Dynamic turnover of arabinogalactan proteins in cultured Arabidopsis cells

Darjania, Levan; Ichise, Nobutoshi; Ichikawa, S.; Okamoto, Takashi; Okuyama, Hidetoshi; Thompson, Guy A.

doi:10.1016/s0981-9428(01)01336-5

Cited by 14 publications

(13 citation statements)

References 44 publications

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“…These observations suggest that extracellular AGPs may be required by B. vulgaris cells during cell division. This result is in agreement with the results reported for plant cell cultures of A. thaliana (Darjania et al 2002) and Rosa sp. (Komalavilas et al 1991).…”

Section: Discussionsupporting

confidence: 93%

“…Other studies showed that the increase of these molecules in the culture medium of Arabidopsis thaliana cell culture was closely correlated with the increase in cell number. AGP secretion may involve dynamic changes of AGP sets regulated partially by external factors (Darjania et al 2002). Zhu et al (1993) reported that salt stress causes reduction of the growth rate of Nicotiana tabacum BY-2 cells and decrease of AGP content in plasma membrane and in its release rate to culture medium.…”

Section: Introductionmentioning

confidence: 99%

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Sucrose induces arabinogalactan protein secretion by Beta vulgaris L. cell suspension cultures

et al. 2010

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The aim of this study was to determine if the increase of the initial sucrose concentration (ISC) improves cell growth and arabinogalactan protein (AGP) secretion of Beta vulgaris L. cultures. ISC tested were 43.8, 87.6 and 131.4 mM. Cell growth and specific growth rate were improved increasing the ISC. Cell cultures grown with ISC 43.8 mM were fed with sucrose, and cellular growth was enhanced twofold, revealing the stimulatory effect of sucrose on cell growth. The AGP secretion was stimulated, increasing the ISC. This event was partially associated with the exponential growth phase of the culture. AGP precipitation with Yariv reagent of cell cultures inhibited cell growth without changes in viability. The assay of sucrose feeding confirmed the relationship between cell growth and AGP secretion. These observations suggest that AGPs may be required for cell division. The increase of AGP secretion by ISC coincided with a higher cellular aggregation, suggesting a possible role of AGP as cellular adhesion molecules. To determine whether AGP secretion is also stimulated by an osmotic effect, mannitol was fed to raise the osmotic potential from 23.78 to 95.97 mOsm kg -1 . Mannitol was not used for cell growth, but AGP secretion was stimulated sixfold in relation to the control. These results are important for understanding the possible factors involved in the AGP secretion of plant cell culture and that may be considered to improve the AGP production.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Sucrose induces arabinogalactan protein secretion by Beta vulgaris L. cell suspension cultures

et al. 2010

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“…2), reaching a maximum of 51.5 mg L -1 after 21 days of culture, suggesting that a link may exist between cell growth and Arabinogalactan-proteins secretion. Similar results were reported for Arabidopsis thaliana (Darjania et al 2002) andB. vulgaris (Capataz-Tafur et al 2010).…”

Section: Extracellular Compounds and Emulsifying Propertiessupporting

confidence: 87%

Establishment and characterization of Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. cell suspension culture: a biotechnology approach for mesquite gum production

et al. 2011

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This study presents a protocol for the establishment of Prosopis laevigata cell suspension culture as a strategy to obtain an in vitro mesquite gum productive cell line. The callus used for this purpose was obtained with hypocotyls from 15-day-old plantlets, placed on Murashige-Skoog medium with two different plant growth regulators (PGRs), 2,4,5-trichlorophenoxy acetic acid (2,4,5-T; 5.0 lM) and kinetin (KIN; 5.0 lM). With this PGRs treatment, after four subcultures (30 days each) an exuded gum-like substance was observed on the callus surface. The growth kinetics of the cell suspension culture showed a specific cell growth rate (l) of 0.14 d -1 and doubling time (t d ) of 6.6 days, respectively. The gum-like substance from callus culture and the broth from cell suspension culture were subjected to chemical analysis and compared with the mesquite gum exuded from wild trees. Both, gum-like substance from callus culture and the broth from cell suspension culture showed the presence of Arabinogalactanproteins, and their polysaccharide fraction presented the same monosaccharides as those isolated from mesquite gum. In addition, the emulsifying properties of gum-like substance from callus culture and the broth from cell suspension culture were compared to those of mesquite gum and all three samples exhibited similar emulsifying capacity and emulsification stability.

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“…Recent work with Arabidopsis mutants suggests functions of certain AGPs in cell expansion (Shi et al, 2003), seed germination, in vitro root regeneration (Van Hengel and Roberts, 2003), and response to abscisic acid (Johnson et al, 2003;Van Hengel and Roberts, 2003). Based on the rapid turnover rate of AGPs (Takeuchi and Komamine, 1980;Gibeaut and Carpita, 1991;Darjania et al, 2002), it has been hypothesized that AGPs may function to prevent aggregation of newly synthesized cell wall polymers in the Golgi and keep these polymers soluble inside secretory vesicles on the way to wall deposition (Gibeaut and Carpita, 1991). (Yariv et al, 1967;Nothnagel, 1997 (award no.…”

mentioning

confidence: 99%

Binding of Arabinogalactan Proteins by Yariv Phenylglycoside Triggers Wound-Like Responses in Arabidopsis Cell Cultures

Nothnagel

2004

Plant Physiology

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Arabinogalactan-proteins (AGPs) are cell wall proteoglycans and are widely distributed in the plant kingdom. Classical AGPs and some nonclassical AGPs are predicted to have a glycosylphosphatidylinositol lipid anchor and have been suggested to be involved in cell-cell signaling. Yariv phenylglycoside is a synthetic probe that specifically binds to plant AGPs and has been used to study AGP functions. We treated Arabidopsis suspension cell cultures with Yariv phenylglycoside and observed decreased cell viability, increased cell wall apposition and cytoplasmic vesiculation, and induction of callose deposition. The induction of cell wall apposition and callose synthesis led us to hypothesize that Yariv binding of plant surface AGPs triggers wound-like responses. To study the effect of Yariv binding to plant surface AGPs and to further understand AGP functions, an Arabidopsis whole genome array was used to monitor the transcriptional modifications after Yariv treatment. By comparing the genes that are induced by Yariv treatment with genes whose expressions have been previously shown to be induced by other conditions, we conclude that the gene expression profile induced by Yariv phenylglycoside treatment is most similar to that of wound induction. It remains uncertain whether the Yariv phenylglycoside cross-linking of cell surface AGPs induces these genes through a specific AGP-based signaling mechanism or through a general mechanical perturbation of the cell surface.Arabinogalactan-proteins (AGPs) are widely distributed in plant species and are located at the plasma membrane and cell wall and in the media of cell cultures. These proteoglycans are typically composed of at least 90% carbohydrate by weight. The AGP core polypeptide is usually rich in Hyp, Ser, Thr, and Ala. Extended motifs comparable to those of extensins are not generally found in AGPs, although short stretches of Hyp alternating with Ala or Ser occur in many AGPs. The sugar moieties are composed of (1/3)-b-D-galactan backbones and (1/6)-b-D-galactan side chains with terminal sugars of Ara or GlcUA (Nothnagel, 1997). In the classical AGPs, the nascent polypeptide chain is synthesized with a C-terminal hydrophobic sequence that is later replaced with a glycosylphosphatidylinositol lipid anchor in the mature protein (Gaspar et al., 2001). The Arabidopsis genome contains approximately 47 genes encoding AGP core polypeptides (Schultz et al., 2002).The abundance of AGP genes and the high degree of posttranslational modifications of AGPs suggest a high genome investment in the synthesis of AGPs, which indicates that these macromolecules have conserved and important roles in plants. Although several possible roles of AGPs have been suggested (MajewskaSawka and Nothnagel, 2000), the detailed biological functions of AGPs currently remain unknown. Many experiments have demonstrated that the expression of AGPs is developmentally regulated in tissueand organ-specific manners (Majewska-Sawka and Nothnagel, 2000). Other experiments showed that AGPs are involved in s...

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Dynamic turnover of arabinogalactan proteins in cultured Arabidopsis cells

Cited by 14 publications

References 44 publications

Sucrose induces arabinogalactan protein secretion by Beta vulgaris L. cell suspension cultures

Sucrose induces arabinogalactan protein secretion by Beta vulgaris L. cell suspension cultures

Establishment and characterization of Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. cell suspension culture: a biotechnology approach for mesquite gum production

Binding of Arabinogalactan Proteins by Yariv Phenylglycoside Triggers Wound-Like Responses in Arabidopsis Cell Cultures

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