glycated haemoglobin (HbA1c) is a diagnostic biomarker for type 2 diabetes. Traditional analytical methods for haemoglobin (Hb) detection rely on chromatography, which requires significant instrumentation and is labour-intensive; consequently, miniaturized devices that can rapidly sense HbA1c are urgently required. With this research, we report on an aptamer-based sensor (aptasensor) for the rapid and selective electrochemical detection of HbA1c. Aptamers that specifically bind HbA1c and Hb were modified with a sulfhydryl and ferrocene group at the 3′ and 5′-end, respectively. The modified aptamers were coated through sulfhydryl-gold self-assembly onto screen printed electrodes, producing aptasensors with built in electroactivity. When haemoglobin was added to the electrodes, the current intensity of the ferrocene in the sensor system was reduced in a concentration-dependent manner as determined by differential pulse voltammetry. In addition, electrochemical impedance spectroscopy confirmed selective binding of the analytes to the aptamer-coated electrode. This research offers new insight into the development of portable electrochemical sensors for the detection of HbA1c

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Dual sensitive and rapid detection of glycated human serum albumin using a versatile lead/graphene nanocomposite probe as a fluorescence–electrochemical aptasensor

Abstract: Monitoring of glycated human serum albumin (GHSA) as a glycemic marker for screening and monitoring of diabetes mellitus is widely practiced for patients with conditions that affect red blood cell....

Cited by 12 publications

References 34 publications

Aptamer–Protein Interactions: From Regulation to Biomolecular Detection

Aptamer–Protein Interactions: From Regulation to Biomolecular Detection

Binding of Apo and Glycated Human Serum Albumins to an Albumin-Selective Aptamer-Bound Graphene Quantum Dot Complex

Ferrocene-Labelled Electroactive Aptamer-Based Sensors (Aptasensors) for Glycated Haemoglobin

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