Salmonella

Andrews, Wallace H; Flowers, Russell S; Silliker, J. H.; Bailey, J. S.

doi:10.2105/9780875531755ch37

Cited by 36 publications

(24 citation statements)

References 68 publications

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“…To improve the Salmonella isolation from environmental water, we combined our newly developed Salmonella detection quantitative PCR (qPCR) assay with sample concentration and enrichment and the BAM Salmonella isolation method (29) and compared the efficiencies between the two protocols. Two parallel sets of experiments (A and B) were performed.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, the aim of this study was to investigate the prevalence, diversity, and antimicrobial resistance of Salmonella enterica in irrigation pond water in produce farms in the southeastern United States. During the course of the study, we also developed an improved and efficient Salmonella isolation method and compared it to the traditional method in the U.S. FDA's Bacteriological Analytical Manual (29).…”

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confidence: 99%

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Diversity and Antimicrobial Resistance of Salmonella enterica Isolates from Surface Water in Southeastern United States

Vellidis

Liu

et al. 2014

Appl Environ Microbiol

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A study of prevalence, diversity, and antimicrobial resistance of Salmonella enterica in surface water in the southeastern United States was conducted. A new scheme was developed for recovery of Salmonella from irrigation pond water and compared with the FDA's Bacteriological Analytical Manual (8th ed., 2014) (BAM) method. Fifty-one isolates were recovered from 10 irrigation ponds in produce farms over a 2-year period; nine Salmonella serovars were identified by pulsed-field gel electrophoresis analysis, and the major serovar was Salmonella enterica serovar Newport (S. Newport, n ‫؍‬ 29), followed by S. enterica serovar Enteritidis (n ‫؍‬ 6), S. enterica serovar Muenchen (n ‫؍‬ 4), S. enterica serovar Javiana (n ‫؍‬ 3), S. enterica serovar Thompson (n ‫؍‬ 2), and other serovars. It is noteworthy that the PulseNet patterns of some of the isolates were identical to those of the strains that were associated with the S. Thompson outbreaks in 2010, 2012, and 2013, S. Enteritidis outbreaks in 2011 and 2013, and an S. Javiana outbreak in 2012. Antimicrobial susceptibility testing confirmed 16 S. Newport isolates of the multidrug resistant-AmpC (MDR-AmpC) phenotype, which exhibited resistance to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline (ACSSuT), and to the 1st, 2nd, and 3rd generations of cephalosporins (cephalothin, amoxicillin-clavulanic acid, and ceftriaxone). Moreover, the S. Newport MDR-AmpC isolates had a PFGE pattern indistinguishable from the patterns of the isolates from clinical settings. These findings suggest that the irrigation water may be a potential source of contamination of Salmonella in fresh produce. The new Salmonella isolation scheme significantly increased recovery efficiency from 21.2 (36/170) to 29.4% (50/170) (P ‫؍‬ 0.0002) and streamlined the turnaround time from 5 to 9 days with the BAM method to 4 days and thus may facilitate microbiological analysis of environmental water.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Diversity and Antimicrobial Resistance of Salmonella enterica Isolates from Surface Water in Southeastern United States

Vellidis

Liu

et al. 2014

Appl Environ Microbiol

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“…Analyses were done using the most probable number (MPN) method according Kornacki & Johnson (2001). To quantify Salmonella sp., samples of each treatment of fruit salad (25 g) were homogenized in 225 mL of buffered peptone water, according to Andrews et al (2001). Psychrotrophic bacteria were enumerated according to Cousin et al (2001) with Plate Count Agar (PCA).…”

Section: Microbiological Analysismentioning

confidence: 99%

Fruit salad as a new vehicle for probiotic bacteria

et al. 2016

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This work aimed to study the use of fruit salads as carriers for Lactobacillus rhamnosus HN001. We evaluated the viability of this probiotic in fruit salads and the phsyico-chemical, microbiological and sensory properties of this food. Scanning electron microscopy (SEM) was used to verify microorganism adhesion on the fruit tissues. The viability of L. rhamnosus in fruit salads was 8.49 log CFU . g -1 after 120 hours. SEM images showed that fruit tissue provided protection for probiotic. Adhesion sites were observed in higher quantity in banana, apple and guava. The addition of L. rhamnosus did not alter texture of fruits (p > 0.05). Fruit salads containing probiotic had different values of pH and acidity compared to the control (p < 0.05). Ascorbic acid content decreased over time; however, total carotenoids did not significantly decrease (p > 0.05). Fruit salads containing L. rhamnosus showed counts of psychotrophic microorganisms of at least 2.0 log CFU . g -1 lower than control salad after 120 h of refrigerated storage. The fruit salad was well accepted by consumers. Therefore, this product can be used as a carrier for probiotic and an alternative to consuming functional foods.Keywords: acceptability; fruit mix; microscopy; probiotic carrier.Practical Application: Fruit salads can be used as a carrier for probiotic bacteria.

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“…Plates were incubated at 35 Ϯ 2°C for 24 Ϯ 2 h. On day 4, plates were examined for the presence of typical Salmonella colonies. Typical colonies were confirmed as Salmonella as specified in the BAM (2).…”

mentioning

confidence: 99%