Dünnschicht‐Chromatographie auf dem Fettgebiet X: Die Analyse von Stoffwechsel‐Lipoiden

Abstract

In der vorliegenden Arbeit wird die Anwendung der Dünnschicht‐Chromatographie auf die qualitative und quantitative Analyse biologisch wichtiger Lipoide beschrieben. Es wurden analysiert: die Gesamtlipoide von Tierblut und Tierlebern sowie des Urins eines an Lipuria Chyluria erkrankten Menschen.

Chromatography

“…TLC (1455) and spread-layer chromatography (1386) have by now been successfully applied to all classes of steroids. Thin-layer partition chromatography uses a mixture of some inert carrier and gypsum to support a stationary phase of formamide (1624), undecane (1202), or paraffin oil (1044)• All three techniques are applicable to sterols (54,115,233,794,815,1083,1131,1623,1763), vitamins D (354,1141), steroidal sapogenins (114, 969,1373) and alkaloids (7,173,1373), bile acids (431,691,693,875,1387), cardenolides (688,954,1441,1511) and bufadienolides (730,1509,1756), and steroids related to the estrogenic (377,472,667,763,936,953,1025,1533), androgenic (253,399,407,557,867,1024,1292), progestational (53,…”

“…For adsorption chromatography of simple lipids, silicic acid-impregnated paper (139), unbound layers of alumina (1623), or conventional thin layers of silica gel (42,794), silver nitrate-impregnated silica gel (87), and hydroxyapatite (694) may be used.…”