This study provides a reconstruction of the 1.5 ka palaeoenvironmental evolution of the small, and presently dry, Milna valley on the island of Vis. Sediments from the valley were studied using multi-proxy approach, applying sedimentological, mineralogical, petrological, anthracological, malacological, 14 C and land cover analyses, in regard to climatic, vegetation, and hydrological changes. The mineral composition of the siliciclastic detritus from the Milna valley points to the Dinaride Ophiolite Zone in Bosnia as its dominant source of origin, eroded by the Neretva River, and deposited in the form of alluvial sediments in the Central Adriatic area. However, Cetina and Drin Rivers may have also contributed some of the sediments. Regional aeolian resedimentation of the material occurred during Pleistocene, which later accumulated and became preserved in the Milna valley. Beside the Dinaride Ophiolite Zone, part of the mineral composition is influenced by minerals from the Alpine region and by neutral to basic volcanism, probably of Italian origin.