Dye affinity cryogels for plasmid DNA purification

Çimen, Duygu; Yılmaz, Fatma; Perçin, Işık; Türkmen, Deniz; Deni̇zli̇, Adil

doi:10.1016/j.msec.2015.06.041

Cited by 30 publications

(17 citation statements)

References 63 publications

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“…The heat and mass transfer of cryogels is faster than normal gels with the same chemical composition. Moreover, the response time of cryogels is fast at short distances in the thin walls of macroporous structures [ 30 , 31 , 32 , 33 ]. The flow channel in a cryogel is large enough due to the interconnected macropore structure.…”

Section: Cryogelsmentioning

confidence: 99%

Injectable Cryogels in Biomedicine

Çimen

Özbek

Bereli

et al. 2021

Gels

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Cryogels are interconnected macroporous materials that are synthesized from a monomer solution at sub-zero temperatures. Cryogels, which are used in various applications in many research areas, are frequently used in biomedicine applications due to their excellent properties, such as biocompatibility, physical resistance and sensitivity. Cryogels can also be prepared in powder, column, bead, sphere, membrane, monolithic, and injectable forms. In this review, various examples of recent developments in biomedical applications of injectable cryogels, which are currently scarce in the literature, made from synthetic and natural polymers are discussed. In the present review, several biomedical applications of injectable cryogels, such as tissue engineering, drug delivery, therapeutic, therapy, cell transplantation, and immunotherapy, are emphasized. Moreover, it aims to provide a different perspective on the studies to be conducted on injectable cryogels, which are newly emerging trend.

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Section: Cryogelsmentioning

confidence: 99%

Injectable Cryogels in Biomedicine

Çimen

Özbek

Bereli

et al. 2021

Gels

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“…It is also used in DNA purification, RNA adsorption and drug and enzyme transport [91,95,96]. These approaches shed light on the creation of new adjuvant systems for the transport of genetic material using pHEMA [91,93,97].…”

Section: Poly(2-hydroxyethyl Methacrylate) (Phema)mentioning

confidence: 99%

Current State of the Art in DNA Vaccine Delivery and Molecular Adjuvants: Bcl-xL Anti-Apoptotic Protein as a Molecular Adjuvant

Gülce-İz¹,

Sağlam-Metiner²

2019

Immune Response Activation and Immunomodulation

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DNA vaccines (nucleic acid vaccines) have been gaining importance as promising therapeutics against infectious diseases, cancer, autoimmune disorders and allergy for the past two decades. However, the immune responses elicited by the DNA vaccines are not at the desired level to stimulate a protective immune response. Thus, studies are focused on the enhancement of DNA vaccine-induced immune response by different approaches. The most common approach is to use biomaterial-based adjuvants for enhanced antigen delivery and uptake by antigenpresenting cells. Some of these adjuvants are alum, saponins, microspheres, nanoparticles, liposomes, polymers, etc. used in vaccine formulations. In addition, molecular adjuvants like cytokines, chemokines and heat shock proteins have been shown to be promising in designing DNA vaccines. In this chapter, molecular adjuvants to improve DNA vaccination-induced immune responses will be summarized with a special focus on Bcl-xL anti-apoptotic protein.

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“…Nowadays; numerous new affinity support materials including cryogels has been synthesized and developed for separation/purification studies [29]. Cryogels are prepared under semi-frozen conditions and possess several unique properties compared to traditional gels ( Figure 3).…”

Section: Dna Purification By Affinity Chromatographymentioning

confidence: 99%

DNA Purification by Solid Phase Extraction (SPE) Methods

Topçu¹,

Aşır²,

Türkmen³

2016

HJBC

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Ö Z E T D NA'nın saflaştırılması veya izole edilmesi moleküler biyoloji, tıbbi çalışmalar ve gen tedavi çalışmaları için önem taşır. Özellikle PCR çalışmaları için yüksek saflıkta DNA molekülüne ihtiyaç duyulmaktadır. Nükleik asitlerin saflaştırılmasında sırasıyla çözücü özütlemesi ve katı faz özütlemesi olmak üzere iki yöntem kulanılır. Çözücü ekstraksiyonu kolay uygulanabilir olmasına rağmen; toksisite, yüksek oranda çözücü ihtiyacı, yöntemin uzun zaman alması ve düşük özgüllüğü gibi birtakım kısıtlamalar mecvuttur. Günümüzde çeşitli örneklerden DNA'nın saflaştırılması için katı faz özütlemesi (SPE) yaygın olarak kullanılmaktadır. Bu çalışmada; özellikle DNA'nın saflaştırılması için SPE yönteminin uygulamaları üzerinde durulmuş ve bilimsel çalışmalar özetlenmiştir. Anahtar KelimelerDNA, DNA ayrılması, DNA saflaştırılması, SPE. A B S T R A C T DNA isolation/purification is a widely used procedure for molecular biology, medical studies, gene therapy and especially PCR studies which needs high quality or extremely pure nucleic acid samples. Nucleic acid separation was mainly achieved by two methods; solvent extraction and solid phase extraction (SPE) methods. Although solvent extraction method is the simplest method, there are some drawbacks such as toxicity, more solvent requirement, time-consuming procedure and low specificity. Nowadays, there is a wide use of solid phase matrices to extract DNA from a variety of samples. In this review article; we basically focused on SPE for DNA purification methods.

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Dye affinity cryogels for plasmid DNA purification

Cited by 30 publications

References 63 publications

Injectable Cryogels in Biomedicine

Injectable Cryogels in Biomedicine

Current State of the Art in DNA Vaccine Delivery and Molecular Adjuvants: Bcl-xL Anti-Apoptotic Protein as a Molecular Adjuvant

DNA Purification by Solid Phase Extraction (SPE) Methods

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