Dynamic response of plant genome to ultraviolet radiation and other genotoxic stresses

Molinier, Jean; Oakeley, Edward J.; Niederhauser, Olivier; Kovalchuk, Igor; Höhn, Barbara

doi:10.1016/j.mrfmmm.2004.09.016

Cited by 71 publications

(66 citation statements)

References 44 publications

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“…However, information on the transcriptional regulation of DSB repair genes is rather limited. In plants, Ataxia Telangiectasia Mutated Kinase regulates the transcriptional DNA damage response, which is highly specific to DSB-inducing genotoxins rather than a general response to DNA damage (Molinier et al, 2005). In our study, transcriptional up-regulation of the NHEJ-related genes AtKU80, AtXRCC4, and AtLig4 in addition to AtPoll in wild-type Arabidopsis seedlings after high NaCl and MMC treatments (Fig.…”

Section: Discussionmentioning

confidence: 52%

Involvement of AtPolλ in the Repair of High Salt- and DNA Cross-Linking Agent-Induced Double Strand Breaks in Arabidopsis

et al. 2013

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DNA polymerase l (Pol l) is the sole member of family X DNA polymerase in plants and plays a crucial role in nuclear DNA damage repair. Here, we report the transcriptional up-regulation of Arabidopsis (Arabidopsis thaliana) AtPoll in response to abiotic and genotoxic stress, including salinity and the DNA cross-linking agent mitomycin C (MMC). The increased sensitivity of atpoll knockout mutants toward high salinity and MMC treatments, with higher levels of accumulation of double strand breaks (DSBs) than wild-type plants and delayed repair of DSBs, has suggested the requirement of Pol l in DSB repair in plants.AtPoll overexpression moderately complemented the deficiency of DSB repair capacity in atpoll mutants. Transcriptional upregulation of major nonhomologous end joining (NHEJ) pathway genes KU80, X-RAY CROSS COMPLEMENTATION PROTEIN4 (XRCC4), and DNA Ligase4 (Lig4) along with AtPoll in Arabidopsis seedlings, and the increased sensitivity of atpoll-2/atxrcc4 and atpoll-2/atlig4 double mutants toward high salinity and MMC treatments, indicated the involvement of NHEJ-mediated repair of salinity-and MMC-induced DSBs. The suppressed expression of NHEJ genes in atpoll mutants suggested complex transcriptional regulation of NHEJ genes. Pol l interacted directly with XRCC4 and Lig4 via its N-terminal breast cancer-associated C terminus (BRCT) domain in a yeast two-hybrid system, while increased sensitivity of BRCT-deficient Pol l-expressing transgenic atpoll-2 mutants toward genotoxins indicated the importance of the BRCT domain of AtPoll in mediating the interactions for processing DSBs. Our findings provide evidence for the direct involvement of DNA Pol l in the repair of DSBs in a plant genome.

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Section: Discussionmentioning

confidence: 52%

Involvement of AtPolλ in the Repair of High Salt- and DNA Cross-Linking Agent-Induced Double Strand Breaks in Arabidopsis

et al. 2013

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“…However, Boyko et al (2010a) were able to detect such an effect, but only in the first generation of progeny. Similarly, in contrast with Molinier et al (2005) and Yao et al (2011), who found a seven-or threefold increase in recombination frequency, respectively, as a result of UV-C irradiation in Arabidopsis, Van der Auwera et al (2008) did not find a significant effect of UV-C in their SHR lines containing GUS recombination reporter (Van der Auwera et al, 2008). Furthermore, in contrast with earlier results (Kovalchuk et al, 2001b;Boyko et al, 2005), Van der Auwera et al (2008) reported that the presence of heavy metals, such as lead or cadmium ions, a heat shock of 50˚C, or growth at elevated temperatures and increased daylength had no measurable effect on the mutation or recombination frequencies .…”

Section: Conflicting Results From Shr Reporter Linesmentioning

confidence: 92%

“…Analysis of several independent reporter lines carrying these marker genes indicated that the average somatic recombination frequency detected with the LUC transgene was ;10-fold higher (10 25 ) in Arabidopsis thaliana and tobacco (Nicotiana tabacum) plants compared with GUS recombination frequencies (Ilnytskyy et al, 2004). Using such GUS-or LUC-based SHR reporter lines, it was shown that abiotic and especially biotic stresses lead to enhanced somatic recombination (Kovalchuk et al, 1998(Kovalchuk et al, , 2001a(Kovalchuk et al, , 2001b(Kovalchuk et al, , 2003Lucht et al, 2002;Filkowski et al, 2004;Ilnytskyy et al, 2004;Molinier et al, 2004Molinier et al, , 2005Molinier et al, , 2006Schuermann et al, 2005). Using such a system, it was also discovered that plants can remember a stress perceived in one generation for several following generations.…”

Section: Somatic Homologous Recombination Reporter Lines: Lack Of Sufmentioning

confidence: 99%

Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

et al. 2012

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A widely used approach for assessing genome instability in plants makes use of somatic homologous recombination (SHR) reporter lines. Here, we review the published characteristics and uses of SHR lines. We found a lack of detailed information on these lines and a lack of sufficient evidence that they report only homologous recombination. We postulate that instead of SHR, these lines might be reporting a number of alternative stress-induced stochastic events known to occur at transcriptional, posttranscriptional, and posttranslational levels. We conclude that the reliability and usefulness of the somatic homologous recombination reporter lines requires revision. Thus, more detailed information about these reporter lines is needed before they can be used with confidence to measure genome instability, including the complete sequences of SHR constructs, the genomic location of reporter genes and, importantly, molecular evidence that reconstituted gene expression in these lines is indeed a result of somatic recombination.

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“…Many stresses were shown to alter the frequency of somatic homologous recombination (HRF), including gamma-and Xrays, exposures to UV-C and UV-B, treatments with chemicals causing oxidative stress, herbicides as well as changes in temperature and water regimes and infection with pathogens (Besplug et al 2004;Boyko et al 2005Boyko et al , 2010aFilkowski et al 2004;Kathiria et al 2010;Kovalchuk et al 2000;Lucht et al 2002;Molinier et al 2005;Pecinka et al 2009;Ries et al 2000;Yao and Kovalchuk 2011). The influence of stress on the frequency of homologous recombination cannot be underestimated since HR is able to rearrange the plant genome substantially, thus leading to loss of heterozygocity.…”

Section: Introductionmentioning

confidence: 99%

Changes in homologous recombination frequency in Arabidopsis thaliana plants exposed to stress depend on time of exposure during development and on duration of stress exposure

Rahavi

Kovalchuk

2013

Physiol Mol Biol Plants

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In the past, we showed that exposure to abiotic and biotic stresses changes the homologous recombination frequency (HRF) in somatic tissue and in the progeny. In current work we planned to answer the following question: do stress intensity/duration and time during exposure influence changes in somatic HRF and transgenerational changes in HRF? Here, we tested the effects of exposure to UV-C, cold and heat on HRF at 7, 14, 21 and 28 days post germination (dpg). We found that exposure at 14 and 21 dpg resulted in a higher increase in HRF as compared to exposure at 7 dpg; longer exposure to UV-C resulted in a higher frequency of HR, whereas prolonged exposure to cold or heat, especially at later developmental stages, had almost no effect on somatic HRF. Exposure at 7 dpg had a positive effect on somatic growth of plants; plants exposed to stress at this age had larger leaves. The analysis of HRF in the progeny showed that the progeny of plants exposed to stress at 7 dpg had an increase in somatic HRF and showed larger sizes of recombination spots on leaves. The progeny of plants exposed to UV-C at 7 dpg and the progeny of plants exposed to cold or heat at 28 dpg had larger leaves as compared to control plants. To summarize, our experiments showed that changes in somatic and transgenerational HRF depend on the type of stress plants are exposed to, time of exposure during development and the duration of exposure.

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Dynamic response of plant genome to ultraviolet radiation and other genotoxic stresses

Cited by 71 publications

References 44 publications

Involvement of AtPolλ in the Repair of High Salt- and DNA Cross-Linking Agent-Induced Double Strand Breaks in Arabidopsis

Involvement of AtPolλ in the Repair of High Salt- and DNA Cross-Linking Agent-Induced Double Strand Breaks in Arabidopsis

Reevaluation of the Reliability and Usefulness of the Somatic Homologous Recombination Reporter Lines

Changes in homologous recombination frequency in Arabidopsis thaliana plants exposed to stress depend on time of exposure during development and on duration of stress exposure

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