Dynamic aspects of contact angle measurements on adsorbed protein layers

Scheer, A. Van Der; Smolders, C.A.

doi:10.1016/0021-9797(78)90029-2

Cited by 26 publications

(2 citation statements)

References 16 publications

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“…It appears that the influence on the final 0, values is very small. In a previous paper [2] we derived an equation to estimate the value of the polar interaction term I, ( p ) w r between an adsorbed protein layer and the aquedus phase. It was assumed that no turnover of the protein molecules from the PS substrate to the PO/water interface takes place.…”

Section: The Effect Of Ph and Salt On Results For Hsa Coatingsmentioning

confidence: 99%

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Adsorbed layers of albumin and fibrinogen on polystyrene, probed by contact angle measurements

Scheer

Bargeman

Koetsier

et al. 1979

J. polym. sci., C Polym. symp.

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SYNOPSISEarlier measurements of paraffin oil (PO)-water contact angles on protein layers [human serum albumin (HSA) or human fibrinogen (HFb)] adsorbed on polystyrene (PS) showed that HSA could be transferred from the PSlwater to the POlwater interface by a passing POwater front, while HFb could not. Interfacial association of adsorbed HF% molecules was adopted as an explanation for the irreversible localization of HFb at the PS surface. In the present work it is shown that aggregation by a heat treatment of adsorbed HSA molecules also causes an irreversible localization of the HSA at the PS surface. It is shown that advancing and receding PO-water contact angles on HSA-coated PS substrates have practically the same value. Variation of pH and NaCl concentration hardly shows any effect on these contact angles, indicating that in all cases the HSA coating behaves the same. The advancing and receding PO-water contact angles on HFb-coated substrates differ greatly. In analogy with ElShimi and Goddard we conclude that the natural HF% coating is able to adopt and retain a configuration compatible with the immediate environment.

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Section: The Effect Of Ph and Salt On Results For Hsa Coatingsmentioning

confidence: 99%

“…In a previous paper [2] we have worked out protein layer wettability in a more quantitative way. It was shown that the behavior of an oil drop on a protein-coated polystyrene (PS) substrate immersed in buffer solution depends on the type of protein used for the coating.…”

Section: Introductionmentioning

confidence: 99%

Adsorbed layers of albumin and fibrinogen on polystyrene, probed by contact angle measurements

Scheer

Bargeman

Koetsier

et al. 1979

J. polym. sci., C Polym. symp.

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The interaction of plasma proteins with polymers. I. Relationship between polymer surface energy and protein adsorption/desorption

Baszkin

Lyman

1980

J. Biomed. Mater. Res.

193

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Adsorption of bovine albumin, gamma-globulin and fibrinogen from phosphate buffer solution (pH = 7.5) onto several polymer films was studied using the radioiodinated proteins (125I). The kinetics of desorption of the proteinated polymer films in bovine plasma was determined. Contact angle measurements on these same polymers allowed the calculation of dispersive (WA d) and polar (Ip) components of the polymer-protein solution system. Results from these measurements show that the nondispersive-dispersive force balance at the polymer-protein solution interface, expressed by the Ip/WA d ratio, is an important factor for binding of proteins on polymer surfaces. The purity of fibrinogen and the cleaning procedures for the polymer surfaces influence the absolute values of proteins adsorbed on polymer surfaces.

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Dispersion and polar surface free energies of human enamel

Pelt

Jong

Busscher

et al. 1983

J. Biomed. Mater. Res.

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In this study, the dispersion (gamma ds) and polar (gamma ps) surface free energies of human enamel with and without an acquired enamel pellicle and of crystalline hydroxyapatite were determined from contact angle measurements. The results show that gamma ps is strongly influenced by the presence of a pellicle; the total surface free energy (gamma s) is considerably smaller for enamel with an acquired pellicle than for enamel without an acquired pellicle. Enamel without an acquired enamel pellicle shows surface free energies comparable with those of crystalline hydroxyapatite.

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Dynamic aspects of contact angle measurements on adsorbed protein layers

Cited by 26 publications

References 16 publications

Adsorbed layers of albumin and fibrinogen on polystyrene, probed by contact angle measurements

Adsorbed layers of albumin and fibrinogen on polystyrene, probed by contact angle measurements

The interaction of plasma proteins with polymers. I. Relationship between polymer surface energy and protein adsorption/desorption

Dispersion and polar surface free energies of human enamel

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