ITP in dynamically double‐coated fused‐silica capillaries

Caslavska, Jitka; Thormann, Wolfgang

doi:10.1002/elps.200600346

Cited by 19 publications

(20 citation statements)

References 32 publications

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“…The same was found to be true for the prediction of ITP in PMMA capillaries [129]. Furthermore, recent work by Caslavska and Thormann revealed that fused-silica capillaries double coated with Polybrene and poly(vinylsulfonate) feature a strong cathodic EOF across the entire pH range such that bidirectional ITP zone patterns at acidic pH reach the detector without the addition of imposed flow [153].…”

Section: Aspects Of Itp and Mbesupporting

confidence: 62%

Dynamic computer simulations of electrophoresis: A versatile research and teaching tool

et al. 2010

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Software is available, which simulates all basic electrophoretic systems, including moving boundary electrophoresis, zone electrophoresis, ITP, IEF and EKC, and their combinations under almost exactly the same conditions used in the laboratory. These dynamic models are based upon equations derived from the transport concepts such as electromigration, diffusion, electroosmosis and imposed hydrodynamic buffer flow that are applied to user-specified initial distributions of analytes and electrolytes. They are able to predict the evolution of electrolyte systems together with associated properties such as pH and conductivity profiles and are as such the most versatile tool to explore the fundamentals of electrokinetic separations and analyses. In addition to revealing the detailed mechanisms of fundamental phenomena that occur in electrophoretic separations, dynamic simulations are useful for educational purposes. This review includes a list of current high-resolution simulators, information on how a simulation is performed, simulation examples for zone electrophoresis, ITP, IEF and EKC and a comprehensive discussion of the applications and achievements.

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Section: Aspects Of Itp and Mbesupporting

confidence: 62%

Dynamic computer simulations of electrophoresis: A versatile research and teaching tool

et al. 2010

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“…This was divided into 20 000 equal segments with a constant voltage of 2500 V (500 V/ cm, the equivalent field strength used by Sueyoshi et À9 m 2 /Vs) was set using the model described in Caslavska et al [21]. For clarity, injection and micellar volumes will only be discussed relative to the 25-mm capillary length.…”

Section: Methodsmentioning

confidence: 99%

Insight into the mechanism of transient trapping in micellar electrokinetic chromatography

2011

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Transient trapping is a new mechanism of on-line sample concentration and separation that has recently been presented. It involves the injection of a short length of micellar solution in front of the sample, making it similar to sweeping in partial-filling MEKC. Here, we examine the mechanism of transient trapping by the use of computer simulations and compare it to sweeping in MEKC for the two analytes, sulforhodamine B and 101. The simulation results confirm the mechanism for concentration and separation originally proposed. The mechanism for concentration is similar to sweeping since the analytes are picked and accumulated by the micelles that penetrate the sample zone. The mechanism for separation is however quite unique since the concentrated analytes are trapped for a few seconds on the sample/micelle boundary before they are released as the concentration of micelle is reduced as it undergoes electromigration dispersion and the analytes separate down a micelle gradient. Simulation results suggested that a significant contribution of band broadening arises from the micelle gradient, with shallower gradients resulting in broader peaks. However, this is offset by an increase in selectivity, such that resolution was enhanced even though the peaks are broader. Transient trapping analysis with similar resolution to those obtained by sweeping MEKC could be achieved in 1/10 of the time and 1/4 of the capillary length, which results in a 2-3 times increase in sensitivity.

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“…For measuring the low EOF and the imposed flow generated via application of pressure during electrophoresis in an LPA coated capillary, a setup with a C 4 D as described elsewhere was employed. Briefly, it comprised a PrinCE sampler (model 560, Prince Technologies, Emmen, The Netherlands), a 50 μm id capillary of 90 cm total length and the detector at 47 % of capillary length.…”

Section: Methodsmentioning

confidence: 99%

“…GENTRANS permits the combined simulation of the temporal behavior of electrophoresis and electroosmosis under constant voltage or current conditions and was previously used to estimate the pH and ionic strength dependent EOF in uncoated fused‐silica and polymethylmethacrylate (PMMA) capillaries . In another effort, the pH independent EOF in dynamically double coated fused‐silica capillaries featuring a charged surface was studied in the same way . Furthermore, this dynamic CE model which uses material or surface property specific input data for estimation of electroosmosis was applied to investigate fundamental aspects of isoelectric focusing in capillaries or microchannels made from bare fused‐silica, fused‐silica coated with a sulfonated polymer, PMMA and poly(dimethylsiloxane) .…”

Section: Introductionmentioning

confidence: 99%

Validation of CE modeling with a contactless conductivity array detector

et al. 2016

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Dynamic computer simulation data are compared for the first time with CE data obtained with a laboratory made system comprising an array of 8 contactless conductivity detectors (C(4) Ds). The experimental setup featured a 50 μm id linear polyacrylamide (LPA) coated fused-silica capillary of 70 cm length and a purpose built sequential injection analysis manifold for fluid handling of continuous or discontinuous buffer configurations and sample injection. The LPA coated capillary exhibits a low EOF and the manifold allows the placement of the first detector at about 2.7 cm from the sample inlet. Agreement of simulated electropherograms with experimental data was obtained for the migration and separation of cationic and anionic analyte and system zones in CZE configurations in which EOF and other column properties are constant. For configurations with discontinuous buffer systems, including ITP, experimental data obtained with the array detector revealed that the EOF is not constant. Comparison of simulation and experimental data of ITP systems provided the insight that the EOF can be estimated with an ionic strength dependent model similar to that previously used to describe EOF in fused-silica capillaries dynamically double coated with Polybrene and poly(vinylsulfonate). For the LPA coated capillaries, the electroosmotic mobility was determined to be 17-fold smaller compared to the case with the charged double coating. Simulation and array detection provide means for quickly investigating electrophoretic transport and separation properties. Without realistic input parameters, modeling alone is not providing data that match CE results.

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ITP in dynamically double‐coated fused‐silica capillaries

Cited by 19 publications

References 32 publications

Dynamic computer simulations of electrophoresis: A versatile research and teaching tool

Dynamic computer simulations of electrophoresis: A versatile research and teaching tool

Insight into the mechanism of transient trapping in micellar electrokinetic chromatography

Validation of CE modeling with a contactless conductivity array detector

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