Dynamic dentin: A quantitative microscopic assessment of age and spatial changes to matrix architecture, peritubular dentin, and collagens types I and III

Weerakoon, A.; Condon, Nicholas D.; Cox, Thomas R.; Sexton, Christopher; Cooper, Crystal; Meyers, Ian; Thomson, David; Ford, Pauline; Roy, S.; Symons, A. L.

doi:10.1016/j.jsb.2022.107899

Cited by 6 publications

(5 citation statements)

References 54 publications

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“…Picrosirius red staining enables qualitative and quantitative assessment of collagen distribution, maturity, and fibrillar packing through collagen birefringence. Polarizing microscopy facilitates the visualization of anisotropic structures that appear bright or glossy on a dark background [29].…”

Section: Discussionmentioning

confidence: 99%

The effect of the irrigation solutions on dentin organic components: Pilot study

Khabadze,

Generalova,

Taptun

et al. 2024

Èndodontiâ today

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INTRODUCTION. Tooth decay and its complications are prevalent dental issues among adults. Endodontic treatment is the standard of care for complicated caries, specifically pulpitis and periodontitis. Endodontic irrigants, which are placed in the root canal lumen for a specific duration, can affect the intracanal dentin structure (organic and inorganic substances) in various ways. The use of polyhexanide-based antiseptic products in endodontics is a promising idea to overcome microbial resistance and minimize microbial-related endodontic treatment failures.AIM. The aim of this study is to investigate the qualitative impact of standard irrigation solutions and a polyhexanide-based composition on the collagen matrix of dentin.MATERIALS AND METHODS. The study investigated the effect of nine solutions on the dentin of the tooth root. Twelve intact maxillary third molars extracted for orthodontic reasons were taken for the study. The teeth were fixed in a 10% neutral formalin solution for at least one day and were decalcinated. 5–10 μm thick sample sections were made and stained with picrosirius (Picrosirius Red, Biovitrum, Russia) before being examined under polarized light. The histological slice was analyzed using a Carl Zeiss (Germany) software and hardware complex based on ZEN v3.0 and light microscope Axioimager M.1. All microphotos were loaded into the Fiji distribution program of ImageJ for microscopy pixel classification.RESULTS. Upon analysis of the diagrams, it appears that there is a lower amount and density of stained dentin collagen array after exposure to sodium hypochlorite solutions of 3% and 1.5%, as well as polyhexanide.CONCLUSIONS. The data obtained from the histogram analyzer of samples stained with picrosirius suggests that polyhexanide may have a qualitative effect on the organic structure of hard tissues, particularly collagen.

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Section: Discussionmentioning

confidence: 99%

The effect of the irrigation solutions on dentin organic components: Pilot study

Khabadze,

Generalova,

Taptun

et al. 2024

Èndodontiâ today

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“…The exact target composition of the Herovici stain remains undefined. Herovici's stain has been used to report and relatively quantify Collagen Types I and III as well as immature and mature collagen [108–113], with controversy regarding the type of collagen that is stained.…”

Section: Spatial Analysis Of the Extracellular Microenvironmentmentioning

confidence: 99%

Molecular analysis of the extracellular microenvironment: from form to function

Macdonald,

Mehta,

Drake

et al. 2024

FEBS Letters

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The extracellular matrix (ECM) proteome represents an important component of the tissue microenvironment that controls chemical flux and induces cell signaling through encoded structure. The analysis of the ECM represents an analytical challenge through high levels of post‐translational modifications, protease‐resistant structures, and crosslinked, insoluble proteins. This review provides a comprehensive overview of the analytical challenges involved in addressing the complexities of spatially profiling the extracellular matrix proteome. A synopsis of the process of synthesizing the ECM structure, detailing inherent chemical complexity, is included to present the scope of the analytical challenge. Current chromatographic and spatial techniques addressing these challenges are detailed. Capabilities for multimodal multiplexing with cellular populations are discussed with a perspective on developing a holistic view of disease processes that includes both the cellular and extracellular microenvironment.

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“…It consists of 70% hydroxyapatite, 20% organic matrix and 10% water [ 5 ]. The main part of the organic matrix is formed by collagen type I [ 3 ], collagen type III can also be found [ 6 ]. It is permeated by dentinal tubules, which contain the cellular extensions of odontoblasts located in the pulp.…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that changes in the collagen structure of dentin can be detected [ 20 ]. A recent study focussed on the investigation of the alignment of collagen fibrils in dentin [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Visualization of carious lesions with polarized and depolarized light microscopy

Grundmann,

Golde,

Steuer

et al. 2024

Biomed. Opt. Express

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Polarized light microscopy (PLM) is an established method in dental histology for investigating the ultrastructure and carious process of teeth. This study introduces a novel approach for measuring the degree of polarization (DOP) in a modified PLM setup and uses the DOP to assess the changes of the optical properties of enamel and dentin due to caries. The validation is provided by a comparison with complementary imaging methods, i.e. standard PLM and µCT. The results show that demineralization is reliably displayed by the DOP in accordance with the common imaging methods, and that this quantitative analysis of depolarization allows the characterization of the different pathohistological zones of caries.

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Dynamic dentin: A quantitative microscopic assessment of age and spatial changes to matrix architecture, peritubular dentin, and collagens types I and III

Cited by 6 publications

References 54 publications

The effect of the irrigation solutions on dentin organic components: Pilot study

The effect of the irrigation solutions on dentin organic components: Pilot study

Molecular analysis of the extracellular microenvironment: from form to function

Visualization of carious lesions with polarized and depolarized light microscopy

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