Duguetia pycnastera Sandwith (Annonaceae) Leaf Essential Oil Inhibits HepG2 Cell Growth In Vitro and In Vivo

Abstract

Duguetia pycnastera Sandwith (Annonaceae) is a tropical tree that can be found in the Guyanas, Bolivia, Venezuela, and Brazil. In Brazil, it is popularly known as “ata”, “envira”, “envira-preta”, and “envira-surucucu”. In the present work, we investigated the in vitro and in vivo HepG2 cell growth inhibition capacity of D. pycnastera leaf essential oil (EO). The chemical composition of the EO was determined by GC–MS and GC–FID analyses. The alamar blue assay was used to examine the in vitro cytotoxicity of EO in cancer cell lines and non-cancerous cells. In EO-treated HepG2 cells, DNA fragmentation was measured by flow cytometry. The in vivo antitumor activity of the EO was assessed in C.B-17 SCID mice xenografted with HepG2 cells treated with the EO at a dosage of 40 mg/kg. Chemical composition analysis displayed the sesquiterpenes α-gurjunene (26.83%), bicyclogermacrene (24.90%), germacrene D (15.35%), and spathulenol (12.97%) as the main EO constituents. The EO exhibited cytotoxicity, with IC50 values ranging from 3.28 to 39.39 μg/mL in the cancer cell lines SCC4 and CAL27, respectively. The cytotoxic activity of the EO in non-cancerous cells revealed IC50 values of 16.57, 21.28, and >50 μg/mL for MRC-5, PBMC, and BJ cells, respectively. An increase of the fragmented DNA content was observed in EO-treated HepG2 cells. In vivo, EO displayed tumor mass inhibition activity by 47.76%. These findings imply that D. pycnastera leaf EO may have anti-liver cancer properties.

An integrated approach utilizing raman spectroscopy and chemometrics for authentication and detection of adulteration of agarwood essential oils

“…The 26 components identified are numbered in the figure, which matches the serial numbers of each component in Table 2. (Jerković et al, 2011;Su et al, 2012;Chen et al, 2017;Han et al, 2019;Heidary Jamebozorgi et al, 2019;Ben Hassine et al, 2021;Camele et al, 2021;Geng, 2021;Li et al, 2021;Abu-Darwish et al, 2022;Chen et al, 2022;Costa et al, 2022;Jaradat et al, 2022;Yao et al, 2022).…”

“… RI a , calculated from R T s, in relation to those of a series C 7 -C 40 of n-alkanes. RI b , from the literatures ( Jerković et al, 2011 ; Su et al, 2012 ; Chen et al, 2017 ; Han et al, 2019 ; Heidary Jamebozorgi et al, 2019 ; Ben Hassine et al, 2021 ; Camele et al, 2021 ; Geng, 2021 ; Li et al, 2021 ; Abu-Darwish et al, 2022 ; Chen et al, 2022 ; Costa et al, 2022 ; Jaradat et al, 2022 ; Yao et al, 2022 ). …”

Chemical Composition, In Vitro Antitumor Effect, and Toxicity in Zebrafish of the Essential Oil from Conyza bonariensis (L.) Cronquist (Asteraceae)

“…Conventional chemotherapy is not selective [90], and thus, damage can also occur in healthy cells, such as blood cells [91][92][93][94]. EOs composed mostly of monoterpenes and sesquiterpenes show cytotoxicity against PBMCs, such as EO from Duguetia pycnastera leaves (IC 50 : 21.28 µg/mL) [95], EO from Satureja khuzistanica aerial parts (IC 50 : 28.21 µg/mL) [96], and EO from Xylopia laevigata leaves (IC 50 : 35.30 µg/mL) [97]. Thus, the greater cytotoxic effect of CBEO on PBMC (IC 50 : 2.68 µg/mL) may be related to the major compound, (Z)-2-lachnophyllum ester, which has no report in the literature.…”

Therapeutic Effects of Natural Products on Liver Cancer and Their Potential Mechanisms

“…In vitro and in vivo studies indicate that essential oil extracted from Artemisia argyi downregulates the mRNA and protein expression of DEPDC1, weakens Wnt/β-catenin signaling by reducing the production of Wnt1 and β-catenin, and prevents epithelial–mesenchymal transition (EMT) by downregulating vimentin and upregulating E-cadherin [ 118 ]. Additionally, essential oils extracted from the leaves of Conobea scoparioides Benth, Duguetia pycnastera Sandwith, and the bark of Aniba parviflora Mez inhibit the proliferation of HepG2 cells and suppress the growth of xenograft tumors in mice [ 119 , 120 , 121 ]. Pogostemon cablin essential oils, essential oil of lemon myrtle, and Cyperus articulatus L. rhizome essential oil exert anti-tumor effects by blocking the cell cycle and inducing apoptosis in liver cancer cells [ 122 , 123 ].…”