Dynamic expression of Tbx2 subfamily genes in development of the mouse reproductive system

Douglas, Nataki C.; Heng, Kathleen; Sauer, Mark V.; Papaioannou, Virginia E.

doi:10.1002/dvdy.23710

Cited by 24 publications

(22 citation statements)

References 50 publications

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“…Like many T-box genes, Tbx3 is expressed in a highly dynamic pattern throughout development. Notably, it is present in the developing limbs, mammary gland, craniofacial region, heart, kidney, Wolffian duct epithelium, and mesenchyme around both Wolffian and Müllerian ducts, pituitary gland, dorsal root ganglia, yolk sac, and lungs (Chapman et al, 1996;Douglas et al, 2012). Expression is found in the embryonic precursors of many -if not all -of the tissues affected in UMS, as well as in a number of other tissues not known to be affected in patients with UMS.…”

Section: Tbx3 and The Ulnar Mammary Syndromementioning

confidence: 99%

T-Box Genes and Developmental Anomalies

Douglas

Washkowitz

Naiche

et al. 2015

Principles of Developmental Genetics

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Section: Tbx3 and The Ulnar Mammary Syndromementioning

confidence: 99%

T-Box Genes and Developmental Anomalies

Douglas

Washkowitz

Naiche

et al. 2015

Principles of Developmental Genetics

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“…Antisense probes for Tbx2, Tbx4, Tbx5, and Pou5f1 were used as previously described [10], and sections were counterstained with Nuclear Fast Red (Sigma) unless otherwise indicated. ISH with sense RNA controls showed no background staining.…”

Section: In Situ Hybridization Immunofluorescence and Histologymentioning

confidence: 99%

“…Tbx4 expression in the bipotential embryonic gonad is first detected at E11.5 [10]. To determine if Tbx4 is expressed in embryonic germ cells or in the surrounding tissue, we analyzed Tbx4 and Pou5f1 expression following busulfan treatment of pregnant females.…”

Section: Expression Of Tbx4 In Germ Cellsmentioning

confidence: 99%

“…We recently described the expression of the TBX2 subfamily of T-box transcription factor genes, which includes Tbx4, during development of the mammalian reproductive system [10]. The T-box family of transcription factors, defined by a conserved DNA-binding domain, the T-box, plays a critical role in the determination of cell fate decisions during organogenesis and in the differentiation of many organ systems [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…The similarities between mice and human phenotypes underlie the importance of T-box genes in mammalian development and strongly suggest conservation of function among different species. Tbx4 is the only member of the TBX2 subfamily expressed in germ cells [10]. Despite this expression, the function of Tbx4 in germ cell development in mice or humans has not as yet been described.…”

Section: Introductionmentioning

confidence: 99%

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Investigating the Role of Tbx4 in the Female Germline in Mice1

Douglas

Arora

Chen

et al. 2013

Biology of Reproduction

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Normal development of germ cells is essential for fertility and mammalian reproduction. Although abnormal development of oocytes or follicles may lead to primary ovarian insufficiency (POI), a disorder that causes infertility in 1% of women less than 40 yr of age, the genes and signaling pathways activated in POI are not as yet fully elucidated. Tbx4, a member of the T-box family of transcription factors, is expressed in embryonic germ cells and postnatal oocytes at all stages of folliculogenesis. To investigate the requirement for Tbx4 in the germline, we analyzed germ cell development in the absence of Tbx4. We show that primordial germ cells (PGCs) are reduced in Tbx4 homozygous null (Tbx4(-/-)) embryos at Embryonic Day (E) 10.0. Tbx4(-/-) embryos die by E10.5; to study later time points in vitro, a tamoxifen-inducible estrogen receptor Cre recombinase was used to delete Tbx4 conditional mutant alleles. In addition, Gdf9cre and Zp3cre, two oocyte-specific Cre recombinases, were used to delete Tbx4 from postnatal primordial and primary follicles, respectively. We show that in vitro differentiation of the gonad into morphologically distinct testes and ovaries occurs normally starting at E11.5 when Tbx4 is deleted. In Gdf9cre; Tbx4(fl/-) and Zp3cre; Tbx4(fl/-) adult females, primordial, primary, secondary, and antral follicles form, ovulation occurs, corpus luteum formation is normal, and the mice are fertile without any evidence of diminished ovarian reserve. Although postnatal deletion of Tbx4 in oocytes does not obviously impair fertility, it is possible that the reduction in PGCs observed in Tbx4 homozygous null mutant embryos could affect long-term fertility in adults.

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T‐Box Genes: Developmental Functions in Mammals

Papaioannou

2017

Encyclopedia of Life Sciences

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Transcription factors encoded by the T‐box gene family control key functions throughout development in all metazoans. The DNA (deoxyribonucleic acid)‐binding domain is highly conserved. There are 17 genes in mouse and human, all with diverse functional roles from early embryogenesis through organogenesis and tissue homeostasis. The same gene may function in different tissues and at different stages such that mutation phenotypes can be complex. Most T‐box mutations display heterozygous defects, indicating dose sensitivity. Several T‐box genes affect pluripotency and differentiation in the pre‐ and early postimplantation embryo; limb outgrowth and differentiation is a major developmental area affected by T‐box genes as is the heart, nervous system, immune system and many other organs and tissues. T‐box gene loss‐of‐function mutations in humans cause major developmental syndromes such as ulnar‐mammary and Holt–Oram syndrome, and other abnormalities are increasingly being attributed to alteration in T‐box gene function, including the association with many types of cancer. Key Concepts T‐box genes code for transcription factors with diverse roles in development through regulation of a variety of downstream target genes. They comprise an ancient gene family present in all metazoans as well as nonmetazoan lineages, defined by a conserved DNA‐binding motif. In vertebrates, T‐box genes play diverse and key roles in specification, differentiation and development of most organ systems. Mutations in T‐box genes result in complex birth defects and/or neonatal lethality and usually show effects in heterozygotes and homozygotes. Multiple tissues may be affected by the same T‐box gene, and multiple T‐box genes may affect the same tissue at the same or different times in development. Mutation or misregulation of T‐box genes is associated with a large variety of human cancers. Little information is available about the role of T‐box genes in adult organisms.

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Dynamic expression of Tbx2 subfamily genes in development of the mouse reproductive system

Cited by 24 publications

References 50 publications

T-Box Genes and Developmental Anomalies

T-Box Genes and Developmental Anomalies

Investigating the Role of Tbx4 in the Female Germline in Mice1

T‐Box Genes: Developmental Functions in Mammals

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