Dynamic NanoSIMS ion imaging of unicellular freshwater algae exposed to copper

Slaveykova, Vera I.; Guignard, Cédric; Eybe, Tanja; Migeon, H.-N.; Hoffmann, Lucien

doi:10.1007/s00216-008-2486-x

Cited by 53 publications

(51 citation statements)

References 28 publications

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“…With increasing time increments, the ratio {Cu} ads /{Cu} int was significantly reduced and internalized Cu dominated the uptake. The above observations were in agreement with secondary 63 Cu -ion distribution in the individual C. kesslerii cells washed with EDTA (27), demonstrating that most of the Cu is in the Pand protein-containing compartments of the cell and that only a negligible amount of metal is at the cell periphery. This also independently confirms the efficiency of the EDTA extraction procedure (27).…”

Section: Resultssupporting

confidence: 84%

Effect of Humic Acid on Cd(II), Cu(II), and Pb(II) Uptake by Freshwater Algae: Kinetic and Cell Wall Speciation Considerations

Lamelas

Pinheiro

Slaveykova

2009

Environ. Sci. Technol.

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The present study examines the effect of humic acid on the uptake kinetics of Cd(II), Cu(II), and Pb(II) by the freshwater alga Chlorella kesslerii. The results demonstrated that the relative proportion of Pb in the cell wall layer is greater than that of the internalized Pb, while internalized Cd and Cu were comparable or greater than the adsorbed metal concentration. In the presence of 10 mg L -1 humic acid (HA), Cd and Cu uptake kinetics were consistent with that predicted by measured free metal concentrations. For Pb, the uptake flux and amount of lead bound to internalization and adsorption sites were an order of magnitude higher than those found at the same free lead ion concentrations in the presence of citric acid. Chemodynamic modeling suggested that the enhancement of the Pb uptake flux in the presence of HA originates from an increasing amount of Pb bound to the internalization sites through a ternary complex formation between lead-humic acid complex and internalization sites. Cell wall speciation calculations indicated that the lead-humic acid complex is the predominant species in the cell wall layer, while for Cu(II) and Cd(II) metal bound to the internalization (Cu) and adsorption (Cd) sites significantly dominated over the M-HA complex. The findings of the work show the relevance of the cell wall layer concentration and speciation and its key role in defining the local equilibrium conditions between metal and internalizations sites. The results of the present kinetic study have important consequences for improvement of the mechanistic understanding of the role of dissolved organic matter in metal uptake in phytoplankton and biogeochemical cycling of metals in the surface waters.

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Section: Resultssupporting

confidence: 84%

Effect of Humic Acid on Cd(II), Cu(II), and Pb(II) Uptake by Freshwater Algae: Kinetic and Cell Wall Speciation Considerations

Lamelas

Pinheiro

Slaveykova

2009

Environ. Sci. Technol.

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“…MSI has been employed to analyze many classes of samples, examples include everything from unicellular algae [36], cell cultures [37], plant material [23,38], surgically dissected organs and tissue biopsies [39], to whole animals [40][41][42]. As already alluded to, for all samples it is vital that rapid and consistent collection protocols are used to prevent rapid delocalization or degradation of target compounds, such as have been reported in neuropeptidomic abundance studies in post-mortem brain [35,43].…”

Section: Collectionmentioning

confidence: 98%

“…Peptide analysis of neuronal cell cultures have been performed, employing the stretch method described earlier [37]. SIMS has been used to image copper ion distributions in microalgal cells following fixation [36], while SIMS and MALDI analyses have been combined to image in situ the localization and quantification of surfactins in a Bacillus subtilis swarming community [139]. Finally, an interesting alternative to direct sample analysis has been used by researchers using DESI to image the distribution of leaf chlorophyll degradation products using imprinting techniques [38] reminiscent of early MALDI MSI blotting experiments [1] and sample contact transfer to silicon surfaces for DIOS analysis [75].…”

Section: Novel Applications Of Msi and Associated Sample Preparationmentioning

confidence: 99%

Sample preparation for mass spectrometry imaging: Small mistakes can lead to big consequences

Goodwin

2012

Journal of Proteomics

271

249

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“…NanoSIMS can also measure the cellular distribution of other nutrients such as copper or iron (31,162) and can reveal the ability of microbes to take up specific, labeled organic molecules (e.g., algal exudates) (124). Applying this technique to known diatombacterium interactions will provide valuable information on the flux of nutrients from one organism to another and the net production by these organisms in the ecosystem.…”

Section: Synergistic Interactionsmentioning

confidence: 99%

Interactions between Diatoms and Bacteria

Amin

Parker

Armbrust

2012

Microbiol Mol Biol Rev

837

741

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SUMMARY Diatoms and bacteria have cooccurred in common habitats for hundreds of millions of years, thus fostering specific associations and interactions with global biogeochemical consequences. Diatoms are responsible for one-fifth of the photosynthesis on Earth, while bacteria remineralize a large portion of this fixed carbon in the oceans. Through their coexistence, diatoms and bacteria cycle nutrients between oxidized and reduced states, impacting bioavailability and ultimately feeding higher trophic levels. Here we present an overview of how diatoms and bacteria interact and the implications of these interactions. We emphasize that heterotrophic bacteria in the oceans that are consistently associated with diatoms are confined to two phyla. These consistent bacterial associations result from encounter mechanisms that occur within a microscale environment surrounding a diatom cell. We review signaling mechanisms that occur in this microenvironment to pave the way for specific interactions. Finally, we discuss known interactions between diatoms and bacteria and exciting new directions and research opportunities in this field. Throughout the review, we emphasize new technological advances that will help in the discovery of new interactions. Deciphering the languages of diatoms and bacteria and how they interact will inform our understanding of the role these organisms have in shaping the ocean and how these interactions may change in future oceans.

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Dynamic NanoSIMS ion imaging of unicellular freshwater algae exposed to copper

Cited by 53 publications

References 28 publications

Effect of Humic Acid on Cd(II), Cu(II), and Pb(II) Uptake by Freshwater Algae: Kinetic and Cell Wall Speciation Considerations

Effect of Humic Acid on Cd(II), Cu(II), and Pb(II) Uptake by Freshwater Algae: Kinetic and Cell Wall Speciation Considerations

Sample preparation for mass spectrometry imaging: Small mistakes can lead to big consequences

Interactions between Diatoms and Bacteria

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