Dynamic Comparison of Free Radical Scavenging Abilities of Hypericum Perforatum L., Herba Verbenae Officinalis, and Valeriana Officinalis L. Extracts

Abstract

Objectives: The purpose of this research was to compare the dynamic antioxidant activities and the free radical scavenging abilities of three botanical supplements, Hypericum perforatum L. (HPL), Herba Verbena Officinalis (HVO), and Valeriana officinalis L. (VO), which have been reported to effectively treat menopause symptoms. Methods: The antioxidant activities of the three supplements were determined by the ferric reducing ability of plasma (FRAP) assay. In addition, their free radical scavenging abilities were studied by ftheir interactions with the stable radicals of 2, 2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). Results: The results indicated that the HPL ethanolic extract exhibited greater antioxidant activity (1.2688 ± 0.01 mmol Trolox/g) than the HVO ethanolic extract (1.1686± 0.043 mmol Trolox/g) and the VO ethanolic extract (0.2579 ± 0.0031 mmol Trolox/g) as compared to Nilestriol (0.0026±0.0012), a positive control agent in the experiment. Moreover, the HPL extract showed remarkable free radical scavenging activity against ABTS•+, and the HVO extract was the most potent against DPPH•. Conclusion: These activities may be attributed to the total flavonoid or phenolic acid contents of among these extracts.

Updating the biological interest of 'Valeriana officinalis'

“…Moreover, 80% methanolic extract showed the highest DPPH radical scavenging activity. In another study, V. officinalis ethanolic extracts (95%) was effective to scavenge DPPH radical (Li et al, 2015). This antioxidant activity was positively correlated with flavonoids and tannin content (Pilerood &Prakash, 2014;Li et al, 2015).…”

“…In another study, V. officinalis ethanolic extracts (95%) was effective to scavenge DPPH radical (Li et al, 2015). This antioxidant activity was positively correlated with flavonoids and tannin content (Pilerood &Prakash, 2014;Li et al, 2015). On the other hand, V. officinalis ethanol extracts (40%) showed low antioxidant activity in ORAC and HORAC assays (Katsarova et al, 2018).…”

“…All these works focused on studying antioxidant and neuroprotective activity, except one which aims to evaluate its antidiabetic properties. Four of these studies used chemical in vitro assays to measure antioxidant capacity and acetylcholinesterase inhibitory activity (Pilerood & Prakash, 2014;Li et al, 2015;Chen et al, 2016;Katsarova et al, 2018). In the other two in vitro studies, cell lines have been employed, particularly, the human neuroblastoma SH-SY5Y cells and the mouse 3T3-L1 preadipocytes (Gonulalan et al, 2018;Harada et al, 2020).…”

Protective Role of Hypericum perforatum L. and Hypericum triquetrifolium Turra against Inflammatory Diseases: Evidence from In vitro and In vivo Studies

“…The ethanolic extracts of H. perforatum had a gradual but consistent level of dynamic DPPH and ABTS scavenging activity. The amount of total flavonoids or phenolic acids present could play an important role in the antioxidant activity and free radical scavenging activity [68]. A study conducted in Syria on the aerial parts of St. John's wort -leaves, stems, petals, and flowersfound that water, ethanol, methanol, and acetone extracts were all efficient scavengers of ABTS and DPPH stable free radicals.…”