Dynamic gastric digestion of a commercial whey protein concentrate†

Miralles, Beatriz; Barrio, Roberto Del; Cueva, Carolina; Recio, Isidra; Amigo, Lourdes

doi:10.1002/jsfa.8668

Cited by 43 publications

(13 citation statements)

References 33 publications

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“…On the other hand, some protein fractions were highly resistant to gastrointestinal digestion (e.g., ∼70 kDa in NOPH, ∼15, 20 and 35-40 kDa in Arthospira platensis protein hydrolysates (APPH)). Miralles et al 25 found that serum albumin and ⊍-lactalbumin could not be detected at 30 and 60 min, while ⊎-lactoglobulin was recognized at 120 min during gastric digestion, which is similar to our results. The difference in hydrolysis susceptibility of four microalgae proteins may be associated with their various structure, spatial conformation, and the composition of amino acids.…”

Section: Protein Degradation In Vitro Gastrointestinal Digestionsupporting

confidence: 92%

Osteogenic activities of four calcium‐chelating microalgae peptides

Chen

Jin

2022

J Sci Food Agric

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BACKGROUND: Adequate calcium intake is necessary to prevent osteoporosis, which poses significant public health challenges. The natural bioactive peptide calcium chelates have been regarded as superior calcium supplements. Microalgae peptides are regarded as potential candidates for protection from bone loss in osteoporosis. This study aimed to prepare microalgae calcium-chelating peptides from four microalgae proteins and assess their osteogenic activities in osteoporosis-like zebrafish. RESULTS: After in vitro gastrointestinal digestion, 4.42% Chlorella pyrenoidosa protein, 2.74% Nannochloropsis oceanica protein, 6.07% Arthospira platensis protein and 10.47% Dunaliella salina protein were retained. The calcium-chelating capacities of four microalgae protein hydrolysates (MPHs) ranged from 14.10 ± 7.16% to 34.11 ± 9.34%. CaCl 2 addition increased the maximum absorption peaks, absorption intensities and particle sizes of MPHs. Calcium-chelating MPHs showed stronger osteogenic activities than MPHs in the osteoporosis-like zebrafish model, with significantly increased mineralized tissue area and integrated optical density.CONCLUSION: Microalgae proteins have favorable digestibilities. Among the four MPHs, Nannochloropsis oceanica protein hydrolysates showed the highest calcium-chelating capacity, which might be due to its high degree of hydrolysis after in vitro digestion and high content of Ser, Tyr, Thr, Asp and Glu. The absorption intensities and particle sizes of MPHs both increased after calcium addition. MPH treatment could reverse dexamethasone-induced osteoporosis of zebrafish, and MPHs-Ca chelates showed higher osteogenic activities in osteoporosis-like phenotype zebrafish.

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Section: Protein Degradation In Vitro Gastrointestinal Digestionsupporting

confidence: 92%

Osteogenic activities of four calcium‐chelating microalgae peptides

Chen

Jin

2022

J Sci Food Agric

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“…Generally, dynamic models to study dairy protein digestion and the bioaccessibility of AA are capable of mimicking the gastric digestion of milk proteins with a high predictive power (Miralles et al, 2018b). However, to date, the lack of standardization and the high cost of the dynamic models limits their widespread application.…”

Section: Dynamic Models To Study Dairy Bioactivesmentioning

confidence: 99%

Invited review: Dairy proteins and bioactive peptides: Modeling digestion and the intestinal barrier

Giromini

Cheli

Rebucci

et al. 2019

Journal of Dairy Science

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Dairy products are one of the most important sources of biologically active proteins and peptides. The healthpromoting functions of these peptides are related to their primary structure, which depends on the parent protein composition. A crucial issue in this field is the demonstration of a cause-effect relationship from the ingested protein form to the bioactive form in vivo. Intervention studies represent the gold standard in nutritional research; however, attention has increasingly been focused on the development of sophisticated in vitro models of digestion to elucidate the mechanism of action of dairy nutrients in a mechanistic way and significantly reduce the number of in vivo trials. On the other hand, the epithelial intestinal barrier is the first gate that actively interacts with digestion metabolites, making the intestinal cells the first target tissue of dairy nutrients and respective metabolites. An evolution of the in vitro digestion approach in the study of dairy proteins and derived bioactive compounds is the setup of combined in vitro digestion and cell culture models taking into consideration the endpoint to measure the target organism (e.g., animal, human) and the key concepts of bioaccessibility, bioavailability, and bioactivity. This review discusses the relevance and challenges of modeling digestion and the intestinal barrier, focusing on the implications for the modeling of dairy protein digestion for bioactivity evaluation.

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“…The authors selected the parameters of process simulation based on literature data to mimic in vivo conditions (Bellmann, Lelieveld, Gorissen, Minekus, & Havenaar, 2016) and preliminary experiments. 15,22,24 At the gastric level, the peristalsis frequency was set to 10 s −1 . The feeding volume of 80 mL of each GNMF was used in addition to a pH curve descending from the initial feeding pH to pH = 2 (fasting conditions) during the gastric digestion stage.…”

Section: Methodsmentioning

confidence: 99%