Duplex realtime PCR method for Epstein–Barr virus and human DNA quantification: its application for post-transplant lymphoproliferative disorders detection

Fellner, Marı́a Dolores; Durand, Karina; Rodríguez, Marisa; Irazú, Lucía; Alonio, Lidia Virginia; Picconi, Marı́a Alejandra

doi:10.1016/j.bjid.2013.07.011

Cited by 15 publications

(13 citation statements)

References 42 publications

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“…Therefore, low-cost, POC molecular methods of EBV quantification could transform care in settings where the virus is endemic and rapid diagnosis is essential to improving BL outcomes. Particularly in areas where limited access to histopathology services can delay a definitive diagnosis of rapidly advancing BL, recent advances in POC methods to detect clonal IGH gene rearrangement and EBV viral load offer critical shorter turnaround times [75] , [76] . The tests can be applied to blood, aspirate, or biopsy samples to support a clinical diagnosis of cancer while awaiting confirmation with cytology or histology results [75] – [77] .…”

Section: \Documentclass[12pt]{minimal} \Usepackage{amsmath} \Usepackamentioning

confidence: 99%

“…Particularly in areas where limited access to histopathology services can delay a definitive diagnosis of rapidly advancing BL, recent advances in POC methods to detect clonal IGH gene rearrangement and EBV viral load offer critical shorter turnaround times [75] , [76] . The tests can be applied to blood, aspirate, or biopsy samples to support a clinical diagnosis of cancer while awaiting confirmation with cytology or histology results [75] – [77] . With further development and validation, these tests have the potential to greatly improve the diagnosis and monitoring of EBV-associated cancers in resource-limited settings.…”

Section: \Documentclass[12pt]{minimal} \Usepackage{amsmath} \Usepackamentioning

confidence: 99%

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The Role of Affordable, Point-of-Care Technologies for Cancer Care in Low- and Middle-Income Countries: A Review and Commentary

Haney

Tandon

Divi

et al. 2017

IEEE J. Transl. Eng. Health Med.

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As the burden of non-communicable diseases such as cancer continues to rise in low- and middle-income countries (LMICs), it is essential to identify and invest in promising solutions for cancer control and treatment. Point-of-care technologies (POCTs) have played critical roles in curbing infectious disease epidemics in both high- and low-income settings, and their successes can serve as a model for transforming cancer care in LMICs, where access to traditional clinical resources is often limited. The versatility, cost-effectiveness, and simplicity of POCTs warrant attention for their potential to revolutionize cancer detection, diagnosis, and treatment. This paper reviews the landscape of affordable POCTs for cancer care in LMICs with a focus on imaging tools, in vitro diagnostics, and treatment technologies and aspires to encourage innovation and further investment in this space.

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Section: \Documentclass[12pt]{minimal} \Usepackage{amsmath} \Usepackamentioning

confidence: 99%

Section: \Documentclass[12pt]{minimal} \Usepackage{amsmath} \Usepackamentioning

confidence: 99%

The Role of Affordable, Point-of-Care Technologies for Cancer Care in Low- and Middle-Income Countries: A Review and Commentary

Haney

Tandon

Divi

et al. 2017

IEEE J. Transl. Eng. Health Med.

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“…Analyses of the in situ hybridization to assess the presence of EBV in HIV-infected patients in the saliva, was determined by PCR, whereas the amount of DNA in EBV-positive samples was estimated by titration repeated. The results showed that all patients with hairy leukoplakia EBV-DNA contained saliva [22][23][24][25].…”

Section: Saliva Testsmentioning

confidence: 94%

“…HIV-infected individuals and healthy controls showed a similar frequency of detection of viral DNA. EBV DNA was significantly amplified in the saliva of family members of HIV/EBV co-infected individuals, from here the possibilities for comparative analysis of salivary diagnostics [25,29,[35][36][37].…”

Section: Saliva Testsmentioning

confidence: 99%

Physiological Changes in Salivary Gland and Kidney that help the Diagnosis caused of Epstein-Barr virus: A Brief Review

Lp¹,

Kct²,

Aba³

et al. 2016

J Microb Biochem Technol

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“…Currently, while several protocols exist for identification of the herpesvirus genome (11), multiple PCR assays are required for the process. In recent years, real-time PCR has been increasingly applied to simplify the overall procedure and reduce performance time in detecting and quantifying virus in clinical samples (12,13). Multiplex qPCR has been suggested as an efficient alternative for simultaneous detection of HCMV, HHV-6 and HHV-7 within the same reaction (9,14).…”

Section: Introductionmentioning

confidence: 99%

Multiplex Qpcr Facilitates Identification Of Betaherpesviruses In Patients With Acute Liver Failure Of Unknown Etiology

Raposo

Alves

Silva

et al. 2019

Preprint

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Background: The etiology of acute liver failure (ALF) is often unknown and reported to be associated with herpesviruses in a number of cases. In this study, we examined for betaherpesviruses infections in patients with ALF of unknown etiology using a multiplex qPCR to Betaherpesviruses subfamily. Methods: Liver and serum samples from 27 patients with ALF of unknown etiology were analyzed with the aid of multiplex qPCR to identify betaherpesviruses. All positive samples were sequenced to confirm herpes infection and liver enzyme levels evaluated. Results: Betaherpesviruses infection was effectively detected using multiplex qPCR. Seven (26%), two (7%) and three (11%) cases were positive for HHV-6, HHV-7 and HCMV, respectively. Two cases of dual infection (HHV-7/HHV-6 and HHV-7/HCMV) were additionally identified. Interestingly, HHV-7 was only detected in the presence of other betaherpesviruses. Sequencing information confirmed betaherpesviruses infection. High hepatic enzyme levels and INR values>1.4 were determined in all betaherpesvirus-positive patients. Conclusions: Multiplex qPCR facilitated efficient quantification, indicating that differentiation between betaherpesviruses is possible with the sole use of real-time PCR. Liver and serum samples were positive for some betaherpesviruses, suggesting an association with ALF. Coinfection of HHV-7 with HHV-6 or HCMV was additionally detected, suggesting that the precursor betaherpesviruses infection can trigger HHV-7 infection. Based on these results, we propose that ALF patients should be screened for the presence of betaherpesviruses.

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Duplex realtime PCR method for Epstein–Barr virus and human DNA quantification: its application for post-transplant lymphoproliferative disorders detection

Abstract: The performance of the assay was suitable for the required clinical application. The assay may be useful to monitor EBV infection in transplant patients, in particular in laboratories from low-income regions that cannot afford to use commercial assays.

Cited by 15 publications

References 42 publications

The Role of Affordable, Point-of-Care Technologies for Cancer Care in Low- and Middle-Income Countries: A Review and Commentary

The Role of Affordable, Point-of-Care Technologies for Cancer Care in Low- and Middle-Income Countries: A Review and Commentary

Physiological Changes in Salivary Gland and Kidney that help the Diagnosis caused of Epstein-Barr virus: A Brief Review

Multiplex Qpcr Facilitates Identification Of Betaherpesviruses In Patients With Acute Liver Failure Of Unknown Etiology

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