Dye-doped nanoparticles for bioanalysis

Yan, Jilin; Estévez, M.‐Carmen; Smith, Joshua E.; Wang, Kemin; He, Xiaoxiao; Wang, Lin; Tan, Weihong

doi:10.1016/s1748-0132(07)70086-5

Cited by 366 publications

(261 citation statements)

References 64 publications

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“…Dye-doped silica nanoparticles [13][14][15] (NP) stand out as excellent candidates as it is possible to dope silica NPs with a large number of fluorophores, increasing the total fluorescence of the label significantly [16,17]. Moreover, the fluorophore is protected inside a silica matrix, thereby increasing photostability [18,19] and quantum efficiency [20,21].…”

mentioning

confidence: 99%

A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performance

Gubala

Guével

Nooney

et al. 2010

Talanta

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When designing devices for biomedical diagnostics, increasing the signal to noise ratio is often critical for achieving clinically relevant sensitivity and limits of detection (LOD). In antibodybased assays, the measured signal can be amplified through the replacement of molecular fluorophores with doped nanoparticles (NP). However, the benefits of using NPs can only be realized if the NPs are coated efficiently with detection antibody, have good colloidal stability and the ratio of specific to non-specific binding (NSB) is high enough. The main focus of this paper is on the optimization of the bioconjugation protocol for antibody labeling of NPs leading to improved assay performance. Two types of linkers were used: monovalent linkers (glutaraldehyde; sulfo-SMCC; and sulfo-SIAB), and three generations of dendrimers endowed with multivalent carboxylic functionality. Overall, the NP-IgG conjugates prepared using multivalent linkers showed a significantly lower LOD and higher sensitivity than their homo-or hetero-functional counterparts. The multivalent dendrimers also improved NP stability and reduced aggregation. Moreover, the dendrimers showed a higher reactivity with biological material, a feature that could significantly reduce the cost of high-throughput biodiagnostics tests. Introduction:

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confidence: 99%

A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performance

Gubala

Guével

Nooney

et al. 2010

Talanta

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“…As stated above, there are a range of different Silicabased nanoparticles, but for the purpose of this review it will be limited to the ones that have been researched in reference to bacterial diagnostics, just to note there is a great summary of most of the silica based nanoparticles being used in bioimaging in a review by Yan [38]. In this review it will be mainly focusing on the use of dye-doped silica nanoparticles will be mainly focussed, due to the fact that there has been a considerable amount of research conducted on them and that they have been observed to be quite effective.…”

Section: Dye-doped Fluorescent Silica Nanoparticlesmentioning

confidence: 99%

Applications of Nanomedicine in Antibacterial Medical Therapeutics and Diagnostics~!2009-08-26~!2009-11-25~!2010-02-24~!

Matthews¹,

Kanwar²,

Zhou³

et al. 2010

TOTMJ

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Abstract:The need for new and effective/efficient antibacterial therapeutics and diagnostics is necessary if we want to be able to maintain and improve the protection against pathogenic bacteria. Bacteria are becoming increasingly resistant to traditionally used antibiotics and as a result are a major health concern. The number of deaths and hospitalizations due to bacteria is increasing. Current methods of bacterial diagnostics are inefficient as they lack speed and ultra sensitivity and cannot be performed on site. This is where nanomedicine is playing a vital role. The discovery of new and innovative materials through the improvement in fabrication techniques has seen the establishment of an influx of novel antibacterial therapeutics and diagnostics. The goal of this review is to highlight the research that has been done through the implementation of nanomaterials and nanotechnologies for antibacterial medical therapeutic and diagnostic.

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“…[18][19][20][21][22][23][24] Starting from porous silica, the synthesis of organic-inorganic hybrids can be achieved by different methods: the post synthesis impregnation of the matrix or the introduction of dye molecules during the sol phase (pre-synthesis) [25][26][27]. Depending on the bond between the guest molecules and the host surface, the hybrids are divided into two categories: type I, where the bond is weak (van der Walls interaction or H-bond), and type II, where the bond is covalent.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence properties of dye doped mesoporous silica

Carbonaro¹,

Corpino²,

Ricci³

et al. 2014

AIP Conference Proceedings

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Abstract. In this paper we present a review of the main results we obtained studying the emission properties of organicinorganic hybrids obtained combining mesoporous silica and Xantene dyes, in particular the standard referenc Rhodamine 6G. The purpose of the review is to show the possibility to efficiently "dope" the transparent inorganic porous matrix to obtain promising systems for photonic and biomedical applications. The strategies to solve the concentration effect and the leaching phenomenon are discussed within the framework of the single exciton theory.

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Dye-doped nanoparticles for bioanalysis

Cited by 366 publications

References 64 publications

A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performance

A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performance

Applications of Nanomedicine in Antibacterial Medical Therapeutics and Diagnostics~!2009-08-26~!2009-11-25~!2010-02-24~!

Fluorescence properties of dye doped mesoporous silica

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