Dynamic stiffening of poly(ethylene glycol)-based hydrogels to direct valvular interstitial cell phenotype in a three-dimensional environment

Mabry, Kelly M.; Lawrence, Rosa L.; Anseth, Kristi S.

doi:10.1016/j.biomaterials.2015.01.047

Cited by 192 publications

(187 citation statements)

References 41 publications

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“…13,22 One of the most commonly used synthetic polymers to investigate the effects of mechanical stimuli on cellular behavior is poly(ethylene glycol) (PEG), which provides precise control of material stiffness. 23 PEG is an interesting hydrogel material with its good water solubility, biocompatibility, nonimmunogenicity, and resistance to protein adsorption. 24 However, PEG cannot provide cell attachment and induce further cell−material interactions due to its proteinrepellent property.…”

Section: ■ Introductionmentioning

confidence: 99%

Self-Assembled Peptide Amphiphile Nanofibers and PEG Composite Hydrogels as Tunable ECM Mimetic Microenvironment

Göktas

Çınar

Orujalipoor³

et al. 2015

Biomacromolecules

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Natural extracellular matrix (ECM) consists of complex signals interacting with each other to organize cellular behavior and responses. This sophisticated microenvironment can be mimicked by advanced materials presenting essential biochemical and physical properties in a synergistic manner. In this work, we developed a facile fabrication method for a novel nanofibrous self-assembled peptide amphiphile (PA) and poly(ethylene glycol) (PEG) composite hydrogel system with independently tunable biochemical, mechanical, and physical cues without any chemical modification of polymer backbone or additional polymer processing techniques to create synthetic ECM analogues. This approach allows noninteracting modification of multiple niche properties (e.g., bioactive ligands, stiffness, porosity), since no covalent conjugation method was used to modify PEG monomers for incorporation of bioactivity and porosity. Combining the self-assembled PA nanofibers with a chemically cross-linked polymer network simply by facile mixing followed by photopolymerization resulted in the formation of porous bioactive hydrogel systems. The resulting porous network can be functionalized with desired bioactive signaling epitopes by simply altering the amino acid sequence of the self-assembling PA molecule. In addition, the mechanical properties of the composite system can be precisely controlled by changing the PEG concentration. Therefore, nanofibrous self-assembled PA/ PEG composite hydrogels reported in this work can provide new opportunities as versatile synthetic mimics of ECM with independently tunable biological and mechanical properties for tissue engineering and regenerative medicine applications. In addition, such systems could provide useful tools for investigation of how complex niche cues influence cellular behavior and tissue formation both in two-dimensional and three-dimensional platforms.

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Section: ■ Introductionmentioning

confidence: 99%

Self-Assembled Peptide Amphiphile Nanofibers and PEG Composite Hydrogels as Tunable ECM Mimetic Microenvironment

Göktas

Çınar

Orujalipoor³

et al. 2015

Biomacromolecules

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“…Moreover, signal transductions of cells were revealed to be completely different between 2D and 3D culture methods. 5,6 These findings strongly suggest that cells change their behaviors and functions by sensing the surrounding spatial environment.…”

Section: Introductionmentioning

confidence: 91%

Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture

et al. 2016

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“…Stiffening of cell-laden PEG hydrogels was achieved by diffusing 8-arm PEG derivatives into the base hydrogel to form the second crosslinking network. Responses of valvular interstitial cells encapsulated in 3D hydrogels to the mechanical change were evaluated to reveal different behaviors with those from 2D cell culture models [259]. Recently, dynamic hydrogels capable of in situ secondary crosslinking was obtained by using an ABA-triblock copolymer.…”

Section: Temporal Control Of Hydrogelmentioning

confidence: 99%

Spatially and temporally controlled hydrogels for tissue engineering

Leijten

Seo

Yue

et al. 2017

Materials Science and Engineering: R: Reports

159

129

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Summary Recent years have seen tremendous advances in the field of hydrogel-based biomaterials. One of the most prominent revolutions in this field has been the integration of elements or techniques that enable spatial and temporal control over hydrogels’ properties and functions. Here, we critically review the emerging progress of spatiotemporal control over biomaterial properties towards the development of functional engineered tissue constructs. Specifically, we will highlight the main advances in the spatial control of biomaterials, such as surface modification, microfabrication, photo-patterning, and three-dimensional (3D) bioprinting, as well as advances in the temporal control of biomaterials, such as controlled release of molecules, photocleaving of proteins, and controlled hydrogel degradation. We believe that the development and integration of these techniques will drive the engineering of next-generation engineered tissues.

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Dynamic stiffening of poly(ethylene glycol)-based hydrogels to direct valvular interstitial cell phenotype in a three-dimensional environment

Cited by 192 publications

References 41 publications

Self-Assembled Peptide Amphiphile Nanofibers and PEG Composite Hydrogels as Tunable ECM Mimetic Microenvironment

Self-Assembled Peptide Amphiphile Nanofibers and PEG Composite Hydrogels as Tunable ECM Mimetic Microenvironment

Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture

Spatially and temporally controlled hydrogels for tissue engineering

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