Dual thermo- and pH-responsive poly(N-isopropylacrylamide-co-(2-dimethylamino) ethyl methacrylate)-g-PEG nanoparticle system and its potential in controlled drug release

Najafipour, Aylar; Mahdavian, Ali Reza; Sadeghi‐Aliabadi, Hojjat; Fassihi, Afshin

doi:10.1007/s00289-019-02895-9

Cited by 15 publications

(5 citation statements)

References 19 publications

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“…However, it is desirable to increase the LCST of PNIPAM to body temperature (around 37 °C) for in vivo tests. To achieve this issue, copolymerization of NIPAM with other hydrophilic monomers such as acrylic acid or DEAEMA is suggested. − …”

Section: Introductionmentioning

confidence: 99%

MTX-Loaded Dual Thermoresponsive and pH-Responsive Magnetic Hydrogel Nanocomposite Particles for Combined Controlled Drug Delivery and Hyperthermia Therapy of Cancer

et al. 2020

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In recent years, the exploitation of magnetic nanoparticles in smart polymeric matrices have received increased attention in several fields as site-specific drug delivery systems. Here, ultrasonic-assisted emulsion copolymerization of N-isopropylacrylamide (NIPAM) and 2-(N,N-diethylaminoethyl) methacrylate (DEAEMA) in the presence of Fe3O4 nanoparticles was employed to prepare pH- and temperature-responsive magnetite nanocomposite particles (MNCPs). The obtained MNCPs were fully characterized by TEM, DSC, FT-IR, VSM, and XRD techniques. They had an average particle size of 70 nm with a lower critical solution temperature of 42 °C and superparamagnetic properties. In addition, MNCPs were loaded with methotrexate (MTX) as an anticancer drug, and their in vitro drug release was studied in different pH values and temperatures and in the presence of an alternating magnetic field. Noteworthy that the highest rate of MTX release was observed at pH 5.5 and 42 °C. Cell viability of the treated MCF-7 human breast cancer cell line with free MTX, MNCPs, and MTX-loaded MNCPs or in combination with magnetic hyperthermia (MHT) and water-based hyperthermia was comparatively studied. The obtained results showed about 17% higher antiproliferative activity for the MTX-loaded MNCPs accompanied by MHT relative to that of free MTX.

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Section: Introductionmentioning

confidence: 99%

MTX-Loaded Dual Thermoresponsive and pH-Responsive Magnetic Hydrogel Nanocomposite Particles for Combined Controlled Drug Delivery and Hyperthermia Therapy of Cancer

et al. 2020

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“…It is worth noting that the liposome particle size seen under TEM was about 100nm, which was signi cantly smaller than the data measured by DLS, this experiment was done in triplicate and of different concentration to avoid the in uence of concentration, this phenomena might be attributed to PEGylation [33,46], PEG was not successfully dyed by phosphotungstic acid, therefore it could not be observed under TEM.…”

Section: Resultsmentioning

confidence: 81%

Characterization and Anticancer Effects of Folate Targeted Inotodiol Liposome From Inonotus Obliquus (Chaga Mushroom)

Gao

Chen

et al. 2021

Preprint

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Inotodiol, one tetracyclic triterpenoid isolated from inonotus obliquus (Chaga mushroom) possess excellent anticancer, antioxidant and anti-inflammatory activities, however it has not been applied to medical use due to its low solubility and low bioavailability. Liposome as a good nanodrug carrier with EPR and good biocompatibility has received much research attention. To improve the solubility and bioavailability of IOP, we prepared inotodiol liposomes (IOP-Lps) and folic-acid targeting IOP liposome (FA-IOP-Lps) by ultrasonic method, and their particle size, morphology, zeta potential, entrapment efficiency (EE) and drug loading rate (DL) were characterized by DLS, TEM, FT-IR and HPLC respectively. Their in vitro cytotoxicity of human cervical cancer cells HeLa, human liver cancer cells HepG2 and human breast cancer cells MCF-7 were assessed using the MTT assay. The results showed that IOP-Lps and FA-IOP-Lps possess significant anticancer effects. The results of TEM, FT-IR and DLS confirmed the formation of liposome. The particle size of IOP-Lps were 201.07±6.47 nm and ζ-potential of -50.2±0.5 mV, FA-IOP-Lps 224.33±1.86 nm and ζ-potential of -51.2±0.3 mV, the EE of IOP-Lps and FA-IOP-Lps were 79.14% and 77.33%, respectively. IOP-Lps and FA-IOP-Lps could selectively kill HeLa, HepG2 and MCF-7 cancer cells while nontoxic to normal L02 cells. This is the first study to give out in vitro information of inotodiol’ s anticancer effects based on nanocarriers. Besides, this drug delivery system is of good sustained release and targeted delivery effects to promote the utilization of both inotodiol and other natural hydrophobic compounds in target treatment of cancers.

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“…By increasing the temperature from 25 to 37 °C, the release rate of both 5‐FU and MTX increases significantly and tends to equilibrium in a shorter time. Above the VPTT, the PNIPAM chains collapse, [ 39 ] and the shrinkage of the nanoparticles leads to the rupture of the hydrogen bonds formed by the drug molecules and the polymer matrix, resulting in a higher cumulative release ratio of drugs.…”

Section: Resultsmentioning

confidence: 99%

Preparation of PMMA‐Based Temperature/pH Responsive Nanoparticles Encapsulating 5‐Fluorouracil and Methotrexate In Situ by One‐Pot Dispersion Photopolymerization

Yu,

Shao,

et al. 2024

Macromolecular Bioscience

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In order to achieve long‐term and controllable release of anti‐tumor drugs at specific sites, temperature/pH responsive nanoparticles encapsulating 5‐fluorouracil (5‐FU) and methotrexate (MTX) in situ were prepared through dispersion photopolymerization under green LED irradiation. The physicochemical properties of nanoparticles were characterized by SEM, FTIR, DLS, TG/DSC and XRD. In vitro drug release at different temperatures and pH values was examined to ascertain the release pattern of two drugs, which can be well described by Korsmeyer‐Peppas kinetic model. The cytotoxicity evaluation illustrated that the tumor cells could be more effectively killed by the drug‐loaded nanoparticles, and the improved therapeutic effect was attributed to the controllable and sustainable drug release as well as the enhanced cellular uptake. The blood safety and good biocompatibility of nanoparticles were further confirmed by hemolysis assay, indicating the prepared nanoparticles were potential candidates for effective tumor treatment.This article is protected by copyright. All rights reserved

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Dual thermo- and pH-responsive poly(N-isopropylacrylamide-co-(2-dimethylamino) ethyl methacrylate)-g-PEG nanoparticle system and its potential in controlled drug release

Cited by 15 publications

References 19 publications

MTX-Loaded Dual Thermoresponsive and pH-Responsive Magnetic Hydrogel Nanocomposite Particles for Combined Controlled Drug Delivery and Hyperthermia Therapy of Cancer

MTX-Loaded Dual Thermoresponsive and pH-Responsive Magnetic Hydrogel Nanocomposite Particles for Combined Controlled Drug Delivery and Hyperthermia Therapy of Cancer

Characterization and Anticancer Effects of Folate Targeted Inotodiol Liposome From Inonotus Obliquus (Chaga Mushroom)

Preparation of PMMA‐Based Temperature/pH Responsive Nanoparticles Encapsulating 5‐Fluorouracil and Methotrexate In Situ by One‐Pot Dispersion Photopolymerization

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