Dynamic changes in the free and bound phenolic compounds and antioxidant activity of brown rice at different germination stages

Ti, Huihui; Zhang, Ruifen; Zhang, Mingwei; Li, Qing; Wei, Zhencheng; Zhang, Yan; Tang, Xiaojun; Deng, Yuanyuan; Liu, Lei; Ma, Yongxuan

doi:10.1016/j.foodchem.2014.04.024

Cited by 176 publications

(141 citation statements)

References 25 publications

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“…Phenolic acids were extracted using a method reported previously [22], with some modifications. Briefly, the residue from the free phenolic extraction was hydrolyzed with 100 mL of 4 M NaOH at 60˝C with continuous stirring for 4 h. The mixture was centrifuged at 5000 rpm for 10 min and was filtrated by filter papers.…”

Section: Extraction Of Phenolic Acidsmentioning

confidence: 99%

Involvement of Secondary Metabolites in Response to Drought Stress of Rice (Oryza sativa L.)

Quan

Anh²,

Khang³

et al. 2016

Agriculture

104

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Abstract:In this study, responses of rice under drought stress correlating with changes in chemical compositions were examined. Among 20 studied rice cultivars, Q8 was the most tolerant, whereas Q2 was the most susceptible to drought. Total phenols, total flavonoids, and antioxidant activities, and their accumulation in water deficit conditions were proportional to drought resistance levels of rice. In detail, total phenols and total flavonoids in Q8 (65.3 mg gallic acid equivalent (GAE) and 37.8 mg rutin equivalent (RE) were significantly higher than Q2 (33.9 mg GAE/g and 27.4 mg RE/g, respectively) in both control and drought stress groups. Similarly, the antioxidant activities including DPPH radical scavenging, β-carotene bleaching, and lipid peroxidation inhibition in Q8 were also higher than in Q2, and markedly increased in drought stress. In general, contents of individual phenolic acids in Q8 were higher than Q2, and they were significantly increased in drought stress to much greater extents than in Q2. However, p-hydroxybenzoic acid was found uniquely in Q8 cultivars. In addition, only vanillic acid was found in water deficit stress in both drought resistant and susceptible rice, suggesting that this phenolic acid, together with p-hydroxybenzoic acid, may play a key role in drought-tolerance mechanisms of rice. The use of vanillic acid and p-hyroxybenzoic acid, and their derivatives, may be useful to protect rice production against water shortage stress.

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Section: Extraction Of Phenolic Acidsmentioning

confidence: 99%

Involvement of Secondary Metabolites in Response to Drought Stress of Rice (Oryza sativa L.)

Quan

Anh²,

Khang³

et al. 2016

Agriculture

104

View full text Add to dashboard Cite

show abstract

“…The total phenolic content was estimated based on the Folin-Ceiocalteu method as following the procedure detailed in Ti et al [9]. A mixture of 0.1 ml extract, 0.5 ml of Folin-Ceiocalteu 10% and 0.4 ml Na 2 CO 3 7.5% was mixed in a microtube and incubated for 30 min at room temperature.…”

Section: Estimation Of Total Phenolic Contentmentioning

confidence: 99%

Involvement of Phenolic Compounds in Anaerobic Flooding Germination of Rice (<i>Oryza sativa</i> L.)

Khang

Lang

et al. 2016

ILNS

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By this study, thirty rice varieties were evaluated for anaerobic flooding tolerance using the direct sowing method. Phenolic profiles of strong and weak tolerant varieties were identified and compared based on HPLC chromatograms. The germination rates and shoot heights of rice were recorded for calculating the seedling vigor, which indicate the tolerant ability of rice in flooding condition. The results revealed a high variation of germination rate (10.01 to 100%), shoot height (0.35 to 78.17 mm) and seedling vigor (0.05 to 72.83). There was a high correlation between (r = 0.71) germination rate in 5 cm and 10 cm flood. Phenolic and flavonoid contents of the strong tolerant cultivar significantly and proportionally increased in the flooding levels (5 cm and 10 cm). There was a total difference in terms of number of phenolic acids found in the strong and weak tolerant varieties. In particular, six phenolic acids (gallic acid, catechol, caffeic acid, syringic acid, vanillin, and ellagic acid) were only identified with high concentration in the strong tolerant cultivar. The findings suggest that the phenolics presented in the strong tolerant varieties probably have a certain function in response and adaptation to anaerobic flooding condition. Further researches on exogenous application of these phenolic acids to increase the flooding tolerant level of rice should be continued at both green house and field treatments.

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“…Recently, this trend has dramatically changed that the plenty of beneficial substances in germinated brown rice has considerably captured international attention. Phenolics, one of the most abundant compounds of active phytochemicals in dehulled rice, are empirically examined to increase during germination [23]. In addition, in many previous studies, the crucial role of phenolics in allelopathy was demonstrated [10,20,[24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Allelopathic Activity of Dehulled Rice and its Allelochemicals on Weed Germination

Khang

Anh

et al. 2016

ILNS

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In this study, the allelopathic potential of dehulled rice, rice, and hulls of rice on germination of weeds was evaluated in laboratory. Phenolic acids in growth media of these plants were also identified and quantified using HPLC. Identified allelochemicals were reversely tested for phytotoxic effects on germination and seedling growth of barnyardgrass, lettuce, radish and rice. The results showed that the inhibitory effects of dehulled rice were stronger than those of hulls and rice. Dehulled rice caused 66.7% and 50.6% reduction in radish root length and lettuce shoot height, respectively. Dehulled rice showed strong inhibitory effects on root length of lettuce and shoot height of radish while hulls and rice exhibited stimulation. Regarding phenolic identification, vanillin and vanillic acid were detected as allelochemicals in root exudates of rice and tested plants. The results revealed that most of the vanillin and vanillic acid treatments showed high inhibitory effects on germination rates and seedling growth of lettuce and radish, high stimulatory activity on root elongation of rice. Vanillic acid (100 ppm and 200 ppm), vanillin (100 ppm) and their mixture (200 ppm) completely inhibited survival of lettuce. The findings indicate the allelopathic potential of dehulled rice which can be used for identification of more phytotoxins to produce bioherbicides in agricultural practices.

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Dynamic changes in the free and bound phenolic compounds and antioxidant activity of brown rice at different germination stages

Cited by 176 publications

References 25 publications

Involvement of Secondary Metabolites in Response to Drought Stress of Rice (Oryza sativa L.)

Involvement of Secondary Metabolites in Response to Drought Stress of Rice (Oryza sativa L.)

Involvement of Phenolic Compounds in Anaerobic Flooding Germination of Rice (<i>Oryza sativa</i> L.)

Allelopathic Activity of Dehulled Rice and its Allelochemicals on Weed Germination

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