Whereas other biometric characteristics, such as the face, are readily available for an eventual attacker through social media or easy to capture with a conventional smartphone, vein patterns can only be acquired with dedicated sensors. This fact makes them relevant not only for recognition purposes but especially for Presentation Attack Detection (PAD), for instance, in combination with fingerprint recognition. In this chapter, we make use of this combination and present a finger vein-based PAD algorithm to detect presentation attacks targeting fingerprint recognition. The experiments are carried out on a newly collected database, comprising 32 species of Presentation Attack Instruments ranging from printed artefacts to more sophisticated fingerprint overlays. The results show that our method preserves a convenient usage while detecting around 90% of the attacks. However, thin and transparent fingerprint overlays remain very challenging.