Dual signal amplification based on polysaccharide-initiated ring-opening polymerization and click polymerization for exosomes detection

Zhu, Xin; Liu, Zenghui; Li, Jinge; Li, Zutian; Si, Fuchun; Yang, Huaixia; Kong, Jinming

doi:10.1016/j.talanta.2021.122531

Cited by 18 publications

(11 citation statements)

References 45 publications

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“…Other anecdotic or specific synthetic polymers were reported with aptamer, silicone (poly(dimethylsiloxane) (PDMS) (Guo et al., 2021 ), poly(acrylic acid) (PAA), thermosensitive polymers such as poly( N ‐isopropylamide) (PNIPAM), or electroactive polymers including poly[(9,9‐dihexyl‐2,7‐(2‐cyanodivinylene)‐fluorenylenyl‐2,7‐diyl)] (CN‐PDHFV) by Zhu et al. for detection (Zhu et al., 2021 ). All the polymers are electrical insulators except conjugated polymers characterized by the alternating of single and double bonds permitting the delocalisation of pi electrons along the macromolecular structure and consequently the electrical conduction.…”

Section: Discussionmentioning

confidence: 99%

“…BOX 3.1a) as well as thiol‐epoxy (Fig. BOX 3.1e) (Zhu et al., 2021 ). But, the main issue of this reaction is the requirement of an excess of thiol to offset the consumption of thiol in unwanted S‐S bridges.…”

Section: Discussionmentioning

confidence: 99%

“…EVs secreted from lung cancer cells (A4549) (over‐expressing EGFR) were immobilized onto gold chips functionalized with anti‐EGFR mAb to create an electrochemical sensor able to translate the chemical signal into an electrical signal (Figure 2d ) (Zhu et al., 2021 ). For enhanced sensitivity, signal amplification strategy based on polymerization amplification was carried out.…”

Section: Input Of Polymers In Evs Production Enrichment and Detectionmentioning

confidence: 99%

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Exploring the role of polymers to overcome ongoing challenges in the field of extracellular vesicles

Simon,

Lapinte,

Morille

2023

J of Extracellular Vesicle

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Extracellular vesicles (EVs) are naturally occurring nanoparticles released from all eucaryotic and procaryotic cells. While their role was formerly largely underestimated, EVs are now clearly established as key mediators of intercellular communication. Therefore, these vesicles constitute an attractive topic of study for both basic and applied research with great potential, for example, as a new class of biomarkers, as cell‐free therapeutics or as drug delivery systems. However, the complexity and biological origin of EVs sometimes complicate their identification and therapeutic use. Thus, this rapidly expanding research field requires new methods and tools for the production, enrichment, detection, and therapeutic application of EVs. In this review, we have sought to explain how polymer materials actively contributed to overcome some of the limitations associated to EVs. Indeed, thanks to their infinite diversity of composition and properties, polymers can act through a variety of strategies and at different stages of EVs development. Overall, we would like to emphasize the importance of multidisciplinary research involving polymers to address persistent limitations in the field of EVs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Input Of Polymers In Evs Production Enrichment and Detectionmentioning

confidence: 99%

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Exploring the role of polymers to overcome ongoing challenges in the field of extracellular vesicles

Simon,

Lapinte,

Morille

2023

J of Extracellular Vesicle

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“…Strategies of controllable polymerization, such as atom transfer radical polymerization (ATRP) [ 18 , 19 , 20 , 21 , 22 ], reversible addition–fragmentation chain transfer polymerization (RAFT) [ 23 , 24 , 25 , 26 ], ring-opening polymerization (ROP) [ 27 , 28 ], and ring-opening metathesis polymerization (ROMP) [ 29 , 30 , 31 ], are available “grafting-from” methods for surface-initiated polymerization that can be used in the modification of various substrates. Qian Ye et al [ 32 ] introduced a novel surface-initiated ring-opening metathesis polymerization (SI-ROMP) to prepare high-thickness zwitterionic polymer brushes functionalized through the use of imidazolium salts.…”

Section: Introductionmentioning

confidence: 99%

A Versatile Approach for the Synthesis of Antimicrobial Polymer Brushes on Natural Rubber/Graphene Oxide Composite Films via Surface-Initiated Atom-Transfer Radical Polymerization

Zhu,

Li,

Liu

et al. 2024

Molecules

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A simple strategy was adopted for the preparation of an antimicrobial natural rubber/graphene oxide (NR/GO) composite film modified through the use of zwitterionic polymer brushes. An NR/GO composite film with antibacterial properties was prepared using a water-based solution-casting method. The composited GO was dispersed uniformly in the NR matrix and compensated for mechanical loss in the process of modification. Based on the high bromination activity of α–H in the structure of cis-polyisoprene, the composite films were brominated on the surface through the use of N-bromosuccinimide (NBS) under the irradiation of a 40 W tungsten lamp. Polymerization was carried out on the brominated films using sulfobetaine methacrylate (SBMA) as a monomer via surface-initiated atom transfer radical polymerization (SI-ATRP). The NR/GO composite films modified using polymer brushes (PSBMAs) exhibited 99.99% antimicrobial activity for resistance to Escherichia coli and Staphylococcus aureus. A novel polymer modification strategy for NR composite materials was established effectively, and the enhanced antimicrobial properties expand the application prospects in the medical field.

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“…A turnaround took place in 2002 when the Cu(I)-catalyzed azide–alkyne cycloaddition (CuAAC) was reported independently by Sharpless et al and Meldal et al , As a typical click reaction, CuAAC could produce sole 1,4-disubstituted 1,2,3-triazoles in excellent yields under mild conditions, which has been developed into a useful polymerization technique, i.e., Cu(I)-catalyzed azide–alkyne click polymerization (CuAACP) . Thanks to its remarkable click features, CuAACP has been widely used for the preparation of 1,4-regioregular polytriazoles (PTAs) with advanced structures and desired functional properties, which could serve as biomaterials, photoelectric materials, nonlinear optical materials, shape memory materials, self-healing materials, − and so on. To avoid the detriment of the residue metallic species to the optoelectronic and bioapplication of the product, metal-free click polymerization (MFCP) of azides and alkynes was also reported by our groups for the preparation of 1,4-regioregular PTAs …”

Section: Introductionmentioning

confidence: 99%

Nickel-Catalyzed Azide–Alkyne Click Polymerization toward 1,5-Regioregular Polytriazoles

Huang,

Liu,

Qin

et al. 2023

Macromolecules

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Dual signal amplification based on polysaccharide-initiated ring-opening polymerization and click polymerization for exosomes detection

Cited by 18 publications

References 45 publications

Exploring the role of polymers to overcome ongoing challenges in the field of extracellular vesicles

Exploring the role of polymers to overcome ongoing challenges in the field of extracellular vesicles

A Versatile Approach for the Synthesis of Antimicrobial Polymer Brushes on Natural Rubber/Graphene Oxide Composite Films via Surface-Initiated Atom-Transfer Radical Polymerization

Nickel-Catalyzed Azide–Alkyne Click Polymerization toward 1,5-Regioregular Polytriazoles

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