Dual-targeted peptide-conjugated multifunctional fluorescent probe with AIEgen for efficient nucleus-specific imaging and long-term tracing of cancer cells

Cheng, Yong; Sun, Chunli; Ou, Xiaowen; Liu, Bi‐Feng; Lou, Xiaoding; Xia, Fan

doi:10.1039/c7sc00402h

Cited by 109 publications

(76 citation statements)

References 80 publications

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“…Similarly, by using the corresponding peptides as targeting ligands, selective fluorogenic visualization was achieved for many proteins in cells, such as LAPTM4B (Table , entry 6), endogenous Mdm2 receptors (Table , entry 7), ER proteins (Table , entry 8), IL‐1 (Table , entry 9), TIP‐1 (Table , entry 10), TfR (Table , entries 11–12), and transmembrane receptor tyrosine kinase EphA2 (Table , entry 13) . Employing a dual‐targeted peptide‐conjugated fluorescent probe to light up integrin on the membrane and CD13 biomarkers enabled the monitoring of a complicated intracellular trafficking process on a large spatiotemporal scale (Table , entry 5), including internalization, transportation, and penetration into the nucleus region, which was indicative of a high degree of versatility of AIE methods to track intracellular molecular events . The real‐time quantification of proteins in vitro and in cells enabled an efficient screening of drug candidates .…”

Section: Detection Localization and Quantification Of Proteinsmentioning

confidence: 99%

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Xie

Wong

Chen

et al. 2019

Chemistry A European J

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Protein is one of the four most important biomacromolecules in living systems. The detection, quantification, localization, and characterization of proteins is essential for an understanding of biological fundamentals, as well as for the diagnostics and treatment of protein‐related diseases. By using intrinsic and extrinsic fluorescence, different techniques have been established to study proteins, many of which are now being routinely used in research laboratories and clinics. This review summarizes the applications of aggregation‐induced emission (AIE) fluorescence in protein science. In contrast to traditional fluorescent dyes, the activation of AIE dyes is mainly attributed to the restriction of intramolecular motions. This unique turn‐on mechanism of AIE dyes allows researchers to develop novel fluorogenic strategies for sensitive, selective, and reliable analysis of proteins. This review focuses on introducing AIE strategies for 1) detection, localization, and quantification of proteins; 2) probing polymer conformational transitions of proteins; 3) characterization of protein–ligand interactions; and 4) evaluation of enzyme activities. Perspectives and challenges with respect to this emerging field of protein characterization are offered.

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Section: Detection Localization and Quantification Of Proteinsmentioning

confidence: 99%

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Xie

Wong

Chen

et al. 2019

Chemistry A European J

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“…For examples, some diseases were caused by protein aggregation. Xia and co‐workers developed peptide multifunctionalized AIEgen TCNTP for bioimaging applications. TCNTP was synthesized via an AIEgen TPE‐Py conjugating to a dual‐targeted peptide (cNGR‐CPP‐NLS‐RGD).…”

Section: Key Application In Biomedicinementioning

confidence: 99%

“…B) The chemical conjugate structure of peptide cNGR‐CPP‐NLS‐RGD‐functionalized PyTPE TNCP and its fluorescence imaging in living cells. Adapted with permission . Copyright 2017, The Royal Society of Chemistry.…”

Section: Key Application In Biomedicinementioning

confidence: 99%

Biomacromolecule‐Functionalized AIEgens for Advanced Biomedical Studies

Duan

et al. 2019

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The advances in bioinformatics and biomedicine have promoted the development of biomedical imaging and theranostic systems to respectively extend the endogenous biomarker imaging with high contrast and enhance the therapeutic effect with high efficiency. The emergence of biomacromolecule‐functionalized aggregation‐induced emitters (AIEgens), utilizing AIEgens, and biomacromolecules (nucleic acids, peptides, glycans, and lipids), displays specific targeting ability to cancer cell, improved biocompatibility, reduced toxicity, enhanced therapeutic effect, and so forth. This review summarizes the rational design of biomacromolecule‐functionalized AIEgens and their biomedical applications in recent ten years, including high‐resolution optical imaging of cell, tissue, and small animal model with low background; the biomarker detection for early diagnosis and prognosis; the delivery and monitoring of prodrugs; image‐guide photodynamic therapy and its combination with chemotherapy. Through illustrating their functional mechanisms and application, it is hoped that this review would open up a completely new train of research thought for attracted researchers in various fields.

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“…The notoriousA CQ effect has limited various fluorescentp robest owardu se in imaging and analyte detectioni np ractical applications.H ence, the design of fluorescent sensors that are not subject to the ACQ effect is indispensable.I nc ontrast, fluorogens that exhibit aggregation-induced emission have captured tremendous attention in recent years. [9][10][11][12] The mechanisms of AIEgensh ave been investigated because intramolecular motions (RIM) were restricted. Their unique fluorescencep roperty is that there is almost no fluorescence in solution.…”

Section: Introductionmentioning

confidence: 99%

AIEgens/Nucleic Acid Nanostructures for Bioanalytical Applications

Wang

Huang

et al. 2019

Chemistry An Asian Journal

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DNA occupies significant roles in life processes, which include encoding the sequences of proteins and accurately transferring genetic information from generation to generation. Recent discoveries have demonstrated that a variety of biological functions are correlated with DNA′s conformational transitions. The non‐B form has attained great attention among the diverse forms of DNA over the past several years. The main reason for this is that a large number of studies have shown that the non‐B form of DNA is associated with gross deletions, inversions, duplications, translocations as well as simple repeating sequences, which therefore causes human diseases. Consequently, the conformational transition of DNA between the B‐form and the non‐B form is important for biology. Conventional fluorescence probes based on the conformational transitions of DNA usually need a fluorophore and a quencher group, which suffers from the complex design of the structure and tedious synthetic procedures. Moreover, conventional fluorescence probes are subject to the aggregation‐caused quenching (ACQ) effect, which limits their application toward imaging and analyte detection. Fluorogens exhibiting aggregation‐induced emission (AIE) have attracted tremendous attention over the past decade. By taking advantage of this unique behavior, plenty of fluorescent switch‐on probes without the incorporation of fluorescent quenchers/fluorophore pairs have been widely developed as biosensors for imaging a variety of analytes. Herein, the recent progress in bioanalytical applications on the basis of aggregation‐induced emission luminogens (AIEgens)/nucleic acid nanostructures are presented and discussed.

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Dual-targeted peptide-conjugated multifunctional fluorescent probe with AIEgen for efficient nucleus-specific imaging and long-term tracing of cancer cells

Abstract: Precisely targeted transportation of a long-term tracing regent to a nucleus with low toxicity is one of the most challenging concerns in revealing cancer cell behaviors.

Cited by 109 publications

References 80 publications

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Biomacromolecule‐Functionalized AIEgens for Advanced Biomedical Studies

AIEgens/Nucleic Acid Nanostructures for Bioanalytical Applications

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