Dynamically coated capillaries improve the identification power of capillary zone electrophoresis for basic drugs in toxicological analysis

Boone, C.M; Jonkers, Elles; Franke, J.P.; Zeeuw, Rokus A. de; Ensing, K

doi:10.1016/s0021-9673(01)01100-1

Cited by 36 publications

(17 citation statements)

References 13 publications

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“…Such dynamic double coatings feature sulfonic acid functional groups in the second layer and thus exhibit a strong EOF towards the cathode over the entire pH range. They have been successfully employed for the CZE analysis of low-molecular-mass compounds [15][16][17][18], peptides [19], proteins [20] and carbohydrate-deficient transferrin in human serum [21][22][23], to name but a few. Furthermore, the determination of pKa values of drugs [24] and enantioselective separations [25] have recently been reported.…”

Section: Introductionmentioning

confidence: 99%

ITP in dynamically double‐coated fused‐silica capillaries

Caslavska

Thormann

2006

Electrophoresis

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Bidirectional ITP in fused-silica capillaries double-coated with Polybrene and poly-(vinylsulfonate) is a robust approach for analysis of low-molecular-mass compounds. EOF towards the cathode is strong (mobility >4.0 x 10(-8) m(2)/Vs) within the entire pH range investigated (2.40-8.08), dependent on ionic strength and buffer used and, at constant ionic strength, higher at alkaline pH. Electrokinetic separations and transport in such coated capillaries can be described with a dynamic computer model which permits the combined simulation of electrophoresis and electroosmosis in which the EOF is predicted either with a constant (i.e. pH- and ionic strength-independent) or a pH- and ionic strength-dependent electroosmotic mobility. Detector profiles predicted by computer simulation agree qualitatively well with bidirectional isotachopherograms that are monitored with a setup comprising two axial contactless conductivity detectors and a UV absorbance detector. The varying EOF predicted with a pH- and ionic strength-dependent electroosmotic mobility can be regarded as being realistic.

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Section: Introductionmentioning

confidence: 99%

ITP in dynamically double‐coated fused‐silica capillaries

Caslavska

Thormann

2006

Electrophoresis

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“…Various analytical methods were used for determination of DX with different drugs such as PN (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15), CX (16), and PH (7,14,(17)(18)(19) by liquid chromatography (LC) and by capillary electrophoresis with PH (20,21).…”

Section: Introductionmentioning

confidence: 99%

LC for Analysis of Two Sustained-Release Mixtures Containing Cough Cold Suppressant Drugs

El‐Gindy

Sallam

Salam

2010

Journal of Chromatographic Science

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“…The coating procedure involves an initial coating of a polycation, followed by a polyanion. Various types of polycations and polyanions have been previously utilized, including poly(diallyldimethylammonium chloride) (PDDAC) and dextran sulfate (DS) [4,5], and CElixir reagents (A and B) [3,6,7].…”

Section: Introductionmentioning

confidence: 99%

A fast CE method for the achiral separation of methadone and its major metabolites, 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine and 2‐ethyl‐5‐methyl‐3,3‐diphenyl‐1‐pyrroline

2007

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The utilization of dynamic doubly coated capillaries for a fast separation of methadone and its two major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP) was investigated. The coated capillaries were prepared using a polycation of poly(diallyldimethylammonium chloride) and a polyanion of dextran sulfate. A fast achiral separation was developed using the coated capillaries with a BGE of 100 mM phosphate buffer at pH 2.6. Complete achiral separation of methadone, EDDP and EMDP was achieved, with migration times of approximately 4 min. The method offers considerable advantages with respect to BGE simplicity and analysis time compared to previously published CE methods for methadone and its related analytes.

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Dynamically coated capillaries improve the identification power of capillary zone electrophoresis for basic drugs in toxicological analysis

Cited by 36 publications

References 13 publications

ITP in dynamically double‐coated fused‐silica capillaries

ITP in dynamically double‐coated fused‐silica capillaries

LC for Analysis of Two Sustained-Release Mixtures Containing Cough Cold Suppressant Drugs

A fast CE method for the achiral separation of methadone and its major metabolites, 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine and 2‐ethyl‐5‐methyl‐3,3‐diphenyl‐1‐pyrroline

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