Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats

El-Baz, Farouk; Salama, Abeer; Hussein, Rehab A.

doi:10.1016/j.toxrep.2019.10.017

Cited by 33 publications

(24 citation statements)

References 49 publications

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“…D . salina , in another study, regulates oxidative stress and protects the liver from fibrosis [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have proven the antioxidant and anti-inflammatory activities of different microalgal extracts [ 16 ]. Dunaliella salina ( D. salina) microalgae are unicellular marine phytoplankton that belongs to the phylum Chlorophyta and family Dunaliellace [ 17 ] which contains large amounts of carotenoids at the stationary growth phase and has antioxidant and anticancer activity [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…Also, marine carotenoids have antioxidant properties by activation of the antioxidant network (GSH and catalase) [56] and scavenging reactive oxygen species (ROS) [57]. D. salina, in another study, regulates oxidative stress and protects the liver from fibrosis [16].…”

mentioning

confidence: 98%

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Amelioration of Hepatic Encephalopathy Using Dunaliella salina Microalgae in Rats: Modulation of Hyperammonemia/TLR4

Baz

Elgohary

Salama

2021

BioMed Research International

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Hepatic encephalopathy (HE) is a neuropsychiatric disease that is developed as a complication of both acute and chronic liver failure affecting psychomotor dysfunction, memory, and concentration. This study is aimed at evaluating the therapeutic effects of Dunaliella salina (D. salina) microalgae in thioacetamide- (TAA-) induced HE in rats. HE was induced by TAA (200 mg/kg; i.p.) for three successive days. Forty male Wister albino rats were divided into 4 groups; the first group was served as a normal, and the second group was injected with TAA and served as TAA control. The third and fourth groups were administered D. salina (100 and 200 mg/kg; p.o.), respectively, after TAA injection for 7 days. The behavioral and biochemical markers as well as histological aspects of HE were estimated. This study revealed that TAA caused behavioral changes, oxidative stress, neuroinflammation, nuclear pyknosis, and neurons degeneration. D. salina improved liver function and decreased oxidative stress and inflammatory mediator as TLR4 protein expression. Also, D. salina elevated HSP-25 and IGF-1 as well as improved brain histopathological alterations. In conclusion, D. salina exerted a therapeutic potential against HE via its antioxidant, antiinflammatory and cytoprotective effects.

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“…D . salina , in another study, regulates oxidative stress and protects the liver from fibrosis [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Amelioration of Hepatic Encephalopathy Using Dunaliella salina Microalgae in Rats: Modulation of Hyperammonemia/TLR4

Baz

Elgohary

Salama

2021

BioMed Research International

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“…The administration of H. pluvialis carotenoids astaxanthin enhanced the levels of SOD and CAT and decreased the lipid peroxidation against liver injury induced by doxorubicin in rats [ 42 ]. Also, β -carotene, from other algae D. Saline , exerted its antioxidant through inducing catalase and thioredoxin enzymes that attenuated STZ-induced diabetic neuropathy [ 43 ] and elevated the levels of GSH with a decrease in MDA opposing hepatic fibrosis induced by TAA in rats [ 44 ]. β -Cryptoxanthin, one of the other carotenoids, found in our crude extract has an antioxidant effect against oxidative DNA damage and lipid peroxidation [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Haematococcus pluvialis Carotenoids Enrich Fractions Ameliorate Liver Fibrosis Induced by Thioacetamide in Rats: Modulation of Metalloproteinase and Its Inhibitor

Baz

Salama

Ali

et al. 2021

BioMed Research International

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Hepatic fibrosis is a consequence of chronic liver diseases. Metalloproteinase and its inhibitor have crucial roles in the resolution of liver fibrosis. The current relevant study is aimed to evaluate the therapeutic effect of Haematococcus pluvialis (H. pluvialis) extract, astaxanthin-rich fraction, astaxanthin ester-rich fraction, and β-carotene-rich fraction as well as their mechanisms of action in curing hepatic fibrosis induced by thioacetamide (TAA). Liver fibrosis was induced using TAA (intraperitoneal injection, two times a week for 6 weeks), in a rat model and H. pluvialis extract (200 mg/kg), and other fractions (30 mg/kg) were orally administered daily for 4 weeks after the last TAA injection. Based on HPLC analysis, H. pluvialis extract contains β-carotene (12.95 mg/g, extract) and free astaxanthin (10.85 mg/g, extract), while HPLC/ESI-MS analysis revealed that H. pluvialis extract contains 28 carotenoid compounds including three isomers of free astaxanthin, α or β-carotene, lutein, 14 astaxanthin mono-esters, 5 astaxanthin di-esters, and other carotenoids. H. pluvialis and its fractions reduced liver enzymes, nitric oxide, collagen 1, alpha-smooth muscle actin, and transforming growth factor-beta as well as elevated catalase antioxidant activity compared to the TAA group. Also, H. pluvialis extract and its fractions exceedingly controlled the balance between metalloproteinase and its inhibitor, activated Kupffer cells proliferation, and suppressed liver apoptosis, necrobiosis, and fibrosis. These findings conclude that H. pluvialis extract and its fractions have an antifibrotic effect against TAA-induced liver fibrosis by regulating the oxidative stress and proinflammatory mediators, suppressing multiple profibrogenic factors, and modulating the metalloproteinase and its inhibitor pathway, recommending H. pluvialis extract and its fractions for the development of new effective medicine for treating hepatic fibrosis disorders.

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“…The same volumes of distilled water was given to the control group. The animals were checked for mortality for 24 hr (El Kady et al, 2015; El‐Baz, et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Metabolic profiling and in vivo hepatoprotective activity ofMalpighiaglabraL. leaves

et al. 2020

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Although Malpighia glabra Linn. fruits are well studied for their nutritional and medicinal prominence; little attention has been given to the leaves. Our study intends to investigate the leaves metabolic profile using Q-TOF LC/MS/MS (Quadrupole-Time-of-Flight-Liquid-Chromatography-Mass-Spectrometry), and to explore their in vivo hepatoprotective activity in rats using CCL 4-induced hepatic damage model and silymarin as standard. Fifty metabolites were characterized, belonging to different classes; coumarins (capensine, daphnoretin, and scopoletin), flavonoids (mainly quercetin and apigenin glycosides), phenolic acids (cinnamic acid and quinic acid derivatives) and amino acids (adenosine, homoisoleucine, and phenylalanine).These compounds are detected in the leaves for the first time. The hepatoprotective activity at three doses (200, 400, and 800 mg/kg) was investigated. The dose of 800 mg/Kg showed the highest hepatoprotective effect as it reduced the elevated serum levels of ALT, AST, NO, and TNF-α liver content by 26, 24, 23, and 42%, respectively, it also remarkably increased the serum level of catalase by 102%. All the tested doses showed higher reduction in serum level of TNF-α compared to silymarin which suggests their strong anti-inflammatory potential. M. glabra leaves are revealed to be a rich source of secondary metabolites and proved to possess significant hepatoprotective potential. 2 | MATERIAL AND ME THODS 2.1 | Plant material Fresh M. glabra L. leaves were gathered from Orman Botanic Garden since May 2018, it was kindly authenticated by Dr. Moustafa Mohammed Abd El-Kader, head of the herbarium at Orman Botanic Garden, Egypt. A voucher specimen of M. glabra L. was reserved at the Pharmacognosy Department, Faculty of Pharmacy, Cairo University with serial number 1.3.1.2019(4). 2.2 | Chemicals, reagents, and kits Chemicals and reagents: Ethanol for extraction was supplied from El-Gomhorya Company, Egypt. For LC/MSMS analysis; HPLC grade solvents were acquired from Fisher Scientific Chemicals. For biological activities; dimethyl sulfoxide (DMSO) was purchased from Loba Chemie for Laboratory Reagents and Fine Chemicals for industrial use, CCL 4 was acquired from El-Gomhorya Company for drugs and chemicals, Egypt. Standard silymarin was purchased from Sigma-Aldrich (USA). Kits: Nitric oxide (NO), Catalase, Alanine transaminase (ALT), Malondialdehyde (MDA), and Aspartate transaminase (AST), were all purchased from Biodiagnostic, Inc., (Egypt). Enzyme-linked immunosorbent assay (ELISA) kit was bought from Glory science co. for Tumor necrosis factor-α (TNF-α). 2.3 | Extract preparation M. glabra L. leaves (450 g) were air dried and powdered. They were macerated in 80% ethanol for 2 week at room temperature and filtered. Using a rotary evaporator, the ethanol was evaporated at 45°C. Maceration, filtration and evaporation processes were repeated till exhaustion to give 74.24 g dry residue. 2.4 | Q-TOF LC/MS/MS analysis Sample preparation and detailed analysis conditions are provided in supplementary file S-...

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Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats

Cited by 33 publications

References 49 publications

Amelioration of Hepatic Encephalopathy Using Dunaliella salina Microalgae in Rats: Modulation of Hyperammonemia/TLR4

Amelioration of Hepatic Encephalopathy Using Dunaliella salina Microalgae in Rats: Modulation of Hyperammonemia/TLR4

Haematococcus pluvialis Carotenoids Enrich Fractions Ameliorate Liver Fibrosis Induced by Thioacetamide in Rats: Modulation of Metalloproteinase and Its Inhibitor

Metabolic profiling and in vivo hepatoprotective activity ofMalpighiaglabraL. leaves

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