Dynabeads protein G antibody conjugates combined with modified brain heart infusion broth for the enrichment and separation of Bacillus cereus in artificially contaminated vegetables

Wei, Shuai; Forghani, Fereidoun; Park, Youn-Seo; Park, Byung‐Jae; Seo, Kun‐Ho; Oh, Deog‐Hwan

doi:10.1007/s10068-016-0154-z

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…Pathogens Reference Meat S. enterica, E. coli O157:H7, Brucella melitensis, L. monocytogenes, Shigella spp., S. aureus and C. jejuni (Vinayaka et al, 2019;You et al, 2021;Ma et al, 2014;Bruno et al, 2009) Vegetables and fruits L. monocytogenes, S. enterica, S. aureus, B. cereus and E. coli O157:H7 (X. Huang et al, 2015;Huang, Mahboubat, et al, 2021;Wei, Forghani, et al, 2016;Ozalp et al, 2013;Y. Wang, Zhang, et al, 2022) Dairy products S. enterica, E. coli O157:H7, Brucella melitensis, L. monocytogenes, Cronobacter spp., S. aureus, Shigella spp.…”

Section: Food Typesmentioning

confidence: 99%

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Xiao

2022

Comp Rev Food Sci Food Safe

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Foodborne pathogens contamination is one of the main sources of food safety problems. Although the existing detection methods have been developed for a long time, the complexity of food samples is still the main factor affecting the detection time and sensitivity, and the rapid separation and enrichment of pathogens is still an objective to be studied. Magnetic separation strategy based on magnetic nanoparticles (MNPs) is considered to be an effective tool for rapid separation and enrichment of foodborne pathogens in food. Therefore, this study comprehensively reviews the development of MNPs in the separation of foodborne pathogens over the past decade. First, various biorecognition reagents for identification of foodborne pathogens and their modifications on the surface of MNPs are introduced. Then, the factors affecting the separation of foodborne pathogens, including the size of MNPs, modification methods, separation strategies and separation forms are discussed. Finally, the application of MNPs in integrated detection methods is reviewed. Moreover, current challenges and prospects of MNPs for the analysis of foodborne pathogens are discussed. Further research should focus on the design of multifunctional MNPs, the processing of large‐scale samples, the simultaneous analysis of multiple targets, and the development of all‐in‐one small analytical device with separation and detection.

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Section: Food Typesmentioning

confidence: 99%

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Xiao

2022

Comp Rev Food Sci Food Safe

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“…Immunomagnetic separation (IMS) methods use immunomagnetic beads as the capture reagents to isolate pathogens from food samples. IMS beads have antibodies attached to their surface and are complementary methods used to improve sensitivity and/or specificity of detection protocols (17). In food safety detection, one of the first successful applications of IMS was to dramatically increase the recovery of E. coli O157:H7 from food and cattle fecal samples, revealing a markedly greater prevalence in bovine populations (18).…”

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confidence: 99%

Salmonella enterica and Escherichia coli in Wheat Flour: Detection and Serotyping by a Quasimetagenomic Approach Assisted by Magnetic Capture, Multiple-Displacement Amplification, and Real-Time Sequencing

Forghani

Zhang

et al. 2020

Appl Environ Microbiol

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Food safety is a new area for novel applications of metagenomics analysis, which not only can detect and subtype foodborne pathogens in a single workflow but may also produce additional information with in-depth analysis capabilities. In this study, we applied a quasimetagenomic approach by combining short-term enrichment, immunomagnetic separation (IMS), multiple-displacement amplification (MDA), and nanopore sequencing real-time analysis for simultaneous detection of Salmonella and Escherichia coli in wheat flour. Tryptic soy broth was selected for the 12-h enrichment of samples at 42°C. Enrichments were subjected to IMS using beads capable of capturing both Salmonella and E. coli. MDA was performed on harvested beads, and amplified DNA fragments were subjected to DNA library preparation for sequencing. Sequencing was performed on a portable device with real-time basecalling adaptability, and resulting sequences were subjected to two parallel pipelines for further analysis. After 1 h of sequencing, the quasimetagenomic approach could detect all targets inoculated at approximately 1 CFU/g flour to the species level. Discriminatory power was determined by simultaneous detection of dual inoculums of Salmonella and E. coli, absence of detection in control samples, and consistency in microbial flora composition of the same flour samples over several rounds of experiments. The total turnaround time for detection was approximately 20 h. Longer sequencing for up to 15 h enabled serotyping for many of the samples with more than 99% genome coverage, which could be subjected to other appropriate genetic analysis pipelines in less than a total of 36 h. IMPORTANCE Enterohemorrhagic Escherichia coli (EHEC) and Salmonella are of serious concern in low-moisture foods, including wheat flour and its related products, causing illnesses, outbreaks, and recalls. The development of advanced detection methods based on molecular principles of analysis is essential to incorporate into interventions intended to reduce the risk from these pathogens. In this work, a quasimetagenomic method based on real-time sequencing analysis and assisted by magnetic capture and DNA amplification was developed. This protocol is capable of detecting multiple Salmonella and/or E. coli organisms in the sample within less than a day, and it can also generate sufficient whole-genome sequences of the target organisms suitable for subsequent bioinformatics analysis. Multiplex detection and identification were accomplished in less than 20 h and additional whole-genome analyses of different nature were attained within 36 h, in contrast to the several days required in previous sequencing pipelines.

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Rapid and accurate quantification of viable Bacillus cereus in foods with a Propidium monoazide (PMA) - Fluorescence in situ hybridization (FISH) - Flow cytometry (FCM) method

Bai,

Wang,

Xie

et al. 2023

LWT

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Dynabeads protein G antibody conjugates combined with modified brain heart infusion broth for the enrichment and separation of Bacillus cereus in artificially contaminated vegetables

Cited by 4 publications

References 32 publications

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

Salmonella enterica and Escherichia coli in Wheat Flour: Detection and Serotyping by a Quasimetagenomic Approach Assisted by Magnetic Capture, Multiple-Displacement Amplification, and Real-Time Sequencing

Rapid and accurate quantification of viable Bacillus cereus in foods with a Propidium monoazide (PMA) - Fluorescence in situ hybridization (FISH) - Flow cytometry (FCM) method

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