Dynamic Coating Agents in CE

Znaleziona, Joanna; Petr, Jan; Knob, Radim; Maier, Vítězslav; Ševčı́k, Juraj

doi:10.1365/s10337-007-0509-y

Cited by 62 publications

(56 citation statements)

References 116 publications

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“…In spite of the large amount of experimental work [13][14][15][16] on the mechanisms controlling the EOF in polymer coated capillaries, a complete understanding of the problem is still missing [17]. The scaling theory of Harden et al [18] focused on the interplay between the deformation of the adsorbed polyelectrolytes and the fluid motion.…”

Section: Introductionmentioning

confidence: 99%

Electro-osmotic flow in coated nanocapillaries: a theoretical investigation

Marconi

Monteferrante

Melchionna

2014

Phys. Chem. Chem. Phys.

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Motivated by recent experiments, we present a theoretical investigation of how the electro-osmotic flow occurring in a capillary is modified when its charged surfaces are coated by charged polymers. The theoretical treatment is based on a three dimensional model consisting of a ternary fluid-mixture, representing the solvent and two species for the ions, confined between two parallel charged plates decorated by a fixed array of scatterers representing the polymer coating. The electro-osmotic flow, generated by a constant electric field applied in a direction parallel to the plates, is studied numerically by means of Lattice Boltzmann simulations. In order to gain further understanding we performed a simple theoretical analysis by extending the Stokes-Smoluchowski equation to take into account the porosity induced by the polymers in the region adjacent the walls. We discuss the nature of the velocity profiles by focusing on the competing effects of the polymer charges and the frictional forces they exert. We show evidence of the flow reduction and of the flow inversion phenomenon when the polymer charge is opposite to the surface charge. By using the density of polymers and the surface charge as control variables, we propose a phase diagram that discriminates the direct and the reversed flow regimes and determine its dependence on the ionic concentration.

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Section: Introductionmentioning

confidence: 99%

Electro-osmotic flow in coated nanocapillaries: a theoretical investigation

Marconi

Monteferrante

Melchionna

2014

Phys. Chem. Chem. Phys.

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“…Due to their equilibrium based nature, these coatings need to be continuously regenerated by refreshing the adsorbed layer on the capillary wall [1]. Dynamic coating materials reversibly adsorb onto the capillary surface with the advantage of easy removal and regeneration if necessary [11]. The most commonly used agents are either small ions, like amines or oligoamines, anionic and cationic surfactants (sodium dodecyl sulfate -SDS, cetyltrimethylammonium bromide -CTAB, etc.)…”

Section: Dynamic Coating Approachesmentioning

confidence: 99%

Recent advances in column coatings for capillary electrophoresis of proteins

Hajba

Guttman

2017

TrAC Trends in Analytical Chemistry

121

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Capillary coatings effectively improve the separation performance of proteins in capillary electrophoresis, mainly by reducing protein adsorption onto the inner capillary wall and by regulating the electroosmotic flow (EOF) to accommodate the separation problem in hand. In the first part of this review the newest trends in dynamic and permanent capillary coatings are summarized and discussed in detail. In the second part the application of nanomaterials as novel capillary coating materials is conversed. Nanomaterials have great potential in capillary coating preparations based on their advantageous properties such as large surface-to-volume ratios and a wide variety of chemistry options. Finally, some future prospective of capillary coatings in the emerging field of proteomics are given.

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“…Another strategy for capillary coating to eliminate EOF other than "permanent" coating through covalent bonding is the dynamic coating method, [14][15][16] and this method is quite promising from a practical point of view. The use of dynamic coating can significantly improve the routine performance of CE and the repeatability of migration time between capillaries.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

et al. 2013

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A simple, rapid, and precise dynamic coating capillary electrophoretic separation method for water-soluble humic substances is proposed. An aqueous solution containing hydroxyethyl cellulose (HEC), polyethylene glycol (PEG), and pH buffer component was employed for both the dynamic coating agent and the separation medium. The procedure for the coating of the capillary inner wall was simply filling the buffered polymer mixture solution into the capillary that had been treated with 1 M aqueous HC1 solution. The solution for the capillary coating was directly used as the electrophoretic buffer solution for CE separation. Excellent performance for the separation of humic acid was obtained using the solution containing 0.5% (w/v) HEC, 1.0% (w/v) PEG 10000, and 0.1% (w/v) PEG 8000000. Excellent reproducibility and durability were obtained even at slightly alkaline conditions at pH levels above 8. The separation of 0.1 - 2 kbp of DNA ladder by the proposed method showed was also achieved

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Dynamic Coating Agents in CE

Cited by 62 publications

References 116 publications

Electro-osmotic flow in coated nanocapillaries: a theoretical investigation

Electro-osmotic flow in coated nanocapillaries: a theoretical investigation

Recent advances in column coatings for capillary electrophoresis of proteins

Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

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