DWT1/DWL2 act together with OsPIP5K1 to regulate plant uniform growth in rice

Fang, Fang; Ye, Shiwei; Tang, Jingyao; Bennett, Malcolm; Liang, Wanqi

doi:10.1111/nph.16216

Cited by 22 publications

(14 citation statements)

References 72 publications

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“…5 a, b), suggesting that LOC_Os03g03480 might be a candidate gene involved in panicle length. Besides these 6 genes, OsSWN5 and LOC_Os03g03260 in qPL3-3 were of interest as other genes annotated as “no apical meristem protein” and “homeobox domain containing protein” ( LOC_Os01g47710 and LOC_Os05g48990 ) had previously been shown to have a role in controlling panicle length in rice (Wang et al 2014 ; Sakuraba et al 2015 ; Fang et al 2020 ), so OsSWN5 and LOC_Os03g03260 in qPL3-3 were of interest. Similarly, panicle length is sensitive to genes involved in plant hormone biosynthesis or signaling, including ABA (Wang et al 2015b ; Hong et al 2016 ; Zhang et al 2016 ), so DSM2 ( LOC_Os03g03370 ), which encodes a β-carotene hydroxylase involved in the biosynthesis of the ABA precursor zeaxanthin (Du et al 2010 ), was also a gene of interest in qPL3-3.…”

Section: Resultsmentioning

confidence: 99%

Dissection of the Genetic Basis of Rice Panicle Architecture Using a Genome-wide Association Study

Bai

Hong

et al. 2021

Rice

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Panicle architecture is one of the major factors influencing productivity of rice crops. The regulatory mechanisms underlying this complex trait are still unclear and genetic resources for rice breeders to improve panicle architecture are limited. Here, we have performed a genome-wide association study (GWAS) to analyze and identify genetic determinants underlying three panicle architecture traits. A population of 340 rice accessions from the 3000 Rice Genomes Project was phenotyped for panicle length, primary panicle number and secondary branch number over two years; GWAS was performed across the whole panel, and also across the japonica and indica sub-panels. A total of 153 quantitative trait loci (QTLs) were detected, of which 5 were associated with multiple traits, 8 were unique to either indica or japonica sub-panels, while 37 QTLs were stable across both years. Using haplotype and expression analysis, we reveal that genetic variations in the OsSPL18 promoter significantly affect gene expression and correlate with panicle length phenotypes. Three new candidate genes with putative roles in determining panicle length were also identified. Haplotype analysis of OsGRRP and LOC_Os03g03480 revealed high association with panicle length variation. Gene expression of DSM2, involved in abscisic acid biosynthesis, was up-regulated in long panicle accessions. Our results provide valuable information and resources for further unravelling the genetic basis determining rice panicle architecture. Identified candidate genes and molecular markers can be used in marker-assisted selection to improve rice panicle architecture through molecular breeding.

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Section: Resultsmentioning

confidence: 99%

Dissection of the Genetic Basis of Rice Panicle Architecture Using a Genome-wide Association Study

Bai

Hong

et al. 2021

Rice

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“…Depending on the target genes, these TFs might act as positive or negative regulators of their expression. The rice ANK and ANK-TPR genes described here display co-expression during panicle development with several TF genes from the AP2/ERF, WUSCHEL-related homeobox (WOX) and Auxin Response Factor (ARF) families known to be involved in the regulation of inflorescence structure [ 28 , 42 , 44 – 48 ]. The latter groups therefore provide good candidates for further investigations of the regulatory network in which the ANK and ANK-TPR genes operate.…”

Section: Discussionmentioning

confidence: 99%

A cluster of Ankyrin and Ankyrin-TPR repeat genes is associated with panicle branching diversity in rice

Khong

Thị

et al. 2021

PLoS Genet

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The number of grains per panicle is an important yield-related trait in cereals which depends in part on panicle branching complexity. One component of this complexity is the number of secondary branches per panicle. Previously, a GWAS site associated with secondary branch and spikelet numbers per panicle in rice was identified. Here we combined gene capture, bi-parental genetic population analysis, expression profiling and transgenic approaches in order to investigate the functional significance of a cluster of 6 ANK and ANK-TPR genes within the QTL. Four of the ANK and ANK-TPR genes present a differential expression associated with panicle secondary branch number in contrasted accessions. These differential expression patterns correlate in the different alleles of these genes with specific deletions of potential cis-regulatory sequences in their promoters. Two of these genes were confirmed through functional analysis as playing a role in the control of panicle architecture. Our findings indicate that secondary branching diversity in the rice panicle is governed in part by differentially expressed genes within this cluster encoding ANK and ANK-TPR domain proteins that may act as positive or negative regulators of panicle meristem’s identity transition from indeterminate to determinate state.

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“…However, both tomato and petunia have a cymose inflorescence pattern (determinate growth) and it is unclear whether these inflorescence‐associated defects are specific to cymose or are also exhibited by racemose (indeterminate growth) and panicle (mixed inflorescence) inflorescences. At least in Arabidopsis (racemose inflorescence), the role of WOX9 appears not to be restricted to inflorescence development, and in rice (mixed inflorescence), WOX9 is involved in uniform tiller growth and development (Wang et al ., 2014; Fang et al ., 2020).…”

Section: Discussionmentioning

confidence: 99%

“…New Phytologist exhibited by racemose (indeterminate growth) and panicle (mixed inflorescence) inflorescences. At least in Arabidopsis (racemose inflorescence), the role of WOX9 appears not to be restricted to inflorescence development, and in rice (mixed inflorescence), WOX9 is involved in uniform tiller growth and development (Wang et al, 2014;Fang et al, 2020).…”

Section: Researchmentioning

confidence: 99%

WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris

et al. 2020

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Summary WOX family transcription factors regulate multiple developmental programs. The intermediate clade transcriptional activator WOX9 functions together with the modern clade transcriptional repressor WOX genes in embryogenesis and meristems maintenance, but the mechanism of this interaction is unclear. STF and LAM1 are WOX1 orthologs required for leaf blade outgrowth in Medicago truncatula and Nicotiana sylvestris, respectively. Using biochemical methods and genome editing technology, here we show that WOX9 is an abaxial factor and functions antagonistically to STF and LAM1 to regulate leaf blade development. While NsWOX9 ectopic expression enhances the lam1 mutant phenotype, and antisense expression partially rescues the lam1 mutant, both overexpression and knockout of NsWOX9 in N. sylvestris resulted in a range of severe leaf blade distortions, indicating important role in blade development. Our results indicate that direct repression of WOX9 by WUS clade repressor STF/LAM1 is required for correct blade architecture and patterning in M. truncatula and N. sylvestris. These findings suggest that controlling transcriptional activation and repression mechanisms by direct interaction of activator and repressor WOX genes may be required for cell proliferation and differentiation homeostasis, and could be an evolutionarily conserved mechanism for the development of complex and diverse morphology in flowering plants.

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DWT1/DWL2 act together with OsPIP5K1 to regulate plant uniform growth in rice

Cited by 22 publications

References 72 publications

Dissection of the Genetic Basis of Rice Panicle Architecture Using a Genome-wide Association Study

Dissection of the Genetic Basis of Rice Panicle Architecture Using a Genome-wide Association Study

A cluster of Ankyrin and Ankyrin-TPR repeat genes is associated with panicle branching diversity in rice

WOX9 functions antagonistic to STF and LAM1 to regulate leaf blade expansion in Medicago truncatula and Nicotiana sylvestris

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