Dual window method for processing spectroscopic optical coherence tomography signals with simultaneously high spectral and temporal resolution

Robles, Francisco E.; Graf, Robert N.; Wax, Adam

doi:10.1364/oe.17.006799

Cited by 88 publications

(86 citation statements)

References 22 publications

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“…Light scattering spectroscopy (LSS) was incorporated in OCT for depth resolved nuclear morphology measurements 11,12 . Spectroscopic information was extracted from OCT data 13 , angular resolved low coherence interferometry was demonstrated for the assessment of subcellular features 14 , and molecular imaging was realized 15 . Interferometric synthetic aperture microscopy (ISAM) has been developed to improve the out of focal plane resolution 16 .…”

Section: Nano-sensitive Optical Coherence Tomographymentioning

confidence: 99%

Nano-sensitive optical coherence tomography

et al. 2014

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Section: Nano-sensitive Optical Coherence Tomographymentioning

confidence: 99%

Nano-sensitive optical coherence tomography

et al. 2014

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“…A recently developed frequency transform approach, called the dual-window method, simultaneously utilizes a broad and narrow transformation window in order to obtain high resolution spectroscopy measurements without any tradeoff in depth resolution. 34 The dual window (DW) method was implemented for fLCI and accurately characterized phantoms containing micrometer-sized particles 35 and tissue samples. 36 Figure 7 illustrates the added contrast obtained with spectral analysis using the DW method, comparing a standard OCT image of the microsphere phantom composed of two different sizes with the DW method applied to fLCI (Fig.…”

Section: Light Scattering From Cells and Subcellular Structuresmentioning

confidence: 99%

Spectroscopy of Scattered Light for the Characterization of Micro and Nanoscale Objects in Biology and Medicine

et al. 2014

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The biomedical uses for the spectroscopy of scattered light by micro and nanoscale objects can broadly be classified into two areas. The first, often called light scattering spectroscopy (LSS), deals with light scattered by dielectric particles, such as cellular and sub-cellular organelles, and is employed to measure their size or other physical characteristics. Examples include the use of LSS to measure the size distributions of nuclei or mitochondria. The native contrast that is achieved with LSS can serve as a non-invasive diagnostic and scientific tool. The other area for the use of the spectroscopy of scattered light in biology and medicine involves using conducting metal nanoparticles to obtain either contrast or electric field enhancement through the effect of the surface plasmon resonance (SPR). Gold and silver metal nanoparticles are non-toxic, they do not photobleach, are relatively inexpensive, are wavelength-tunable, and can be labeled with antibodies. This makes them very promising candidates for spectrally encoded molecular imaging. Metal nanoparticles can also serve as electric field enhancers of Raman signals. Surface enhanced Raman spectroscopy (SERS) is a powerful method for detecting and identifying molecules down to single molecule concentrations. In this review, we will concentrate on the common physical principles, which allow one to understand these apparently different areas using similar physical and mathematical approaches. We will also describe the major advancements in each of these areas, as well as some of the exciting recent developments.Index headings: Light scattering; Spectroscopy; Nanoparticle; Surface plasmon; Surface enhanced Raman; SERS. INTRODUCTIONT he spectroscopy of scattered light has played an increasingly important role in biology and medicine because it can provide information about subcellular and extracellular biological structures using native contrast, as well as information about nanoparticles that are employed as the exogenous imaging markers or vehicles for the delivery of therapeutic agents. Light scattering by these microscopic objects is governed by the very basic principles of electromagnetic wave propagation and interaction with matter developed many decades ago. The impressive advances in biomedical instrumentation that have recently been achieved are examples of the successful applications of these fundamental principles. An analogy can be made between the development of biomedical light scattering and the development of other medical imaging modalities, for example the discovery of the very basic physics of X-rays led to the development of computed tomography decades later, while the initial understanding of another basic physical effect, nuclear magnetic resonance, eventually led to magnetic resonance imaging. Thus it can be argued that the field of biomedical optics is primarily concerned with the technological development of new medical applications of electromagnetic wave interactions, described by Maxwell's equations, with exogenous and endogenous...

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“…Light scattering spectroscopy has been used in OCT to study the three-dimensional morphology of the cell nuclei [17,18]. Spectral characteristics of the objects were derived from OCT [19], and the authors of [20] studied molecules. A correlation method for imaging the vascular system has been recently developed at our laboratory [21].…”

Section: Introductionmentioning

confidence: 99%

Nanosensitive optical coherence tomography for the study of changes in static and dynamic structures

Alexandrov¹,

Subhash²,

Leahy³

2014

Quantum Electron.

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Publication InformationS. Alexandrov, H. Subhash, M. Leahy (2014) 'Nanosensitive optical coherence tomography for the study of changes in static and dynamic structures'. Quantum Electronics, 44 (7):657-663. Publisher IOP ScienceLink to publisher's version http://dx.doi.org/10.1070/QE2014v044n07ABEH015487Item record http://hdl.handle.net/10379/4532Quantum Electronics 44 (7) 657 - 663 (2014) © 2014 Kvantovaya Elektronika and Turpion Ltd Abstract. We briefly discuss the principle of image formation in Fourier domain optical coherence tomography (OCT). The theory of a new approach to improve dramatically the sensitivity of conventional OCT is described. The approach is based on spectral encoding of spatial frequency. Information about the spatial structure is directly translated from the Fourier domain to the image domain as different wavelengths, without compromising the accuracy. Axial spatial period profiles of the structure are reconstructed for any volume of interest within the 3D OCT image with nanoscale sensitivity. An example of application of the nanoscale OCT to probe the internal structure of medico-biological objects, the anterior chamber of an ex vivo rat eye, is demonstrated.

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Dual window method for processing spectroscopic optical coherence tomography signals with simultaneously high spectral and temporal resolution

Cited by 88 publications

References 22 publications

Nano-sensitive optical coherence tomography

Nano-sensitive optical coherence tomography

Spectroscopy of Scattered Light for the Characterization of Micro and Nanoscale Objects in Biology and Medicine

Nanosensitive optical coherence tomography for the study of changes in static and dynamic structures

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