Dynamic Diatom-Bacteria Consortia in Synthetic Plankton Communities

Deng, Yun; Mauri, M.; Vallet, Marine; Staudinger, Mona; Allen, Rosalind J.; Pohnert, Georg

doi:10.1128/aem.01619-22

Cited by 18 publications

(13 citation statements)

References 59 publications

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“…They represent two most abundant genetic clades in the marine environment which differ in their utilization and metabolization of DMSP (Scheme 2). 28 The strain CS4 contains the proteins for both the DMSP demethylation (DmdA, B, C, and D) and cleavage pathway (DddW and DddP) (Scheme 2 and Fig. S18, ESI †).…”

Section: Resultsmentioning

confidence: 99%

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Dimethylsulfoniopropionate decorated cryogels as synthetic spatially structured habitats of marine bacterial communities

et al. 2023

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Section: Resultsmentioning

confidence: 99%

“…They represent two most abundant genetic clades in the marine environment which differ in their utilization and metabolization of DMSP (Scheme 2). 28…”

Section: Resultsmentioning

confidence: 99%

Dimethylsulfoniopropionate decorated cryogels as synthetic spatially structured habitats of marine bacterial communities

et al. 2023

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“…To determine whether any of our isolates had prior reports of being algicidal, we conducted a literature review. This revealed that antagonistic activity against algae has been reported previously in eight of the genera of the WEC isolates, including Ponticoccus (Chi et al ., 2017), Thalassospira (Lu et al ., 2016), Halomonas (Zheng et al ., 2018), Marinobacter (Zheng et al ., 2018; Deng et al ., 2022), Vibrio (Li et al ., 2014), Pseudomonas (Aiyar et al ., 2017; Kim et al ., 2018), Alteromonas (Wang et al ., 2016; Shi et al ., 2018), and Maribacter (Wang et al ., 2016; Fig. 2c).…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, we observed a robust growth inhibitory effect of M. adhaerens against Chaetoceros sp. PLY617, despite this bacterium more commonly being considered a diatom commensal (Gärdes et al ., 2012; albeit a subtle inhibitory effect on Coscinodiscus radiatus growth by this bacterium was recently reported; Deng et al ., 2022). Indeed, this effect was only observed when cells were first grown on medium made up of autoclaved diatoms.…”

Section: Discussionmentioning

confidence: 99%

Cryptic bacterial pathogens of diatoms peak during senescence of a winter diatom bloom

Branscombe,

Harrison,

Choong

et al. 2023

New Phytologist

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Summary Diatoms are globally abundant microalgae that form extensive blooms in aquatic ecosystems. Certain bacteria behave antagonistically towards diatoms, killing or inhibiting their growth. Despite their crucial implications to diatom bloom and population health, knowledge of diatom antagonists in the environment is fundamentally lacking. We report systematic characterisation of the diversity and seasonal dynamics of bacterial antagonists of diatoms via plaque assay sampling in the Western English Channel, where diatoms frequently bloom. Unexpectedly, peaks in detection did not occur during characteristic spring diatom blooms, but coincided with a winter bloom of Coscinodiscus, suggesting that these bacteria likely influence distinct diatom host populations. We isolated multiple bacterial antagonists, spanning 4 classes and 10 bacterial orders. Notably, a diatom attaching Roseobacter Ponticoccus alexandrii was isolated multiple times, indicative of a persistent environmental presence. Moreover, many isolates had no prior reports of antagonistic activity towards diatoms. We verified diatom growth inhibitory effects of eight isolates. In all cases tested, these effects were activated by pre‐exposure to diatom organic matter. Discovery of widespread ‘cryptic’ antagonistic activity indicates that bacterial pathogenicity towards diatoms is more prevalent than previously recognised. Finally, examination of the global biogeography of WEC antagonists revealed co‐occurrence patterns with diatom host populations in marine waters globally.

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“…As with land plants and other host–microbial systems, microalgal activity, productivity, and stability are closely tied to surrounding microbial communities (Seymour et al ., 2017). However, a predictive understanding of microbial community interactions with algae is still limited (Astacio et al ., 2021; Deng et al ., 2022; Prabhakara & Kuehn, 2022).…”

Section: Introductionmentioning

confidence: 99%

DynamicPhaeodactylum tricornutumexometabolites shape surrounding bacterial communities

et al. 2023

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Summary Roles of different ecological classes of algal exometabolites in regulating microbial community composition are not well understood. Here, we identify exometabolites from the model diatom Phaeodactylum tricornutum and demonstrate their potential to influence bacterial abundances. We profiled exometabolites across a time course of axenic algal growth using liquid chromatography–tandem mass spectrometry. We then investigated growth of 12 bacterial isolates on individual‐identified exometabolites. Lastly, we compared responses of a P. tricornutum‐adapted enrichment community to additions of two contrasting metabolites: selective growth substrate 4‐hydroxybenzoic acid and putative signaling/facilitator molecule lumichrome. We identified 50 P. tricornutum metabolites and found distinct temporal accumulation patterns. Two exometabolites (of 12 tested) supported growth of distinct subsets of bacterial isolates. While algal exudates and algal presence drove similar changes in community composition compared with controls, exogenous 4‐hydroxybenzoic acid addition promoted increased abundances of taxa that utilized it in isolation, and also revealed the importance of factors relating to algal presence in regulating community composition. This work demonstrates that secretion of selective bacterial growth substrates represents one mechanism by which algal exometabolites can influence bacterial community composition and illustrates how the algal exometabolome has the potential to modulate bacterial communities as a function of algal growth.

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Dynamic Diatom-Bacteria Consortia in Synthetic Plankton Communities

Abstract: The marine food web is fueled by phototrophic phytoplankton. These algae are central primary producers responsible for the fixation of ca. 40% of the global CO 2 .

Cited by 18 publications

References 59 publications

Dimethylsulfoniopropionate decorated cryogels as synthetic spatially structured habitats of marine bacterial communities

Dimethylsulfoniopropionate decorated cryogels as synthetic spatially structured habitats of marine bacterial communities

Cryptic bacterial pathogens of diatoms peak during senescence of a winter diatom bloom

DynamicPhaeodactylum tricornutumexometabolites shape surrounding bacterial communities

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