Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

Abstract

A simple, rapid, and precise dynamic coating capillary electrophoretic separation method for water-soluble humic substances is proposed. An aqueous solution containing hydroxyethyl cellulose (HEC), polyethylene glycol (PEG), and pH buffer component was employed for both the dynamic coating agent and the separation medium. The procedure for the coating of the capillary inner wall was simply filling the buffered polymer mixture solution into the capillary that had been treated with 1 M aqueous HC1 solution. The solution for the capillary coating was directly used as the electrophoretic buffer solution for CE separation. Excellent performance for the separation of humic acid was obtained using the solution containing 0.5% (w/v) HEC, 1.0% (w/v) PEG 10000, and 0.1% (w/v) PEG 8000000. Excellent reproducibility and durability were obtained even at slightly alkaline conditions at pH levels above 8. The separation of 0.1 - 2 kbp of DNA ladder by the proposed method showed was also achieved

Recent trends in capillary electrophoresis for complex samples analysis: A review

“…On the other hand, a mixture of hydroxyethyl cellulose and PEG has been employed as dynamic coating for the determination of humic acid in natural water samples . The obtained coating in this case, acted as an EOF suppressor and as a molecular sieve (similar to that achieved by capillary gel electrophoresis), allowing the analysis of humic substances at the weakly alkaline mediums usually found in natural waters.…”

Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009–2014)

“…A blended hydroxyethylcellulose matrix consisting of 0.21 % 27-kDa and 0.07 % 1-MDa hydroxyethylcellulose with 0.12 % 7-MDa linear polyacrylamide was used for the separation of DNA fragments ranging from 200 to 40,000 bases in 2 min in a glass microfluidic coated with polyhydroxyethylacrylamide [ 77 ]. The poor surface passivation by hydroxyethylcellulose can be overcome by blending other effective surface coating agents such as polyethylene glycol [ 78 ], polyvinyl alcohol [ 79 ], and polydimethylacrylamide [ 79 ].…”