Dulcitol-malonate-phenylalanine agar for the identification of Salmonella and other Enterobacteriaceae

Abstract

An agar medium combining dulcitol fermentation, malonate utilization, and phenylalanine deamination was evaluated with 229 isolates representing 19 genera. All reactions agreed with those obtained on conventional media.

o-Nitrophenyl-beta-D-galactopyranoside-urease-indole broth, a new composite tube medium for Salmonella screening

“…Numerous composite media aimed at combining different biochemical reactions into a one-tube system have been developed and are used in microbiological laboratories (1,3,8,10).…”

“…Strain identification was done by methods described in Bergey's Manual of Systematic Bacteriology (2), with reference to newer reports for the new species (4,6,7). The strains included 2 Cedecea isolates, 56 Citrobacter isolates, 59 Enterobacter isolates, 124 Escherichia isolates, 54 Hafnia isolates, 45 Klebsiella isolates, 1 Kluyvera isolate, 273 Morganella isolates, 1,273 Proteus isolates, 224 Providencia isolates, 224 Salmonella isolates (220 from subgroup I and 4 from subgroup III), 69 Serratia isolates, 3 Shigella isolates, and S Yersinia isolates. p-GUI medium was prepared by the following formula: Trypticase peptone (BBL Microbiology Systems, Cockeysville, Md.…”