Dusky works upstream of Four‐jointed and Forked in wing morphogenesis in <i>Tribolium castaneum</i>

Li, C; Li, B; Ma, Shangshang; Lu, Ping; Chen, K

doi:10.1111/imb.12327

Cited by 7 publications

(24 citation statements)

References 50 publications

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“…However, the mechanism by which chitin-degrading genes control the development of wings remains unclear. Previous studies demonstrated that these genes, such as dusky (DY), wingless (WG), vestigial (VG), and apterous (AP), have essential roles during the wing morphogenesis of T. castaneum [62,63]. Here, we found that the expression levels of LsDY, LsWG, LsVG, and LsAP were downregulated after the knockdown of LsCDA1 in L. serricorne.…”

Section: The Function Of Lscda1 Involved In Wing Development During Pmentioning

confidence: 67%

Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne

Yang

Yan

et al. 2020

IJMS

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Chitin deacetylases (CDAs) are chitin-modifying enzymes known to play vital roles in insect metamorphosis and development. In this study, we identified and characterized a chitin deacetylase 1 gene (LsCDA1) from the cigarette beetle Lasioderma serricorne. LsCDA1 contains a 1614 bp open reading frame encoding a protein of 537 amino acids that includes domain structures typical of CDAs. LsCDA1 was mainly expressed in the late larval and late pupal stages. In larval tissues, the highest level of LsCDA1 was detected in the integument. The expression of LsCDA1 was induced by 20-hydroxyecdysone (20E) in vivo, and it was significantly suppressed by knocking down the expression of ecdysteroidogenesis genes and 20E signaling genes. RNA interference (RNAi)-aided silencing of LsCDA1 in fifth-instar larvae prevented the larval–pupal molt and caused 75% larval mortality. In the late pupal stage, depletion of LsCDA1 resulted in the inhibition of pupal growth and wing abnormalities, and the expression levels of four wing development-related genes (LsDY, LsWG, LsVG, and LsAP) were dramatically decreased. Meanwhile, the chitin contents of LsCDA1 RNAi beetles were significantly reduced, and expressions of three chitin synthesis pathway genes (LsTRE1, LsUAP1, and LsCHS1) were greatly decreased. The results suggest that LsCDA1 is indispensable for larval–pupal and pupal–adult molts, and that it is a potential target for the RNAi-based control of L. serricorne.

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Section: The Function Of Lscda1 Involved In Wing Development During Pmentioning

confidence: 67%

Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne

Yang

Yan

et al. 2020

IJMS

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“…Furthermore, 95.93 ± 0.15% of ds‐ forked adults died 7 days after eclosion (Figure d). Singed and miniature silencing showed different effects from those of crinkled RNAi (Li, Li, Ma, Lu, & Chen, ).…”

Section: Resultsmentioning

confidence: 99%

“…One possible reason for this is that RNAi on svb deleted more mRNA levels of itself than that by crinkled knockdown (Figure b; Li et al., ). The transcription of svb was regulated by a number of genes in the insect (Chanut‐Delalande et al., ; Li et al., ; Payre et al., ), and the roles of svb in pupation, eclosion, and epidermal pigmentation are mainly controlled by other genes not crinkled . Downstream genes of svb likely mediate the function of crinkled in wing morphogenesis in the insect.…”

Section: Discussionmentioning

confidence: 99%

“…These results demonstrate the involvement of these genes in the crinkled pathway. Of these genes, a mutation in miniature reduces wing size in D. melanogaster (Roch, Alonso, & Akam, ), and a recent study showed that miniature knockdown impairs pupation in T. castaneum (Li et al., ). Singed is required for actin bundle formation during oogenesis and bristle extension in D. melanogaster (Cant, Knowles, Mooseker, & Cooley, ), but RNAi‐mediated reduction of its expression had no effects in T. castaneum (Li et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Of these genes, a mutation in miniature reduces wing size in D. melanogaster (Roch, Alonso, & Akam, ), and a recent study showed that miniature knockdown impairs pupation in T. castaneum (Li et al., ). Singed is required for actin bundle formation during oogenesis and bristle extension in D. melanogaster (Cant, Knowles, Mooseker, & Cooley, ), but RNAi‐mediated reduction of its expression had no effects in T. castaneum (Li et al., ). Dyl functions in the formation of embryonic denticles (Fernandes et al., ), cuticle deposition during bristle development (Nagaraj & Adler, ) and the integrity and planar cell polarity of wing hairs of D. melanogaster (Adler, Sobala, Thom, & Nagaraj, ).…”

Section: Discussionmentioning

confidence: 99%

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Crinkled employs wingless pathway for wing development in Tribolium castaneum

Liu

et al. 2018

Arch Insect Biochem Physiol

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Crinkled is associated with embryonic denticle formation and auditory organ development in Drosophila melanogaster. However, the functions of Crinkled have not been fully investigated. Additionally, the genes that participate in the Crinkled pathway are unknown. Phylogenetic analysis indicates that crinkled exhibits a one-to-one orthologous relationship in insects. In Tribolium castaneum, the crinkled gene is 6,498 bp in length and consists of six exons. Crinkled expression peaked during two phases in Tribolium: late embryonic and pupal stages. High levels of crinkled mRNA were detected in the fat body, head, epidermis, ovary, and accessory gland of late adults. Knockdown of crinkled using RNA interference (RNAi) severely affected wing morphogenesis in T. castaneum. We further showed that crinkled silencing reduced forked expression through wingless and shaven-baby, and RNAi of forked phenocopied the effects of crinkled knockdown in T. castaneum. This study investigated the development role of crinkled in postembryonic stages and indicated that forked mediates the functions of crinkled during wing morphogenesis in T. castaneum.

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The zona pellucida protein piopio regulates the metamorphosis and reproduction in Tribolium castaneum

Zhang,

Ge,

Yang

et al. 2024

Arch Insect Biochem Physiol

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The zona pellucida domain protein piopio (Pio) was only reported to mediate the adhesion of the apical epithelial surface and the overlying apical extracellular matrix in Drosophila melanogaster, but the developmental roles of Pio were poorly understood in insects. To address this issue, we comprehensively analyzed the function of Pio in Tribolium castaneum. Phylogenetic analysis indicated that pio exhibited one‐to‐one orthologous relationship among insects. T. castaneum pio had a 1236‐bp ORF and contained eight exons. During development pio was abundantly expressed from larva to adult and lowly expressed at the late stage of embryo and adult, while it had more transcripts in the head, epidermis, and gut but fewer in the fat body of late‐stage larvae. Knockdown of pio inhibited the pupation, eclosion, and reproduction of T. castaneum. The expression of vitellogenin 1 (Vg1), Vg2, and Vg receptor (VgR) largely decreased in pio‐silenced female adults. Silencing pio increased the 20‐hydroxyecdysone titer by upregulating phm and spo expression but decreased the juvenile hormone (JH) titer through downregulating JHAMT3 and promoting JHE, JHEH‐r4, and JHDK transcription. These results suggested that Pio might regulate the metamorphosis and reproduction via modulating the ecdysone and JH metabolism in T. castaneum. This study found the novel roles of pio in insect metamorphosis and reproduction, and provided the new insights for analyzing other zona pellucida proteins functions in insects.

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Dusky works upstream of Four‐jointed and Forked in wing morphogenesis in Tribolium castaneum

Cited by 7 publications

References 50 publications

Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne

Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne

Crinkled employs wingless pathway for wing development in Tribolium castaneum

The zona pellucida protein piopio regulates the metamorphosis and reproduction in Tribolium castaneum

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