Duration of the foot-and-mouth disease virus antibody response in mice is closely related to the presence of antigen-specific presenting cells.

Wigdorovitz, Andrés; Zamorano, Patricia; Fm, Fernández; Lopez, Osvaldo J.; Prato-Murphy, M; Carrillo, C.; Am, Sadir; Mv, Borca

doi:10.1099/0022-1317-78-5-1025

Cited by 12 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This antibody response was also correlated with a specific FMD cellular immune response involving CD4 T cells. Other mechanisms like the persistence of antigen presenting cells may be involved in the maintenance of an FMD antibody response, as demonstrated in mice [15].…”

Section: Discussionmentioning

confidence: 97%

Residual foot-and-mouth disease virus antibodies in French cattle and sheep six years after the vaccination ban

Remond¹,

Kaiser²,

Lebreton³

et al. 2001

Vet. Res.

View full text Add to dashboard Cite

-A serological survey was carried out on French cattle to establish a reference pattern of residual vaccine antibodies and non-specific reactions against the foot-and-mouth disease virus 6 years after the ban on vaccination and in the absence of any foot-and-mouth disease outbreak. Most of the multi-vaccinated cattle still displayed high titres of antibodies and up to 50% of those which had received a single injection still had antibodies. Non-specific reactors were also recorded among animals born during and after 1991. Most of them displayed low titres close to the threshold. Sheep were also tested and, as for cattle, 4.6% of non-specific reactors were recorded, with titres close to the threshold for two-thirds of them. As part of these animals have been resampled and retested, sera revealed negative confirming that these animals are true non-specific reactors. Serological testing as a mean of FMD control should take these facts into account.foot-and-mouth disease / serology / vaccination / cattle / sheep Résumé -Persistance des anticorps contre le virus de la fièvre aphteuse dans le cheptel bovin et ovin français six années après l'arrêt de la vaccination. Une enquête sérologique a été entreprise afin de déterminer hors de tout contexte d'épidémie de fièvre aphteuse, le profil de réponse sérolo-gique du cheptel vis-à-vis du virus de la fièvre aphteuse six années après l'arrêt de la vaccination. Cette enquête a permis également de déterminer l'importance des réactions non spécifiques chez les bovins nés après l'arrêt de la vaccination ainsi que chez les ovins. La majorité des bovins nés avant 1989 étaient réagissants et présentaient encore de forts taux d'anticorps. Parmi les animaux n'ayant reçu qu'une seule injection de vaccin, 50 % étaient encore positifs. Des réactions non spécifiques ont également été observées chez les animaux nés en et après 1991, la majorité de ces réactions se situant près du seuil de positivité. Parmi les ovins testés, 4,6 % de réactions non spécifiques ont été relevées dont les 2/3 étaient aussi proches du seuil de positivité. Des prélèvements ont pu être refaits sur une partie de Vet. Res. 32 (2001) [81][82][83][84][85][86] 81

show abstract

Section: Discussionmentioning

confidence: 97%

Residual foot-and-mouth disease virus antibodies in French cattle and sheep six years after the vaccination ban

Remond¹,

Kaiser²,

Lebreton³

et al. 2001

Vet. Res.

View full text Add to dashboard Cite

show abstract

“…The humoral immune response against infectious FMDV proceeds in two phases: an early thymus-independent (TI) phase, involved in viral clearance, and a late thymus-dependent (TD) memory phase (8,60). A similar type of response has been described for other viruses such as vesicular stomatitis virus (VSV) (5, 58).…”

Section: Infection Of Mice With Cytopathic Foot-and-mouth Disease Virmentioning

confidence: 88%

The Early Protective Thymus-Independent Antibody Response to Foot-and-Mouth Disease Virus Is Mediated by Splenic CD9⁺B Lymphocytes

Ostrowski

Vermeulen

Zábal

et al. 2007

J Virol

View full text Add to dashboard Cite

Infection of mice with cytopathic foot-and-mouth disease virus (FMDV) induces a rapid and specific thymus-independent (TI) neutralizing antibody response that promptly clears the virus. Herein, it is shown that FMDV-infected dendritic cells (DCs) directly stimulate splenic innate-like CD9؉ B lymphocytes to rapidly (3 days) produce neutralizing anti-FMDV immunoglobulin M antibodies without T-lymphocyte collaboration. In contrast, neither follicular (CD9 ؊ ) B lymphocytes from the spleen nor B lymphocytes from lymph nodes efficiently respond to stimulation with FMDV-infected DCs. The production of these protective neutralizing antibodies is dependent on DC-derived interleukin-6 (IL-6) and on CD9؉ cell-derived IL-10 secretion. In comparison, DCs loaded with UV-inactivated FMDV are significantly less efficient in directly stimulating B lymphocytes to secrete TI antibodies. A critical role of the spleen in the early production of anti-FMDV antibodies in infected mice was also demonstrated in vivo. Indeed, either splenectomy or functional disruption of the marginal zone of the spleen delays and reduces the magnitude of the TI anti-FMDV antibody response in infected mice. Together, these results indicate that in addition to virus localization, the FMDV-mediated modulation of DC functionality is a key parameter that collaborates in the induction of a rapid and protective TI antibody response against this virus.Experimental infection of mice with serotype O of foot-andmouth disease virus (FMDV), the prototypic member of the genus Aphthovirus of the family Picornaviridae, causes a subclinical infection characterized by viral replication in the pancreas and a viremia that lasts for 48 to 72 h (14, 17). At this time, concurrently with the appearance of serum neutralizing antibodies, the virus is cleared from circulation (8, 17).The humoral immune response against infectious FMDV proceeds in two phases: an early thymus-independent (TI) phase, involved in viral clearance, and a late thymus-dependent (TD) memory phase (8,60). A similar type of response has been described for other viruses such as vesicular stomatitis virus (VSV) (5, 58). We have recently shown that during the early phase of the response against infectious FMDV, there is a transient but generalized suppression of TD responses (51). This effect is mediated, at least in part, by the downregulation of major histocompatibility complex class II and CD40 molecules on dendritic cells (DCs) and by the production of interleukin-10 (IL-10) by splenocytes (51). In contrast, the antibody response against UV-inactivated FMDV (UV-FMDV) proceeds as a typical TD response. Thus, it exhibits a delayed induction (ϳ1 week). Moreover, the antibody isotypes elicited by the inactivated virus are different from those induced by infectious FMDV (52).DCs are potent stimulators of T-cell responses (56). In addition, DCs also have the ability to influence B-cell functionality by inducing isotype switching, differentiation towards the plasma cell, and antibody secretion (16). The stimulati...

show abstract

“…Although in athymic mice, Ab titers decline from the tenth day after infection, reaching nonprotective levels by day 21, euthymic mice infected with FMDV maintain high levels of neutralizing Abs for more than a year (3). Furthermore, the maintenance of the FMDV-Ab response in infected and vaccinated mice is dependent on the presence of MHC class IIrestricted APCs (6). Thus, the Ab response against infectious FMDV is initiated as a TI response, but T cell collaboration is required for long-term maintenance of Ab titers in serum.…”

Section: F Oot-and-mouth Disease (Fmd)mentioning

confidence: 99%

Impairment of Thymus-Dependent Responses by Murine Dendritic Cells Infected with Foot-and-Mouth Disease Virus

Ostrowski

Vermeulen

Zábal

et al. 2005

The Journal of Immunology

View full text Add to dashboard Cite

Foot-and-mouth disease virus (FMDV) is a cytopathic virus that experimentally infects mice, inducing a thymus-independent neutralizing Ab response that rapidly clears the virus. In contrast, vaccination with UV-inactivated virus induces a typical thymus-dependent (TD) response. In this study we show that dendritic cells (DCs) are susceptible to infection with FMDV in vitro, although viral replication is abortive. Infected DCs down-regulate the expression of MHC class II and CD40 molecules and up-regulate the expression of CD11b. In addition, infected DCs exhibit morphological and functional changes toward a macrophage-like phenotype. FMDV-infected DCs fail to stimulate T cell proliferation in vitro and to boost an Ab response in vivo. Moreover, infection of DCs in vitro induces the secretion of IFN-γ and the suppressive cytokine IL-10 in cocultures of DCs and splenocytes. High quantities of these cytokines are also detected in the spleens of FMDV-infected mice, but not in the spleens of vaccinated mice. The peak secretion of IFN-γ and IL-10 is concurrent with the suppression of Con A-mediated proliferation of T cells obtained from the spleens of infected mice. Furthermore, the secretion of these cytokines correlates with the suppression of the response to OVA, a typical TD Ag. Thus, infection of DCs with FMDV induces suppression of TD responses without affecting the induction of a protective thymus-independent response. Later, T cell responses are restored, setting the stage for the development of a long-lasting protective immunity.

show abstract

Duration of the foot-and-mouth disease virus antibody response in mice is closely related to the presence of antigen-specific presenting cells.

Cited by 12 publications

References 12 publications

Residual foot-and-mouth disease virus antibodies in French cattle and sheep six years after the vaccination ban

Residual foot-and-mouth disease virus antibodies in French cattle and sheep six years after the vaccination ban

The Early Protective Thymus-Independent Antibody Response to Foot-and-Mouth Disease Virus Is Mediated by Splenic CD9⁺B Lymphocytes

Impairment of Thymus-Dependent Responses by Murine Dendritic Cells Infected with Foot-and-Mouth Disease Virus

Contact Info

Product

Resources

About

Duration of the foot-and-mouth disease virus antibody response in mice is closely related to the presence of antigen-specific presenting cells.

Cited by 12 publications

References 12 publications

Residual foot-and-mouth disease virus antibodies in French cattle and sheep six years after the vaccination ban

Residual foot-and-mouth disease virus antibodies in French cattle and sheep six years after the vaccination ban

The Early Protective Thymus-Independent Antibody Response to Foot-and-Mouth Disease Virus Is Mediated by Splenic CD9+B Lymphocytes

Impairment of Thymus-Dependent Responses by Murine Dendritic Cells Infected with Foot-and-Mouth Disease Virus

Contact Info

Product

Resources

About

The Early Protective Thymus-Independent Antibody Response to Foot-and-Mouth Disease Virus Is Mediated by Splenic CD9⁺B Lymphocytes