Dynamic <sup>31</sup>P MR spectroscopy of plantar flexion: Influence of ergometer design, magnetic field strength (3 and 7 T), and RF‐coil design

Šedivý, Petr; Kipfelsberger, Monika Christina; Dezortová, Monika; Krššák, Martin; Drobný, M; Chmelík, Marek; Rydlo, Jan; Trattnig, Siegfried; Hájek, Milan; Valkovič, Ladislav

doi:10.1118/1.4914448

Cited by 30 publications

(37 citation statements)

References 55 publications

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“…Calculated Q max values are broadly within the range of previous reports471516. With protocol A, no significant difference of Q max was found between the two muscles, although measurements using protocol B showed higher values of Q max in SOL.…”

Section: Discussionsupporting

confidence: 84%

Skeletal muscle ATP synthesis and cellular H+ handling measured by localized 31P-MRS during exercise and recovery

Fiedler

Schmid

Goluch

et al. 2016

Sci Rep

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31P magnetic resonance spectroscopy (MRS) is widely used for non-invasive investigation of muscle metabolism dynamics. This study aims to extend knowledge on parameters derived from these measurements in detail and comprehensiveness: proton (H+) efflux, buffer capacity and the contributions of glycolytic (L) and oxidative (Q) rates to ATP synthesis were calculated from the evolutions of phosphocreatine (PCr) and pH. Data are reported for two muscles in the human calf, for each subject and over a wide range of exercise intensities. 22 subjects performed plantar flexions in a 7T MR-scanner, leading to PCr changes ranging from barely noticeable to almost complete depletion, depending on exercise protocol and muscle studied by localized MRS. Cytosolic buffer capacity was quantified for the first time non-invasively and individually, as was proton efflux evolution in early recovery. Acidification started once PCr depletion reached 60–75%. Initial and end-exercise L correlated with end-exercise levels of PCr and approximately linear with pH. Q calculated directly from PCr and pH derivatives was plausible, requiring fewer assumptions than the commonly used ADP-model. In conclusion, the evolution of parameters describing cellular energy metabolism was measured over a wide range of exercise intensities, revealing a relatively complete picture of muscle metabolism.

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Section: Discussionsupporting

confidence: 84%

Skeletal muscle ATP synthesis and cellular H+ handling measured by localized 31P-MRS during exercise and recovery

Fiedler

Schmid

Goluch

et al. 2016

Sci Rep

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“…However, due to their costs and/or specific application demands, there are many more built-in-the-lab devices used worldwide [20], [104], [131], [132], [133], [134], [135], [136], [137], [138], [139]. Basic designs apply mechanical or pneumatic workload settings.…”

Section: P-mrs Of Skeletal Musclementioning

confidence: 99%

“…metabolism of which muscle group is to be investigated and what type of exercise is suitable for the task at hand. This is of particular importance when a multi-centric study is planned, and the results from dynamic experiments performed at different research sites are to be directly compared [20].…”

Section: P-mrs Of Skeletal Musclementioning

confidence: 99%

“…a broadband transmitter and a receiver, as well as RF-coils, is necessary. To compensate for the low sensitivity of MR systems to 31 P, surface coils that offer very high sensitivity in their close vicinity are often applied for organ-specific 31 P-MR investigations [17], [18], [19], [20]. These are commonly constructed as simple single loops; however, several complex coil-arrays that provide higher sensitivity and better volume coverage have been introduced recently [21], [22], [23], [24].…”

Section: Introductionmentioning

confidence: 99%

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In-vivo 31P-MRS of skeletal muscle and liver: A way for non-invasive assessment of their metabolism

Valkovič

Chmelík

Krššák

2017

Analytical Biochemistry

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In addition to direct assessment of high energy phosphorus containing metabolite content within tissues, phosphorus magnetic resonance spectroscopy (31P-MRS) provides options to measure phospholipid metabolites and cellular pH, as well as the kinetics of chemical reactions of energy metabolism in vivo. Even though the great potential of 31P-MR was recognized over 30 years ago, modern MR systems, as well as new, dedicated hardware and measurement techniques provide further opportunities for research of human biochemistry. This paper presents a methodological overview of the 31P-MR techniques that can be used for basic, physiological, or clinical research of human skeletal muscle and liver in vivo. Practical issues of 31P-MRS experiments and examples of potential applications are also provided. As signal localization is essential for liver 31P-MRS and is important for dynamic muscle examinations as well, typical localization strategies for 31P-MR are also described.

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“…Different types of ergometers have been developed over the years, which either use pneumatic cylinders [24–28], elastic bands [29, 30], or weighted pulleys to allow variable and adjustable resistance to exercise [30, 31]. Several commercially available ergometers offer different exercise modules, though these can be cost prohibitive for many research labs [32]. …”

Section: Introductionmentioning

confidence: 99%

A low-cost Mr compatible ergometer to assess post-exercise phosphocreatine recovery kinetics

Naimon

Walczyk

Babb

et al. 2017

Magn Reson Mater Phy

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Object To develop a low-cost pedal ergometer compatible with ultra high (7 T) field MR systems to reliably quantify metabolic parameters in human lower leg muscle using phosphorus magnetic resonance spectroscopy. Materials and Methods We constructed an MR compatible ergometer using commercially available materials and elastic bands that provide resistance to movement. We recruited 10 healthy subjects (eight men and two women, mean age ± standard deviation: 32.8 ± 6.0 years, BMI: 24.1 ± 3.9 kg/m2). All subjects were scanned on a 7 T whole-body magnet. Each subject was scanned on two visits and performed a 90 s plantar flexion exercise at 40 % maximum voluntary contraction during each scan. During the first visit, each subject performed the exercise twice in order for us to estimate the intra-exam repeatability, and once during the second visit in order to estimate the inter-exam repeatability of the time constant of phosphocreatine recovery kinetics. We assessed the intra- and inter-exam repeatability in terms of the within-subject coefficient of variation (CV). Results We acquired reliable measurements of PCr recovery kinetics with an intra- and inter-exam CV of 7.9% and 5.7%, respectively. Conclusion We constructed a low-cost pedal ergometer compatible with ultra high (7 T) field MR systems, which allowed us to reliably quantify PCr recovery kinetics in lower leg muscle using 31P-MRS.

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Dynamic ³¹P MR spectroscopy of plantar flexion: Influence of ergometer design, magnetic field strength (3 and 7 T), and RF‐coil design

Cited by 30 publications

References 55 publications

Skeletal muscle ATP synthesis and cellular H+ handling measured by localized 31P-MRS during exercise and recovery

Skeletal muscle ATP synthesis and cellular H+ handling measured by localized 31P-MRS during exercise and recovery

In-vivo 31P-MRS of skeletal muscle and liver: A way for non-invasive assessment of their metabolism

A low-cost Mr compatible ergometer to assess post-exercise phosphocreatine recovery kinetics

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Dynamic 31P MR spectroscopy of plantar flexion: Influence of ergometer design, magnetic field strength (3 and 7 T), and RF‐coil design

Cited by 30 publications

References 55 publications

Skeletal muscle ATP synthesis and cellular H+ handling measured by localized 31P-MRS during exercise and recovery

Skeletal muscle ATP synthesis and cellular H+ handling measured by localized 31P-MRS during exercise and recovery

In-vivo 31P-MRS of skeletal muscle and liver: A way for non-invasive assessment of their metabolism

A low-cost Mr compatible ergometer to assess post-exercise phosphocreatine recovery kinetics

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Product

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Dynamic ³¹P MR spectroscopy of plantar flexion: Influence of ergometer design, magnetic field strength (3 and 7 T), and RF‐coil design