Dynamic colloidal nanoparticle assembly triggered by aptamer–receptor interactions on live cell membranes

Yang, Linlin; Meng, Lingyan; Song, Jiaying; Xiao, Yue; Wang, Ruowen; Kang, Houjun; Han, Da

doi:10.1039/c9sc02693b

Cited by 17 publications

(13 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Activated by a target molecule, the DNA walker can automatically move along a predesigned track driven by DNAzymes, nicking endonuclease, and strand displacement. 28 Signal amplification and functional biological operation are achieved with alternating responses and reversible interactions. In particular, the three-dimensional (3D) walking nanomachines assembled into external nanoparticles are favorable in terms of signal enhancement due to the greater mobility and load capacity.…”

mentioning

confidence: 99%

“…The high specificity and programmability of hybridization make DNA nanodevices a compelling tool for signal amplification and logical assembly. A variety of DNA nanomachines, such as DNA tweezers, DNA robots, molecular motors, and DNA walkers, − have been constructed and applied to biological analysis. Among these nanomachines, the DNA walker, as an attractive signal enhancement circuit, has received great attention.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Many Birds, One Stone: A Smart Nanodevice for Ratiometric Dual-Spectrum Assay of Intracellular MicroRNA and Multimodal Synergetic Cancer Therapy

Han

et al. 2021

ACS Nano

View full text Add to dashboard Cite

The development of a theragnostic platform integrating precise diagnosis and effective treatment is significant but still extremely challenging. Herein, an integrated smart nanodevice composed of Au@Cu2–x S@polydopamine nanoparticles (ACSPs) and fuel DNA-conjugated tetrahedral DNA nanostructures (fTDNs) was constructed, in which the ACSP nanoprobe played multiple key roles in antitumor therapy as well as in situ monitoring of microRNAs (miRNAs) in cancer cells. Regarding the analysis, the ACSP probe contained two optical properties: excellent surface-enhanced Raman scattering (SERS) enhancement and high fluorescence (FL) quenching performance. Employing the ACSPs as the high-efficiency detection substrate combined with the fTDN-assisted DNA walking nanomachines as the superior amplification strategy, a SERS-FL dual-spectrum biosensor was constructed, which achieved an ultralow background signal and excellent sensitivity with detection limits of 0.11 pM and 4.95 aM by FL and SERS, respectively. Moreover, the rapid FL imaging and precise SERS quantitative detection for miRNA in cancer cells were also achieved by dual-signal ratio strategy, improving the accuracy of diagnosis. Regarding the therapeutic application, due to the high reactive oxygen species generation ability and excellent photothermal conversion efficiency, the ACSPs can also act as an all-in-one nanoagent for multimodal collaborative tumor therapy. Significantly, both in vivo and in vitro experiments confirmed its high biological safety and strong anticancer effect, indicating its promising theragnostic applications.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Many Birds, One Stone: A Smart Nanodevice for Ratiometric Dual-Spectrum Assay of Intracellular MicroRNA and Multimodal Synergetic Cancer Therapy

Han

et al. 2021

ACS Nano

View full text Add to dashboard Cite

show abstract

“…Combination of these two modules enables the quick and sensitive detection on a broad range of targets. Han et al [304] developed a DNA displacement reaction-based dynamic system with an aptamer in which the nanostructure can be transformed in response to the epithelial cell adhesion molecule (EpCAM) on live cell membranes and propagate signals through DNAbased signal amplification reactions. Overall, the unintegrated method that involves multiple molecules for signal Figure 11 Strategies for nucleic acid-based bioanalysis.…”

Section: Signal Recognition and Transduction Strategiesmentioning

confidence: 99%

Nucleic Acids Analysis

et al. 2020

Self Cite

View full text Add to dashboard Cite

In recent years, multi-modal entity linking (MEL) has garnered increasing attention in the research community due to its significance in numerous multi-modal applications. Video, as a popular means of information transmission, has become prevalent in people's daily lives. However, most existing MEL methods primarily focus on linking textual and visual mentions or offline videos's mentions to entities in multi-modal knowledge bases, with limited efforts devoted to linking mentions within online video content. In this paper, we propose a task called Online Video Entity Linking (OVEL), aiming to establish connections between mentions in online videos and a knowledge base with high accuracy and timeliness. To facilitate the research works of OVEL, we specifically concentrate on live delivery scenarios and construct a live delivery entity linking dataset called LIVE. Besides, we propose an evaluation metric that considers timelessness, robustness, and accuracy. Furthermore, to effectively handle OVEL task, we leverage a memory block managed by a Large Language Model and retrieve entity candidates from the knowledge base to augment LLM performance on memory management. The experimental results prove the effectiveness and efficiency of our method.

show abstract

“…Furthermore, by mimicking biomacromolecules, such PANs can be used as building bricks to assemble into more complex and ordered three-dimensional (3D) supramolecular materials (e.g., superlattices, supercrystals, etc) by deliberate design and a series of hybridization reactions. [8][9][10][11][12][13][14] These products feature exotic properties and functions, which have been used in imaging, sensing, therapeutics, and so forth. 2,[15][16][17][18] So far, a variety of DNA structures including thiolated DNA, spherical nucleic acids, DNA tile, DNA origami and DNA patches have been served as "glue" sticky to nanoparticles for valence engineering of PANs.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic synthesis of high-valence programmable atom-like nanoparticles for reliable sensing

Shi

Chen

et al. 2021

Chem. Sci.

View full text Add to dashboard Cite

show abstract

Dynamic colloidal nanoparticle assembly triggered by aptamer–receptor interactions on live cell membranes

Abstract: We engineered an aptamer and DNA displacement reaction-based dynamic system that can transform its shape in response to epithelial cell adhesion molecule (EpCAM) on live cell membranes.

Cited by 17 publications

References 38 publications

Many Birds, One Stone: A Smart Nanodevice for Ratiometric Dual-Spectrum Assay of Intracellular MicroRNA and Multimodal Synergetic Cancer Therapy

Many Birds, One Stone: A Smart Nanodevice for Ratiometric Dual-Spectrum Assay of Intracellular MicroRNA and Multimodal Synergetic Cancer Therapy

Nucleic Acids Analysis

Microfluidic synthesis of high-valence programmable atom-like nanoparticles for reliable sensing

Contact Info

Product

Resources

About